Single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells
Most accepted single particle tracking methods are able to obtain high-resolution trajectories for relatively short periods of time. In this work we apply a straightforward combination of single-particle tracking microscopy and metallic nanoparticles internalization on mouse chromaffin cells to unve...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20506120_v6_n1_p_Gabriel http://hdl.handle.net/20.500.12110/paper_20506120_v6_n1_p_Gabriel |
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paper:paper_20506120_v6_n1_p_Gabriel |
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paper:paper_20506120_v6_n1_p_Gabriel2023-06-08T16:33:52Z Single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells Fluorescence Intracellular dynamics Metallic nanoparticles Microscopy New labels for cell imaging Single particle tracking Two-photon chlorpromazine clathrin gold metal nanoparticle potassium animal cell culture chromaffin cell drug effect endocytosis female male metabolism mouse single molecule imaging Animals Cells, Cultured Chlorpromazine Chromaffin Cells Clathrin Endocytosis Female Gold Male Metal Nanoparticles Mice Potassium Single Molecule Imaging Most accepted single particle tracking methods are able to obtain high-resolution trajectories for relatively short periods of time. In this work we apply a straightforward combination of single-particle tracking microscopy and metallic nanoparticles internalization on mouse chromaffin cells to unveil the intracellular trafficking mechanism of metallic-nanoparticle-loaded vesicles (MNP-V) complexes after clathrin dependent endocytosis. We found that directed transport is the major route of MNP-Vs intracellular trafficking after stimulation (92.6% of the trajectories measured). We then studied the MNP-V speed at each point along the trajectory, and found that the application of a second depolarization stimulus during the tracking provokes an increase in the percentage of low-speed trajectory points in parallel with a decrease in the number of high-speed trajectory points. This result suggests that stimulation may facilitate the compartmentalization of internalized MNPs in a more restricted location such as was already demonstrated in neuronal and neuroendocrine cells (Bronfman et al 2003 J. Neurosci. 23 3209-20). Although further experiments will be required to address the mechanisms underlying this transport dynamics, our studies provide quantitative evidence of the heterogeneous behavior of vesicles mobility after endocytosis in chromaffin cells highlighting the potential of MNPs as alternative labels in optical microscopy to provide new insights into the vesicles dynamics in a wide variety of cellular environments. © 2017 IOP Publishing Ltd. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20506120_v6_n1_p_Gabriel http://hdl.handle.net/20.500.12110/paper_20506120_v6_n1_p_Gabriel |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fluorescence Intracellular dynamics Metallic nanoparticles Microscopy New labels for cell imaging Single particle tracking Two-photon chlorpromazine clathrin gold metal nanoparticle potassium animal cell culture chromaffin cell drug effect endocytosis female male metabolism mouse single molecule imaging Animals Cells, Cultured Chlorpromazine Chromaffin Cells Clathrin Endocytosis Female Gold Male Metal Nanoparticles Mice Potassium Single Molecule Imaging |
| spellingShingle |
Fluorescence Intracellular dynamics Metallic nanoparticles Microscopy New labels for cell imaging Single particle tracking Two-photon chlorpromazine clathrin gold metal nanoparticle potassium animal cell culture chromaffin cell drug effect endocytosis female male metabolism mouse single molecule imaging Animals Cells, Cultured Chlorpromazine Chromaffin Cells Clathrin Endocytosis Female Gold Male Metal Nanoparticles Mice Potassium Single Molecule Imaging Single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells |
| topic_facet |
Fluorescence Intracellular dynamics Metallic nanoparticles Microscopy New labels for cell imaging Single particle tracking Two-photon chlorpromazine clathrin gold metal nanoparticle potassium animal cell culture chromaffin cell drug effect endocytosis female male metabolism mouse single molecule imaging Animals Cells, Cultured Chlorpromazine Chromaffin Cells Clathrin Endocytosis Female Gold Male Metal Nanoparticles Mice Potassium Single Molecule Imaging |
| description |
Most accepted single particle tracking methods are able to obtain high-resolution trajectories for relatively short periods of time. In this work we apply a straightforward combination of single-particle tracking microscopy and metallic nanoparticles internalization on mouse chromaffin cells to unveil the intracellular trafficking mechanism of metallic-nanoparticle-loaded vesicles (MNP-V) complexes after clathrin dependent endocytosis. We found that directed transport is the major route of MNP-Vs intracellular trafficking after stimulation (92.6% of the trajectories measured). We then studied the MNP-V speed at each point along the trajectory, and found that the application of a second depolarization stimulus during the tracking provokes an increase in the percentage of low-speed trajectory points in parallel with a decrease in the number of high-speed trajectory points. This result suggests that stimulation may facilitate the compartmentalization of internalized MNPs in a more restricted location such as was already demonstrated in neuronal and neuroendocrine cells (Bronfman et al 2003 J. Neurosci. 23 3209-20). Although further experiments will be required to address the mechanisms underlying this transport dynamics, our studies provide quantitative evidence of the heterogeneous behavior of vesicles mobility after endocytosis in chromaffin cells highlighting the potential of MNPs as alternative labels in optical microscopy to provide new insights into the vesicles dynamics in a wide variety of cellular environments. © 2017 IOP Publishing Ltd. |
| title |
Single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells |
| title_short |
Single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells |
| title_full |
Single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells |
| title_fullStr |
Single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells |
| title_full_unstemmed |
Single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells |
| title_sort |
single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20506120_v6_n1_p_Gabriel http://hdl.handle.net/20.500.12110/paper_20506120_v6_n1_p_Gabriel |
| _version_ |
1768541632370573312 |