Vasoactive Intestinal Peptide modulates trophoblast-derived cell line function and interaction with phagocytic cells through autocrine pathways
Trophoblast cells migrate and invade the decidual stroma in a tightly regulated process to maintain immune homeostasis at the maternal-placental interface during the first weeks of pregnancy. Locally synthesized factors modulate trophoblast cell function and their interaction with maternal leukocyte...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v6_n_p_Vota http://hdl.handle.net/20.500.12110/paper_20452322_v6_n_p_Vota |
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paper:paper_20452322_v6_n_p_Vota2023-06-08T16:33:28Z Vasoactive Intestinal Peptide modulates trophoblast-derived cell line function and interaction with phagocytic cells through autocrine pathways hypophysis adenylate cyclase activating polypeptide receptor protein kinase vasoactive intestinal polypeptide autocrine effect cell line cell motion cytology female first trimester pregnancy gene knockdown genetics human metabolism phagocyte pregnancy signal transduction trophoblast Autocrine Communication Cell Line Cell Movement Female Gene Knockdown Techniques Humans Phagocytes Pregnancy Pregnancy Trimester, First Protein Kinases Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide Signal Transduction Trophoblasts Vasoactive Intestinal Peptide Trophoblast cells migrate and invade the decidual stroma in a tightly regulated process to maintain immune homeostasis at the maternal-placental interface during the first weeks of pregnancy. Locally synthesized factors modulate trophoblast cell function and their interaction with maternal leukocytes to promote the silent clearance of apoptotic cells. The vasoactive intestinal peptide (VIP) is a pleiotropic polypeptide with trophic and anti-inflammatory effects in murine pregnancy models. We explored the effect of VIP on two human first trimester trophoblast cell lines, particularly on their migration, invasiveness and interaction with phagocytic cells, and the signalling and regulatory pathways involved. We found that VIP enhanced trophoblast cell migration and invasion through the activation of high affinity VPAC receptors and PKA-CRE signalling pathways. VIP knocked-down trophoblast cells showed reduced migration in basal and leukemic inhibitor factor (LIF)-elicited conditions. In parallel, VIP-silenced trophoblast cells failed to induce the phagocytosis of apoptotic bodies and the expression of immunosuppressant markers by human monocytes. Our results suggest that VIP-mediated autocrine pathways regulate trophoblast cell function and contribute to immune homeostasis maintenance at placentation and may provide new clues for therapeutic intervention in pregnancies complicated by defective deep placentation. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v6_n_p_Vota http://hdl.handle.net/20.500.12110/paper_20452322_v6_n_p_Vota |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
hypophysis adenylate cyclase activating polypeptide receptor protein kinase vasoactive intestinal polypeptide autocrine effect cell line cell motion cytology female first trimester pregnancy gene knockdown genetics human metabolism phagocyte pregnancy signal transduction trophoblast Autocrine Communication Cell Line Cell Movement Female Gene Knockdown Techniques Humans Phagocytes Pregnancy Pregnancy Trimester, First Protein Kinases Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide Signal Transduction Trophoblasts Vasoactive Intestinal Peptide |
| spellingShingle |
hypophysis adenylate cyclase activating polypeptide receptor protein kinase vasoactive intestinal polypeptide autocrine effect cell line cell motion cytology female first trimester pregnancy gene knockdown genetics human metabolism phagocyte pregnancy signal transduction trophoblast Autocrine Communication Cell Line Cell Movement Female Gene Knockdown Techniques Humans Phagocytes Pregnancy Pregnancy Trimester, First Protein Kinases Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide Signal Transduction Trophoblasts Vasoactive Intestinal Peptide Vasoactive Intestinal Peptide modulates trophoblast-derived cell line function and interaction with phagocytic cells through autocrine pathways |
| topic_facet |
hypophysis adenylate cyclase activating polypeptide receptor protein kinase vasoactive intestinal polypeptide autocrine effect cell line cell motion cytology female first trimester pregnancy gene knockdown genetics human metabolism phagocyte pregnancy signal transduction trophoblast Autocrine Communication Cell Line Cell Movement Female Gene Knockdown Techniques Humans Phagocytes Pregnancy Pregnancy Trimester, First Protein Kinases Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide Signal Transduction Trophoblasts Vasoactive Intestinal Peptide |
| description |
Trophoblast cells migrate and invade the decidual stroma in a tightly regulated process to maintain immune homeostasis at the maternal-placental interface during the first weeks of pregnancy. Locally synthesized factors modulate trophoblast cell function and their interaction with maternal leukocytes to promote the silent clearance of apoptotic cells. The vasoactive intestinal peptide (VIP) is a pleiotropic polypeptide with trophic and anti-inflammatory effects in murine pregnancy models. We explored the effect of VIP on two human first trimester trophoblast cell lines, particularly on their migration, invasiveness and interaction with phagocytic cells, and the signalling and regulatory pathways involved. We found that VIP enhanced trophoblast cell migration and invasion through the activation of high affinity VPAC receptors and PKA-CRE signalling pathways. VIP knocked-down trophoblast cells showed reduced migration in basal and leukemic inhibitor factor (LIF)-elicited conditions. In parallel, VIP-silenced trophoblast cells failed to induce the phagocytosis of apoptotic bodies and the expression of immunosuppressant markers by human monocytes. Our results suggest that VIP-mediated autocrine pathways regulate trophoblast cell function and contribute to immune homeostasis maintenance at placentation and may provide new clues for therapeutic intervention in pregnancies complicated by defective deep placentation. |
| title |
Vasoactive Intestinal Peptide modulates trophoblast-derived cell line function and interaction with phagocytic cells through autocrine pathways |
| title_short |
Vasoactive Intestinal Peptide modulates trophoblast-derived cell line function and interaction with phagocytic cells through autocrine pathways |
| title_full |
Vasoactive Intestinal Peptide modulates trophoblast-derived cell line function and interaction with phagocytic cells through autocrine pathways |
| title_fullStr |
Vasoactive Intestinal Peptide modulates trophoblast-derived cell line function and interaction with phagocytic cells through autocrine pathways |
| title_full_unstemmed |
Vasoactive Intestinal Peptide modulates trophoblast-derived cell line function and interaction with phagocytic cells through autocrine pathways |
| title_sort |
vasoactive intestinal peptide modulates trophoblast-derived cell line function and interaction with phagocytic cells through autocrine pathways |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v6_n_p_Vota http://hdl.handle.net/20.500.12110/paper_20452322_v6_n_p_Vota |
| _version_ |
1768543774165696512 |