Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells

During decidualization, endometrial stromal cells undergo reticular stress (RS) and unfolded protein response (UPR), allowing the endoplasmic reticulum-expansion and immunomodulators production. Physiological RS generates the activation of sensing proteins, inflammasome activation and mature-IL-1β s...

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Detalles Bibliográficos
Publicado: 2018
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v8_n1_p_Grasso
http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_Grasso
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_20452322_v8_n1_p_Grasso
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spelling paper:paper_20452322_v8_n1_p_Grasso2023-06-08T16:33:35Z Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells During decidualization, endometrial stromal cells undergo reticular stress (RS) and unfolded protein response (UPR), allowing the endoplasmic reticulum-expansion and immunomodulators production. Physiological RS generates the activation of sensing proteins, inflammasome activation and mature-IL-1β secretion, associated with pro-implantatory effects. We focus on the impact of RS and UPR on decidualized cells and whether they induce a physiological sterile inflammatory response through IL-1β production. Human endometrial stromal cell line (HESC) after decidualization treatment with MPA + dibutyryl-cAMP (Dec) increased the expression of RS-sensors (ATF6, PERK and IRE1α) and UPR markers (sXBP1 and CHOP) in comparison with Non-dec cells. Then we found increased NLRP3 expression in Dec cells compared with Non-dec cells. In fact STF-083010 (an IRE1α inhibitor) prevented this increase. Downstream, increased levels of active caspase-1 on Dec cells were detected by FAM-Flica Caspase-1 associated with an increase in IL-1β production. Moreover, the treatment with STF-083010 decreased the invasion index observed in Dec cells, evaluated by an in vitro model of implantation. In endometrial biopsies from recurrent spontaneous abortion patients an increased expression of IRE1α was found in comparison with fertile women; while recurrent implantation failure samples showed a lower expression of sXBP1, TXNIP and NLRP3 than fertile women, suggesting that RS/UPR tenors might condition endometrial receptivity. © 2018, The Author(s). 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v8_n1_p_Grasso http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_Grasso
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description During decidualization, endometrial stromal cells undergo reticular stress (RS) and unfolded protein response (UPR), allowing the endoplasmic reticulum-expansion and immunomodulators production. Physiological RS generates the activation of sensing proteins, inflammasome activation and mature-IL-1β secretion, associated with pro-implantatory effects. We focus on the impact of RS and UPR on decidualized cells and whether they induce a physiological sterile inflammatory response through IL-1β production. Human endometrial stromal cell line (HESC) after decidualization treatment with MPA + dibutyryl-cAMP (Dec) increased the expression of RS-sensors (ATF6, PERK and IRE1α) and UPR markers (sXBP1 and CHOP) in comparison with Non-dec cells. Then we found increased NLRP3 expression in Dec cells compared with Non-dec cells. In fact STF-083010 (an IRE1α inhibitor) prevented this increase. Downstream, increased levels of active caspase-1 on Dec cells were detected by FAM-Flica Caspase-1 associated with an increase in IL-1β production. Moreover, the treatment with STF-083010 decreased the invasion index observed in Dec cells, evaluated by an in vitro model of implantation. In endometrial biopsies from recurrent spontaneous abortion patients an increased expression of IRE1α was found in comparison with fertile women; while recurrent implantation failure samples showed a lower expression of sXBP1, TXNIP and NLRP3 than fertile women, suggesting that RS/UPR tenors might condition endometrial receptivity. © 2018, The Author(s).
title Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells
spellingShingle Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells
title_short Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells
title_full Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells
title_fullStr Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells
title_full_unstemmed Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells
title_sort impact of the reticular stress and unfolded protein response on the inflammatory response in endometrial stromal cells
publishDate 2018
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v8_n1_p_Grasso
http://hdl.handle.net/20.500.12110/paper_20452322_v8_n1_p_Grasso
_version_ 1768542908557819904

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