High chromosomal variation in wild horn fly haematobia irritans (Linnaeus) (Diptera, Muscidae) populations

The horn fly, Haematobia irritans is an obligate haematophagous cosmopolitan insect pest. The first reports of attacks on livestock by H. irritans in Argentina and Uruguay occurred in 1991, and since 1993 it is considered an economically important pest. Knowledge on the genetic characteristics of th...

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Autores principales: Rabossi, Alejandro, Quesada Allué, Luis Alberto, Basso, Alicia
Publicado: 2015
Materias:
B-chromosome
Chromosomal rearrangements
Evolution
Genetic variability
H-banding
Karyotypes
Population structure
Diptera
Haematobia irritans
Hexapoda
Muscidae
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19930771_v9_n1_p31_Forneris
http://hdl.handle.net/20.500.12110/paper_19930771_v9_n1_p31_Forneris
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_19930771_v9_n1_p31_Forneris
record_format dspace
spelling paper:paper_19930771_v9_n1_p31_Forneris2023-06-08T16:32:52Z High chromosomal variation in wild horn fly haematobia irritans (Linnaeus) (Diptera, Muscidae) populations Rabossi, Alejandro Quesada Allué, Luis Alberto Basso, Alicia B-chromosome Chromosomal rearrangements Evolution Genetic variability H-banding Karyotypes Population structure Diptera Haematobia irritans Hexapoda Muscidae The horn fly, Haematobia irritans is an obligate haematophagous cosmopolitan insect pest. The first reports of attacks on livestock by H. irritans in Argentina and Uruguay occurred in 1991, and since 1993 it is considered an economically important pest. Knowledge on the genetic characteristics of the horn fly increases our understanding of the phenotypes resistant to insecticides that repeatedly develop in these insects. The karyotype of H. irritans, as previously described using flies from an inbred colony, shows a chromosome complement of 2n=10 without heterochromosomes (sex chromosomes). In this study, we analyze for the first time the chromosome structure and variation of four wild populations of H. irritans recently established in the Southern Cone of South America, collected in Argentina and Uruguay. In these wild type populations, we confirmed and characterized the previously published "standard" karyotype of 2n=10 without sex chromosomes; however, surprisingly a supernumerary element, called B-chromosome, was found in about half of mitotic preparations. The existence of statistically significant karyotypic diversity was demonstrated through the application of orcein staining, C-banding and H-banding. This study represents the first discovery and characterization of horn fly karyotypes with 2n=11 (2n=10+B). All spermatocytes analyzed showed 5 chromosome bivalents, and therefore, 2n=10 without an extra chromosome. Study of mitotic divisions showed that some chromosomal rearrangements affecting karyotype structure are maintained as polymorphisms, and multiple correspondence analyses demonstrated that genetic variation was not associated with geographic distribution. Because it was never observed during male meiosis, we hypothesize that B-chromosome is preferentially transmitted by females and that it might be related to sex determination. © Natalia S. Forneris et al. Fil:Rabossi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Quesada-Allué, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Basso, A.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19930771_v9_n1_p31_Forneris http://hdl.handle.net/20.500.12110/paper_19930771_v9_n1_p31_Forneris
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic B-chromosome
Chromosomal rearrangements
Evolution
Genetic variability
H-banding
Karyotypes
Population structure
Diptera
Haematobia irritans
Hexapoda
Muscidae
spellingShingle B-chromosome
Chromosomal rearrangements
Evolution
Genetic variability
H-banding
Karyotypes
Population structure
Diptera
Haematobia irritans
Hexapoda
Muscidae
Rabossi, Alejandro
Quesada Allué, Luis Alberto
Basso, Alicia
High chromosomal variation in wild horn fly haematobia irritans (Linnaeus) (Diptera, Muscidae) populations
topic_facet B-chromosome
Chromosomal rearrangements
Evolution
Genetic variability
H-banding
Karyotypes
Population structure
Diptera
Haematobia irritans
Hexapoda
Muscidae
description The horn fly, Haematobia irritans is an obligate haematophagous cosmopolitan insect pest. The first reports of attacks on livestock by H. irritans in Argentina and Uruguay occurred in 1991, and since 1993 it is considered an economically important pest. Knowledge on the genetic characteristics of the horn fly increases our understanding of the phenotypes resistant to insecticides that repeatedly develop in these insects. The karyotype of H. irritans, as previously described using flies from an inbred colony, shows a chromosome complement of 2n=10 without heterochromosomes (sex chromosomes). In this study, we analyze for the first time the chromosome structure and variation of four wild populations of H. irritans recently established in the Southern Cone of South America, collected in Argentina and Uruguay. In these wild type populations, we confirmed and characterized the previously published "standard" karyotype of 2n=10 without sex chromosomes; however, surprisingly a supernumerary element, called B-chromosome, was found in about half of mitotic preparations. The existence of statistically significant karyotypic diversity was demonstrated through the application of orcein staining, C-banding and H-banding. This study represents the first discovery and characterization of horn fly karyotypes with 2n=11 (2n=10+B). All spermatocytes analyzed showed 5 chromosome bivalents, and therefore, 2n=10 without an extra chromosome. Study of mitotic divisions showed that some chromosomal rearrangements affecting karyotype structure are maintained as polymorphisms, and multiple correspondence analyses demonstrated that genetic variation was not associated with geographic distribution. Because it was never observed during male meiosis, we hypothesize that B-chromosome is preferentially transmitted by females and that it might be related to sex determination. © Natalia S. Forneris et al.
author Rabossi, Alejandro
Quesada Allué, Luis Alberto
Basso, Alicia
author_facet Rabossi, Alejandro
Quesada Allué, Luis Alberto
Basso, Alicia
author_sort Rabossi, Alejandro
title High chromosomal variation in wild horn fly haematobia irritans (Linnaeus) (Diptera, Muscidae) populations
title_short High chromosomal variation in wild horn fly haematobia irritans (Linnaeus) (Diptera, Muscidae) populations
title_full High chromosomal variation in wild horn fly haematobia irritans (Linnaeus) (Diptera, Muscidae) populations
title_fullStr High chromosomal variation in wild horn fly haematobia irritans (Linnaeus) (Diptera, Muscidae) populations
title_full_unstemmed High chromosomal variation in wild horn fly haematobia irritans (Linnaeus) (Diptera, Muscidae) populations
title_sort high chromosomal variation in wild horn fly haematobia irritans (linnaeus) (diptera, muscidae) populations
publishDate 2015
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19930771_v9_n1_p31_Forneris
http://hdl.handle.net/20.500.12110/paper_19930771_v9_n1_p31_Forneris
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AT quesadaallueluisalberto highchromosomalvariationinwildhornflyhaematobiairritanslinnaeusdipteramuscidaepopulations
AT bassoalicia highchromosomalvariationinwildhornflyhaematobiairritanslinnaeusdipteramuscidaepopulations
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