ESIPT and FRET probes for monitoring nanoparticle polymer coating stability

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Coating strategies of inorganic nanoparticles (NPs) can provide properties unavailable to the NP core alone, such as targeting, specific sensing, and increased biocompatibility. Non-covalent amphiphilic NP capping polymers function via hydrophobic interactions with surface ligands and are extensivel...

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Autor principal: Fauerbach, Jonathan Arturo
Publicado: 2017
Materias:
Biocompatibility
Coatings
Energy transfer
Excited states
Hydrophobicity
Ionic strength
Nanoparticles
Polymers
Stability
System stability
Adjustable parameters
Amphiphilic polymers
Coating instability
Complex environments
Excited-state intramolecular proton transfer
Hydrophobic interactions
Inorganic nanoparticle
Resonance energy transfer
Plastic coatings
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20403364_v9_n25_p8647_Azcarate
http://hdl.handle.net/20.500.12110/paper_20403364_v9_n25_p8647_Azcarate
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_20403364_v9_n25_p8647_Azcarate
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spelling paper:paper_20403364_v9_n25_p8647_Azcarate2023-06-08T16:33:03Z ESIPT and FRET probes for monitoring nanoparticle polymer coating stability Fauerbach, Jonathan Arturo Biocompatibility Coatings Energy transfer Excited states Hydrophobicity Ionic strength Nanoparticles Polymers Stability System stability Adjustable parameters Amphiphilic polymers Coating instability Complex environments Excited-state intramolecular proton transfer Hydrophobic interactions Inorganic nanoparticle Resonance energy transfer Plastic coatings Coating strategies of inorganic nanoparticles (NPs) can provide properties unavailable to the NP core alone, such as targeting, specific sensing, and increased biocompatibility. Non-covalent amphiphilic NP capping polymers function via hydrophobic interactions with surface ligands and are extensively used to transfer NPs to aqueous media. For applications of coated NPs as actuators (sensors, markers, or for drug delivery) in a complex environment, such as biological systems, it is important to achieve a deep understanding of the factors affecting coating stability and behavior. We have designed a system that tests the coating stability of amphiphilic polymers through a simple fluorescent readout using either polarity sensing ESIPT (excited state intramolecular proton transfer) dyes or NP FRET (Förster resonance energy transfer). The stability of the coating was determined in response to changes in polarity, pH and ionic strength in the medium. Using the ESIPT system we observed linear changes in signal up to ∼20-25% v/v of co-solvent addition, constituting a break point. Based on such data, we propose a model for coating instability and the important adjustable parameters, such as the electrical charge distribution. FRET data provided confirmatory evidence for the model. The ESIPT dyes and FRET based methods represent new, simple tools for testing NP coating stability in complex environments. © 2017 The Royal Society of Chemistry. Fil:Fauerbach, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20403364_v9_n25_p8647_Azcarate http://hdl.handle.net/20.500.12110/paper_20403364_v9_n25_p8647_Azcarate
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Biocompatibility
Coatings
Energy transfer
Excited states
Hydrophobicity
Ionic strength
Nanoparticles
Polymers
Stability
System stability
Adjustable parameters
Amphiphilic polymers
Coating instability
Complex environments
Excited-state intramolecular proton transfer
Hydrophobic interactions
Inorganic nanoparticle
Resonance energy transfer
Plastic coatings
spellingShingle Biocompatibility
Coatings
Energy transfer
Excited states
Hydrophobicity
Ionic strength
Nanoparticles
Polymers
Stability
System stability
Adjustable parameters
Amphiphilic polymers
Coating instability
Complex environments
Excited-state intramolecular proton transfer
Hydrophobic interactions
Inorganic nanoparticle
Resonance energy transfer
Plastic coatings
Fauerbach, Jonathan Arturo
ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
topic_facet Biocompatibility
Coatings
Energy transfer
Excited states
Hydrophobicity
Ionic strength
Nanoparticles
Polymers
Stability
System stability
Adjustable parameters
Amphiphilic polymers
Coating instability
Complex environments
Excited-state intramolecular proton transfer
Hydrophobic interactions
Inorganic nanoparticle
Resonance energy transfer
Plastic coatings
description Coating strategies of inorganic nanoparticles (NPs) can provide properties unavailable to the NP core alone, such as targeting, specific sensing, and increased biocompatibility. Non-covalent amphiphilic NP capping polymers function via hydrophobic interactions with surface ligands and are extensively used to transfer NPs to aqueous media. For applications of coated NPs as actuators (sensors, markers, or for drug delivery) in a complex environment, such as biological systems, it is important to achieve a deep understanding of the factors affecting coating stability and behavior. We have designed a system that tests the coating stability of amphiphilic polymers through a simple fluorescent readout using either polarity sensing ESIPT (excited state intramolecular proton transfer) dyes or NP FRET (Förster resonance energy transfer). The stability of the coating was determined in response to changes in polarity, pH and ionic strength in the medium. Using the ESIPT system we observed linear changes in signal up to ∼20-25% v/v of co-solvent addition, constituting a break point. Based on such data, we propose a model for coating instability and the important adjustable parameters, such as the electrical charge distribution. FRET data provided confirmatory evidence for the model. The ESIPT dyes and FRET based methods represent new, simple tools for testing NP coating stability in complex environments. © 2017 The Royal Society of Chemistry.
author Fauerbach, Jonathan Arturo
author_facet Fauerbach, Jonathan Arturo
author_sort Fauerbach, Jonathan Arturo
title ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_short ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_full ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_fullStr ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_full_unstemmed ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_sort esipt and fret probes for monitoring nanoparticle polymer coating stability
publishDate 2017
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20403364_v9_n25_p8647_Azcarate
http://hdl.handle.net/20.500.12110/paper_20403364_v9_n25_p8647_Azcarate
work_keys_str_mv AT fauerbachjonathanarturo esiptandfretprobesformonitoringnanoparticlepolymercoatingstability
_version_ 1768546181721358336

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