A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells

We report the synthesis of a near-infrared (NIR) fluorescent pH probe with a remarkable Stokes shift (∼135 nm) based on a tricarbocyanine (Cy-PIP). The fluorescent molecule was anchored to SiO2 nanoparticles (Cy-PIP@SiO2) and is capable of monitoring pH changes within the physiological range (pH 6-8...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Coluccio Leskow, Federico, Bordoni, Andrea Verónica, Acebedo, Sofìa Lorena, Spagnuolo, Carla Cecilia
Publicado:	2017
Materias:	Infrared devices Nanoparticles Silica Endocytic pathways Fluorescence confocal microscopy Fluorescent molecules Fluorescent pH probes Near-infrared fluorescence pH fluctuations Physiological range SiO2 Nanoparticles Fluorescence
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20507518_v5_n22_p4031_ToumTerrones http://hdl.handle.net/20.500.12110/paper_20507518_v5_n22_p4031_ToumTerrones
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_20507518_v5_n22_p4031_ToumTerrones
record_format	dspace
spelling	paper:paper_20507518_v5_n22_p4031_ToumTerrones2023-06-08T16:33:54Z A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells Coluccio Leskow, Federico Bordoni, Andrea Verónica Acebedo, Sofìa Lorena Spagnuolo, Carla Cecilia Infrared devices Nanoparticles Silica Endocytic pathways Fluorescence confocal microscopy Fluorescent molecules Fluorescent pH probes Near-infrared fluorescence pH fluctuations Physiological range SiO2 Nanoparticles Fluorescence We report the synthesis of a near-infrared (NIR) fluorescent pH probe with a remarkable Stokes shift (∼135 nm) based on a tricarbocyanine (Cy-PIP). The fluorescent molecule was anchored to SiO2 nanoparticles (Cy-PIP@SiO2) and is capable of monitoring pH changes within the physiological range (pH 6-8). The Cy-PIP@SiO2 nanoparticles were successfully internalized by HeLa cells as shown by fluorescence confocal microscopy, while flow cytometry revealed pH fluctuations during the endocytic pathway. © 2017 The Royal Society of Chemistry. Fil:Coluccio Leskow, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bordoni, A.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Acebedo, S.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Spagnuolo, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20507518_v5_n22_p4031_ToumTerrones http://hdl.handle.net/20.500.12110/paper_20507518_v5_n22_p4031_ToumTerrones
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Infrared devices Nanoparticles Silica Endocytic pathways Fluorescence confocal microscopy Fluorescent molecules Fluorescent pH probes Near-infrared fluorescence pH fluctuations Physiological range SiO2 Nanoparticles Fluorescence
spellingShingle	Infrared devices Nanoparticles Silica Endocytic pathways Fluorescence confocal microscopy Fluorescent molecules Fluorescent pH probes Near-infrared fluorescence pH fluctuations Physiological range SiO2 Nanoparticles Fluorescence Coluccio Leskow, Federico Bordoni, Andrea Verónica Acebedo, Sofìa Lorena Spagnuolo, Carla Cecilia A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
topic_facet	Infrared devices Nanoparticles Silica Endocytic pathways Fluorescence confocal microscopy Fluorescent molecules Fluorescent pH probes Near-infrared fluorescence pH fluctuations Physiological range SiO2 Nanoparticles Fluorescence
description	We report the synthesis of a near-infrared (NIR) fluorescent pH probe with a remarkable Stokes shift (∼135 nm) based on a tricarbocyanine (Cy-PIP). The fluorescent molecule was anchored to SiO2 nanoparticles (Cy-PIP@SiO2) and is capable of monitoring pH changes within the physiological range (pH 6-8). The Cy-PIP@SiO2 nanoparticles were successfully internalized by HeLa cells as shown by fluorescence confocal microscopy, while flow cytometry revealed pH fluctuations during the endocytic pathway. © 2017 The Royal Society of Chemistry.
author	Coluccio Leskow, Federico Bordoni, Andrea Verónica Acebedo, Sofìa Lorena Spagnuolo, Carla Cecilia
author_facet	Coluccio Leskow, Federico Bordoni, Andrea Verónica Acebedo, Sofìa Lorena Spagnuolo, Carla Cecilia
author_sort	Coluccio Leskow, Federico
title	A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
title_short	A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
title_full	A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
title_fullStr	A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
title_full_unstemmed	A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells
title_sort	silica supported tricarbocyanine based ph nanosensor with a large stokes shift and a near infrared fluorescence response: performance in vitro and in live cells
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20507518_v5_n22_p4031_ToumTerrones http://hdl.handle.net/20.500.12110/paper_20507518_v5_n22_p4031_ToumTerrones
work_keys_str_mv	AT coluccioleskowfederico asilicasupportedtricarbocyaninebasedphnanosensorwithalargestokesshiftandanearinfraredfluorescenceresponseperformanceinvitroandinlivecells AT bordoniandreaveronica asilicasupportedtricarbocyaninebasedphnanosensorwithalargestokesshiftandanearinfraredfluorescenceresponseperformanceinvitroandinlivecells AT acebedosofialorena asilicasupportedtricarbocyaninebasedphnanosensorwithalargestokesshiftandanearinfraredfluorescenceresponseperformanceinvitroandinlivecells AT spagnuolocarlacecilia asilicasupportedtricarbocyaninebasedphnanosensorwithalargestokesshiftandanearinfraredfluorescenceresponseperformanceinvitroandinlivecells AT coluccioleskowfederico silicasupportedtricarbocyaninebasedphnanosensorwithalargestokesshiftandanearinfraredfluorescenceresponseperformanceinvitroandinlivecells AT bordoniandreaveronica silicasupportedtricarbocyaninebasedphnanosensorwithalargestokesshiftandanearinfraredfluorescenceresponseperformanceinvitroandinlivecells AT acebedosofialorena silicasupportedtricarbocyaninebasedphnanosensorwithalargestokesshiftandanearinfraredfluorescenceresponseperformanceinvitroandinlivecells AT spagnuolocarlacecilia silicasupportedtricarbocyaninebasedphnanosensorwithalargestokesshiftandanearinfraredfluorescenceresponseperformanceinvitroandinlivecells
_version_	1768543822061502464

A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: Performance in vitro and in live cells

Ejemplares similares