N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes
Oxidative stress plays critical roles in the pathogenesis of diabetes, hypertension, and atherosclerosis; some authors reported that fat accumulation correlates to systemic oxidative stress in human and mice, but cellular redox environment effect on lipid accumulation is still unclear. In our labora...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Publicado: |
2016
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_22132317_v9_n_p39_Pieralisi http://hdl.handle.net/20.500.12110/paper_22132317_v9_n_p39_Pieralisi |
| Aporte de: |
| id |
paper:paper_22132317_v9_n_p39_Pieralisi |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_22132317_v9_n_p39_Pieralisi2023-06-08T16:35:17Z N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes Martini, Claudia Noemí Vila, María del Carmen Calvo, Juan Carlos Guerra, Liliana Noemí Adipogenesis Antioxidants Kinases Lipids MEF N-acetylcysteine acetylcysteine Janus kinase mitogen activated protein kinase phosphoERK phosphoJNK phosphoprotein triacylglycerol unclassified drug acetylcysteine phosphotransferase adipocyte adipogenesis animal cell animal tissue antioxidant activity Article cell count cell differentiation cell function cell level cell proliferation cell stress cell viability concentration response controlled study disease model drug cytotoxicity drug effect embryo enzyme phosphorylation lipid storage mitotic clonal expansion mouse nonhuman obesity priority journal procedures concerning cells protein expression protein lipid interaction signal transduction 3T3-L1 cell line adipocyte animal cytology drug effects fibroblast lipid metabolism mammalian embryo metabolism phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Adipogenesis Animals Cell Differentiation Embryo, Mammalian Fibroblasts Lipid Metabolism Mice Phosphorylation Phosphotransferases Oxidative stress plays critical roles in the pathogenesis of diabetes, hypertension, and atherosclerosis; some authors reported that fat accumulation correlates to systemic oxidative stress in human and mice, but cellular redox environment effect on lipid accumulation is still unclear. In our laboratory we used mouse embryonic fibroblasts (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC) and accumulate lipids, as obesity model. Here we analyzed the role of the well-known antioxidant and glutathione precursor N-acetylcysteine (NAC) in cellular MAPK modulation and lipid accumulation. We evaluated the effect of NAC on the adipogenic differentiation pathway using different doses: 0.01, 0.1, 1 and 5 mM; no toxic doses in these cells. A dose of 5 mM NAC [DCN-5] provoked a significant decrease in triglyceride accumulation (72±10 [DCN-5] vs 169±15 [DC], p<0.01), as well in Oil Red O stained neutral lipid content (120±2 [DCN-5] vs 139±12 [DC], p<0.01). Molecular mechanisms responsible for adipogenic differentiation involve increase of the expression of phosphoERK1/2 and phosphoJNK, 5 mM NAC treatment inhibited both pERK1/2 and pJNK protein levels. We also evaluated the mitotic clonal expansion (MCE) which takes place during adipogenesis and observed an increase in DC at a rate of 1.5 cells number compared to CC at day 2, whereas the highest doses of NAC significantly inhibited MCE. Our results suggest that NAC inhibits lipid accumulation and the MAPK phosphorylation in mouse embryonic fibroblasts during adipogenic differentiation and further contribute to probe the importance of cellular redox environment in adipogenesis. © 2016 The Authors. Fil:Martini, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vila, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerra, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_22132317_v9_n_p39_Pieralisi http://hdl.handle.net/20.500.12110/paper_22132317_v9_n_p39_Pieralisi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adipogenesis Antioxidants Kinases Lipids MEF N-acetylcysteine acetylcysteine Janus kinase mitogen activated protein kinase phosphoERK phosphoJNK phosphoprotein triacylglycerol unclassified drug acetylcysteine phosphotransferase adipocyte adipogenesis animal cell animal tissue antioxidant activity Article cell count cell differentiation cell function cell level cell proliferation cell stress cell viability concentration response controlled study disease model drug cytotoxicity drug effect embryo enzyme phosphorylation lipid storage mitotic clonal expansion mouse nonhuman obesity priority journal procedures concerning cells protein expression protein lipid interaction signal transduction 3T3-L1 cell line adipocyte animal cytology drug effects fibroblast lipid metabolism mammalian embryo metabolism phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Adipogenesis Animals Cell Differentiation Embryo, Mammalian Fibroblasts Lipid Metabolism Mice Phosphorylation Phosphotransferases |
| spellingShingle |
Adipogenesis Antioxidants Kinases Lipids MEF N-acetylcysteine acetylcysteine Janus kinase mitogen activated protein kinase phosphoERK phosphoJNK phosphoprotein triacylglycerol unclassified drug acetylcysteine phosphotransferase adipocyte adipogenesis animal cell animal tissue antioxidant activity Article cell count cell differentiation cell function cell level cell proliferation cell stress cell viability concentration response controlled study disease model drug cytotoxicity drug effect embryo enzyme phosphorylation lipid storage mitotic clonal expansion mouse nonhuman obesity priority journal procedures concerning cells protein expression protein lipid interaction signal transduction 3T3-L1 cell line adipocyte animal cytology drug effects fibroblast lipid metabolism mammalian embryo metabolism phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Adipogenesis Animals Cell Differentiation Embryo, Mammalian Fibroblasts Lipid Metabolism Mice Phosphorylation Phosphotransferases Martini, Claudia Noemí Vila, María del Carmen Calvo, Juan Carlos Guerra, Liliana Noemí N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes |
| topic_facet |
Adipogenesis Antioxidants Kinases Lipids MEF N-acetylcysteine acetylcysteine Janus kinase mitogen activated protein kinase phosphoERK phosphoJNK phosphoprotein triacylglycerol unclassified drug acetylcysteine phosphotransferase adipocyte adipogenesis animal cell animal tissue antioxidant activity Article cell count cell differentiation cell function cell level cell proliferation cell stress cell viability concentration response controlled study disease model drug cytotoxicity drug effect embryo enzyme phosphorylation lipid storage mitotic clonal expansion mouse nonhuman obesity priority journal procedures concerning cells protein expression protein lipid interaction signal transduction 3T3-L1 cell line adipocyte animal cytology drug effects fibroblast lipid metabolism mammalian embryo metabolism phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Adipogenesis Animals Cell Differentiation Embryo, Mammalian Fibroblasts Lipid Metabolism Mice Phosphorylation Phosphotransferases |
| description |
Oxidative stress plays critical roles in the pathogenesis of diabetes, hypertension, and atherosclerosis; some authors reported that fat accumulation correlates to systemic oxidative stress in human and mice, but cellular redox environment effect on lipid accumulation is still unclear. In our laboratory we used mouse embryonic fibroblasts (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC) and accumulate lipids, as obesity model. Here we analyzed the role of the well-known antioxidant and glutathione precursor N-acetylcysteine (NAC) in cellular MAPK modulation and lipid accumulation. We evaluated the effect of NAC on the adipogenic differentiation pathway using different doses: 0.01, 0.1, 1 and 5 mM; no toxic doses in these cells. A dose of 5 mM NAC [DCN-5] provoked a significant decrease in triglyceride accumulation (72±10 [DCN-5] vs 169±15 [DC], p<0.01), as well in Oil Red O stained neutral lipid content (120±2 [DCN-5] vs 139±12 [DC], p<0.01). Molecular mechanisms responsible for adipogenic differentiation involve increase of the expression of phosphoERK1/2 and phosphoJNK, 5 mM NAC treatment inhibited both pERK1/2 and pJNK protein levels. We also evaluated the mitotic clonal expansion (MCE) which takes place during adipogenesis and observed an increase in DC at a rate of 1.5 cells number compared to CC at day 2, whereas the highest doses of NAC significantly inhibited MCE. Our results suggest that NAC inhibits lipid accumulation and the MAPK phosphorylation in mouse embryonic fibroblasts during adipogenic differentiation and further contribute to probe the importance of cellular redox environment in adipogenesis. © 2016 The Authors. |
| author |
Martini, Claudia Noemí Vila, María del Carmen Calvo, Juan Carlos Guerra, Liliana Noemí |
| author_facet |
Martini, Claudia Noemí Vila, María del Carmen Calvo, Juan Carlos Guerra, Liliana Noemí |
| author_sort |
Martini, Claudia Noemí |
| title |
N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes |
| title_short |
N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes |
| title_full |
N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes |
| title_fullStr |
N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes |
| title_full_unstemmed |
N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes |
| title_sort |
n-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_22132317_v9_n_p39_Pieralisi http://hdl.handle.net/20.500.12110/paper_22132317_v9_n_p39_Pieralisi |
| work_keys_str_mv |
AT martiniclaudianoemi nacetylcysteineinhibitslipidaccumulationinmouseembryonicadipocytes AT vilamariadelcarmen nacetylcysteineinhibitslipidaccumulationinmouseembryonicadipocytes AT calvojuancarlos nacetylcysteineinhibitslipidaccumulationinmouseembryonicadipocytes AT guerraliliananoemi nacetylcysteineinhibitslipidaccumulationinmouseembryonicadipocytes |
| _version_ |
1768544661379481600 |