Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae)
The funnel-web spider Macrothele calpeiana is a charismatic Mygalomorph with a great interest in basic, applied and translational research. Nevertheless, current scarcity of genomic and transcriptomic data of this species clearly limits the research in this non-model organism. To overcome this limit...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21678359_v2015_n6_p_FriasLopez http://hdl.handle.net/20.500.12110/paper_21678359_v2015_n6_p_FriasLopez |
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paper:paper_21678359_v2015_n6_p_FriasLopez2023-06-08T16:34:32Z Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae) Chemosensory system De novo transcriptome assembly Molecular markers Mygalomorphae Phylogeny RNA-seq complementary DNA tissue specific transcriptome transcriptome unclassified drug adult Article biodiversity bootstrapping chemosensory related gene chemosensory tissue data processing DNA marker female gene expression gene ontology gene sequence genomics housekeeping gene hybridization Macrothele calpeiana molecular biology molecular genetics nonhuman phylogeny RNA extraction RNA sequence spider The funnel-web spider Macrothele calpeiana is a charismatic Mygalomorph with a great interest in basic, applied and translational research. Nevertheless, current scarcity of genomic and transcriptomic data of this species clearly limits the research in this non-model organism. To overcome this limitation, we launched the first tissue-specific enriched RNA-seq analysis in this species using a subtractive hybridization approach, with two main objectives, to characterize the specific transcriptome of the putative chemosensory appendages (palps and first pair of legs), and to provide a new set of DNA markers for further phylogenetic studies. We have characterized the set of transcripts specifically expressed in putative chemosensory tissues of this species, much of them showing features shared by chemosensory system genes. Among specific candidates, we have identified some members of the iGluR and NPC2 families. Moreover, we have demonstrated the utility of these newly generated data as molecular markers by inferring the phylogenetic position M. calpeina in the phylogenetic tree of Mygalomorphs. Our results provide novel resources for researchers interested in spider molecular biology and systematics, which can help to expand our knowledge on the evolutionary processes underlying fundamental biological questions, as species invasion or biodiversity origin and maintenance. © 2015 Frías-López et al. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21678359_v2015_n6_p_FriasLopez http://hdl.handle.net/20.500.12110/paper_21678359_v2015_n6_p_FriasLopez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chemosensory system De novo transcriptome assembly Molecular markers Mygalomorphae Phylogeny RNA-seq complementary DNA tissue specific transcriptome transcriptome unclassified drug adult Article biodiversity bootstrapping chemosensory related gene chemosensory tissue data processing DNA marker female gene expression gene ontology gene sequence genomics housekeeping gene hybridization Macrothele calpeiana molecular biology molecular genetics nonhuman phylogeny RNA extraction RNA sequence spider |
| spellingShingle |
Chemosensory system De novo transcriptome assembly Molecular markers Mygalomorphae Phylogeny RNA-seq complementary DNA tissue specific transcriptome transcriptome unclassified drug adult Article biodiversity bootstrapping chemosensory related gene chemosensory tissue data processing DNA marker female gene expression gene ontology gene sequence genomics housekeeping gene hybridization Macrothele calpeiana molecular biology molecular genetics nonhuman phylogeny RNA extraction RNA sequence spider Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae) |
| topic_facet |
Chemosensory system De novo transcriptome assembly Molecular markers Mygalomorphae Phylogeny RNA-seq complementary DNA tissue specific transcriptome transcriptome unclassified drug adult Article biodiversity bootstrapping chemosensory related gene chemosensory tissue data processing DNA marker female gene expression gene ontology gene sequence genomics housekeeping gene hybridization Macrothele calpeiana molecular biology molecular genetics nonhuman phylogeny RNA extraction RNA sequence spider |
| description |
The funnel-web spider Macrothele calpeiana is a charismatic Mygalomorph with a great interest in basic, applied and translational research. Nevertheless, current scarcity of genomic and transcriptomic data of this species clearly limits the research in this non-model organism. To overcome this limitation, we launched the first tissue-specific enriched RNA-seq analysis in this species using a subtractive hybridization approach, with two main objectives, to characterize the specific transcriptome of the putative chemosensory appendages (palps and first pair of legs), and to provide a new set of DNA markers for further phylogenetic studies. We have characterized the set of transcripts specifically expressed in putative chemosensory tissues of this species, much of them showing features shared by chemosensory system genes. Among specific candidates, we have identified some members of the iGluR and NPC2 families. Moreover, we have demonstrated the utility of these newly generated data as molecular markers by inferring the phylogenetic position M. calpeina in the phylogenetic tree of Mygalomorphs. Our results provide novel resources for researchers interested in spider molecular biology and systematics, which can help to expand our knowledge on the evolutionary processes underlying fundamental biological questions, as species invasion or biodiversity origin and maintenance. © 2015 Frías-López et al. |
| title |
Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae) |
| title_short |
Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae) |
| title_full |
Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae) |
| title_fullStr |
Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae) |
| title_full_unstemmed |
Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae) |
| title_sort |
comparative analysis of tissue-specific transcriptomes in the funnel-web spider macrothele calpeiana (araneae, hexathelidae) |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21678359_v2015_n6_p_FriasLopez http://hdl.handle.net/20.500.12110/paper_21678359_v2015_n6_p_FriasLopez |
| _version_ |
1768546466066857984 |