Novel red fluorophores with superior performance in STED microscopy

In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine ba...

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Detalles Bibliográficos
Publicado:	2012
Materias:	Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines STED Stimulated emission depletion Super-resolution Bio-conjugation Super resolution Fluorescence microscopy Fluorophores Microscopic examination Optical microscopy Phosphorylation Quantum yield Stimulated emission
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21922853_v1_n1_p1_Wurm http://hdl.handle.net/20.500.12110/paper_21922853_v1_n1_p1_Wurm
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_21922853_v1_n1_p1_Wurm
record_format	dspace
spelling	paper:paper_21922853_v1_n1_p1_Wurm2023-06-08T16:35:06Z Novel red fluorophores with superior performance in STED microscopy Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines STED Stimulated emission depletion Super-resolution Bio-conjugation Confocal Nanoscopy Rhodamines STED Stimulated emission depletion Super resolution Fluorescence Fluorescence microscopy Fluorophores Microscopic examination Optical microscopy Phosphorylation Quantum yield Stimulated emission In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21922853_v1_n1_p1_Wurm http://hdl.handle.net/20.500.12110/paper_21922853_v1_n1_p1_Wurm
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines STED Stimulated emission depletion Super-resolution Bio-conjugation Confocal Nanoscopy Rhodamines STED Stimulated emission depletion Super resolution Fluorescence Fluorescence microscopy Fluorophores Microscopic examination Optical microscopy Phosphorylation Quantum yield Stimulated emission
spellingShingle	Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines STED Stimulated emission depletion Super-resolution Bio-conjugation Confocal Nanoscopy Rhodamines STED Stimulated emission depletion Super resolution Fluorescence Fluorescence microscopy Fluorophores Microscopic examination Optical microscopy Phosphorylation Quantum yield Stimulated emission Novel red fluorophores with superior performance in STED microscopy
topic_facet	Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines STED Stimulated emission depletion Super-resolution Bio-conjugation Confocal Nanoscopy Rhodamines STED Stimulated emission depletion Super resolution Fluorescence Fluorescence microscopy Fluorophores Microscopic examination Optical microscopy Phosphorylation Quantum yield Stimulated emission
description	In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al.
title	Novel red fluorophores with superior performance in STED microscopy
title_short	Novel red fluorophores with superior performance in STED microscopy
title_full	Novel red fluorophores with superior performance in STED microscopy
title_fullStr	Novel red fluorophores with superior performance in STED microscopy
title_full_unstemmed	Novel red fluorophores with superior performance in STED microscopy
title_sort	novel red fluorophores with superior performance in sted microscopy
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21922853_v1_n1_p1_Wurm http://hdl.handle.net/20.500.12110/paper_21922853_v1_n1_p1_Wurm
_version_	1768541961530114048

Novel red fluorophores with superior performance in STED microscopy

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