Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster
The range of thermal tolerance is one of the main factors influencing the geographic distribution of species. Climate change projections predict increases in average and extreme temperatures over the coming decades; hence, the ability of living beings to resist these changes will depend on physiolog...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20457758_v8_n21_p10374_Rolandi http://hdl.handle.net/20.500.12110/paper_20457758_v8_n21_p10374_Rolandi |
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paper:paper_20457758_v8_n21_p10374_Rolandi2023-06-08T16:33:42Z Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster climatic adaptation CTmax DGRP global warming scenario GWAS SNPs The range of thermal tolerance is one of the main factors influencing the geographic distribution of species. Climate change projections predict increases in average and extreme temperatures over the coming decades; hence, the ability of living beings to resist these changes will depend on physiological and adaptive responses. On an evolutionary scale, changes will occur as the result of selective pressures on individual heritable differences. In this work, we studied the genetic basis of tolerance to high temperatures in the fly Drosophila melanogaster and whether this species presents sufficient genetic variability to allow expansion of its upper thermo-tolerance limit. To do so, we used adult flies derived from a natural population belonging to the Drosophila Genetic Reference Panel, for which genomic sequencing data are available. We characterized the phenotypic variation of the upper thermal limit in 34 lines by measuring knockdown temperature (i.e., critical thermal maximum [CTmax]) by exposing flies to a ramp of increasing temperature (0.25°C/min). Fourteen percent of the variation in CTmax is explained by the genetic variation across lines, without a significant sexual dimorphism. Through a genomewide association study, 12 single nucleotide polymorphisms associated with the CTmax were identified. In most of these SNPs, the less frequent allele increased the upper thermal limit suggesting that this population harbors raw genetic variation capable of expanding its heat tolerance. This potential upper thermal tolerance increase has implications under the global warming scenario. Past climatic records show a very low incidence of days above CTmax (10 days over 25 years); however, future climate scenarios predict 243 days with extreme high temperature above CTmax from 2045 to 2070. Thus, in the context of the future climate warming, rising temperatures might drive the evolution of heat tolerance in this population by increasing the frequency of the alleles associated with higher CTmax. © 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20457758_v8_n21_p10374_Rolandi http://hdl.handle.net/20.500.12110/paper_20457758_v8_n21_p10374_Rolandi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
climatic adaptation CTmax DGRP global warming scenario GWAS SNPs |
| spellingShingle |
climatic adaptation CTmax DGRP global warming scenario GWAS SNPs Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| topic_facet |
climatic adaptation CTmax DGRP global warming scenario GWAS SNPs |
| description |
The range of thermal tolerance is one of the main factors influencing the geographic distribution of species. Climate change projections predict increases in average and extreme temperatures over the coming decades; hence, the ability of living beings to resist these changes will depend on physiological and adaptive responses. On an evolutionary scale, changes will occur as the result of selective pressures on individual heritable differences. In this work, we studied the genetic basis of tolerance to high temperatures in the fly Drosophila melanogaster and whether this species presents sufficient genetic variability to allow expansion of its upper thermo-tolerance limit. To do so, we used adult flies derived from a natural population belonging to the Drosophila Genetic Reference Panel, for which genomic sequencing data are available. We characterized the phenotypic variation of the upper thermal limit in 34 lines by measuring knockdown temperature (i.e., critical thermal maximum [CTmax]) by exposing flies to a ramp of increasing temperature (0.25°C/min). Fourteen percent of the variation in CTmax is explained by the genetic variation across lines, without a significant sexual dimorphism. Through a genomewide association study, 12 single nucleotide polymorphisms associated with the CTmax were identified. In most of these SNPs, the less frequent allele increased the upper thermal limit suggesting that this population harbors raw genetic variation capable of expanding its heat tolerance. This potential upper thermal tolerance increase has implications under the global warming scenario. Past climatic records show a very low incidence of days above CTmax (10 days over 25 years); however, future climate scenarios predict 243 days with extreme high temperature above CTmax from 2045 to 2070. Thus, in the context of the future climate warming, rising temperatures might drive the evolution of heat tolerance in this population by increasing the frequency of the alleles associated with higher CTmax. © 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. |
| title |
Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| title_short |
Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| title_full |
Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| title_fullStr |
Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| title_full_unstemmed |
Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| title_sort |
genetic variation for tolerance to high temperatures in a population of drosophila melanogaster |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20457758_v8_n21_p10374_Rolandi http://hdl.handle.net/20.500.12110/paper_20457758_v8_n21_p10374_Rolandi |
| _version_ |
1768546136050630656 |