Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors
Photoswitchable molecules and nanoparticles constitute superior biosensors for a wide range of industrial, research and biomedical applications. Rendered reversible by spontaneous or deterministic means, such probes facilitate many of the techniques in fluorescence microscopy that surpass the optica...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20411723_v6_n_p_Diaz http://hdl.handle.net/20.500.12110/paper_20411723_v6_n_p_Diaz |
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paper:paper_20411723_v6_n_p_Diaz2023-06-08T16:33:07Z Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors nanorod polymer quantum dot crystal structure polymer probe quantum mechanics resonance semiconductor industry Article fluorescence resonance energy transfer light absorption photoactivation quantum yield regulatory mechanism surface property ultraviolet radiation Photoswitchable molecules and nanoparticles constitute superior biosensors for a wide range of industrial, research and biomedical applications. Rendered reversible by spontaneous or deterministic means, such probes facilitate many of the techniques in fluorescence microscopy that surpass the optical resolution dictated by diffraction. Here we have devised a family of photoswitchable quantum dots (psQDs) in which the semiconductor core functions as a fluorescence donor in Förster resonance energy transfer (FRET), and multiple photochromic diheteroarylethene groups function as acceptors upon activation by ultraviolet light. The QDs were coated with a polymer bearing photochromic groups attached via linkers of different length. Despite the resulting nominal differences in donor-acceptor separation and anticipated FRET efficiencies, the maximum quenching of all psQD preparations was 38±2%. This result was attributable to the large ultraviolet absorption cross-section of the QDs, leading to preferential cycloreversion of photochromic groups situated closer to the nanoparticle surface and/or with a more favourable orientation. © 2015 Macmillan Publishers Limited. All rights reserved. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20411723_v6_n_p_Diaz http://hdl.handle.net/20.500.12110/paper_20411723_v6_n_p_Diaz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
nanorod polymer quantum dot crystal structure polymer probe quantum mechanics resonance semiconductor industry Article fluorescence resonance energy transfer light absorption photoactivation quantum yield regulatory mechanism surface property ultraviolet radiation |
| spellingShingle |
nanorod polymer quantum dot crystal structure polymer probe quantum mechanics resonance semiconductor industry Article fluorescence resonance energy transfer light absorption photoactivation quantum yield regulatory mechanism surface property ultraviolet radiation Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors |
| topic_facet |
nanorod polymer quantum dot crystal structure polymer probe quantum mechanics resonance semiconductor industry Article fluorescence resonance energy transfer light absorption photoactivation quantum yield regulatory mechanism surface property ultraviolet radiation |
| description |
Photoswitchable molecules and nanoparticles constitute superior biosensors for a wide range of industrial, research and biomedical applications. Rendered reversible by spontaneous or deterministic means, such probes facilitate many of the techniques in fluorescence microscopy that surpass the optical resolution dictated by diffraction. Here we have devised a family of photoswitchable quantum dots (psQDs) in which the semiconductor core functions as a fluorescence donor in Förster resonance energy transfer (FRET), and multiple photochromic diheteroarylethene groups function as acceptors upon activation by ultraviolet light. The QDs were coated with a polymer bearing photochromic groups attached via linkers of different length. Despite the resulting nominal differences in donor-acceptor separation and anticipated FRET efficiencies, the maximum quenching of all psQD preparations was 38±2%. This result was attributable to the large ultraviolet absorption cross-section of the QDs, leading to preferential cycloreversion of photochromic groups situated closer to the nanoparticle surface and/or with a more favourable orientation. © 2015 Macmillan Publishers Limited. All rights reserved. |
| title |
Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors |
| title_short |
Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors |
| title_full |
Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors |
| title_fullStr |
Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors |
| title_full_unstemmed |
Photoswitchable semiconductor nanocrystals with self-regulating photochromic Förster resonance energy transfer acceptors |
| title_sort |
photoswitchable semiconductor nanocrystals with self-regulating photochromic förster resonance energy transfer acceptors |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20411723_v6_n_p_Diaz http://hdl.handle.net/20.500.12110/paper_20411723_v6_n_p_Diaz |
| _version_ |
1768542243885416448 |