Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry

In the present work we show that a recent methodology developed by us to acquire emission spectra and fluorescence quantum yields of highly absorbing samples in transmission configuration, constitutes a very simple and robust alternative to determine self-quenching constants, KSQ. We measured the ab...

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Detalles Bibliográficos
Publicado: 2017
Materias:
Fluorescence
Quinine
Re-absorption
Self-quenching
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20506120_v5_n3_p_Krimer
http://hdl.handle.net/20.500.12110/paper_20506120_v5_n3_p_Krimer
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_20506120_v5_n3_p_Krimer
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spelling paper:paper_20506120_v5_n3_p_Krimer2023-06-08T16:33:51Z Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry Fluorescence Quinine Re-absorption Self-quenching In the present work we show that a recent methodology developed by us to acquire emission spectra and fluorescence quantum yields of highly absorbing samples in transmission configuration, constitutes a very simple and robust alternative to determine self-quenching constants, KSQ. We measured the absorption and the steady-state emission spectra of quinine bisulphate, QBS, solutions ranging between 1.5 × 10-5 and 1.5 × 10-1 M. From these data, we calculated the expected emission spectra, affected by re-absorption, for all QBS concentrations. For higher concentrations, the re-absorption in the excitation/detection direction reaches values up to 6% of the total emitted intensity. The KSQ of the dye was reevaluated from the concentration dependence of the quotients between the calculated and the experimental integrated emission spectra. The obtained value, KSQ = 18.4 ± 0.1 M-1, shows no significant differences with those obtained from steady-state and average lifetimes by other authors, pointing out the diffusional nature of the self-quenching phenomenon. The present work helps clarify some ambiguous aspects concerning the photophysics of QBS, stressing that re-absorption phenomena must be considered in QBS concentrated solutions for accuracy measurements. © 2017 IOP Publishing Ltd. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20506120_v5_n3_p_Krimer http://hdl.handle.net/20.500.12110/paper_20506120_v5_n3_p_Krimer
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Fluorescence
Quinine
Re-absorption
Self-quenching
spellingShingle Fluorescence
Quinine
Re-absorption
Self-quenching
Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
topic_facet Fluorescence
Quinine
Re-absorption
Self-quenching
description In the present work we show that a recent methodology developed by us to acquire emission spectra and fluorescence quantum yields of highly absorbing samples in transmission configuration, constitutes a very simple and robust alternative to determine self-quenching constants, KSQ. We measured the absorption and the steady-state emission spectra of quinine bisulphate, QBS, solutions ranging between 1.5 × 10-5 and 1.5 × 10-1 M. From these data, we calculated the expected emission spectra, affected by re-absorption, for all QBS concentrations. For higher concentrations, the re-absorption in the excitation/detection direction reaches values up to 6% of the total emitted intensity. The KSQ of the dye was reevaluated from the concentration dependence of the quotients between the calculated and the experimental integrated emission spectra. The obtained value, KSQ = 18.4 ± 0.1 M-1, shows no significant differences with those obtained from steady-state and average lifetimes by other authors, pointing out the diffusional nature of the self-quenching phenomenon. The present work helps clarify some ambiguous aspects concerning the photophysics of QBS, stressing that re-absorption phenomena must be considered in QBS concentrated solutions for accuracy measurements. © 2017 IOP Publishing Ltd.
title Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
title_short Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
title_full Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
title_fullStr Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
title_full_unstemmed Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
title_sort re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
publishDate 2017
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20506120_v5_n3_p_Krimer
http://hdl.handle.net/20.500.12110/paper_20506120_v5_n3_p_Krimer
_version_ 1768545995930468352

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