GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models
GTSE1 over-expression has been reported as a potential marker for metastasis in various types of malignancies, including breast cancer. Despite this, the transcriptional regulation of this protein and the causes of its misregulation in tumors remain largely unknown. The aims of this work were to elu...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v8_n40_p67422_Stelitano http://hdl.handle.net/20.500.12110/paper_19492553_v8_n40_p67422_Stelitano |
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paper:paper_19492553_v8_n40_p67422_Stelitano2023-06-08T16:32:40Z GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models Cell protrusions E2F1 GTSE1 TEAD4 YAP/TAZ GTSE1 protein messenger RNA regulator protein retinoblastoma protein transcription factor transcription factor E2F1 transcription factor HMGA1 transcription factor TAZ transcription factor TEAD4 transcription factor YAP unclassified drug Article cell invasion cell migration cell protrusion cell structure controlled study G2 and S phase expressed 1 gene gene gene expression regulation human major clinical study MDA-MB-157 cell line MDA-MB-231 cell line metastasis mevalonate pathway promoter region signal transduction transcription regulation triple negative breast cancer GTSE1 over-expression has been reported as a potential marker for metastasis in various types of malignancies, including breast cancer. Despite this, the transcriptional regulation of this protein and the causes of its misregulation in tumors remain largely unknown. The aims of this work were to elucidate how GTSE1 is regulated at the transcriptional level and to clarify the mechanism underlying GTSE1-dependent cell functions in triple-negative breast cancer (TNBC). Here, we identified GTSE1 as a novel target gene of the TEAD4 transcription factor, highlighting a role for the YAP and TAZ coactivators in the transcriptional regulation of GTSE1. Moreover, we found that TEAD4 controls the formation of cell protrusions required for cell migration through GTSE1, unveiling a relevant effector role for this protein in the TEAD-dependent cellular functions and confirming TEAD4 role in promoting invasion and metastasis in breast cancer. Finally, we highlighted a role for the pRb-E2F1 pathway in the control of GTSE1 transcription and observed that treatment with drugs targeting the pRb-E2F1 or YAP/ TAZ-TEAD pathways dramatically downregulated the expression levels of GTSE1 and of other genes involved in the formation of metastasis, suggesting their potential use in the treatment of TNBC. © Stelitano et al. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v8_n40_p67422_Stelitano http://hdl.handle.net/20.500.12110/paper_19492553_v8_n40_p67422_Stelitano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell protrusions E2F1 GTSE1 TEAD4 YAP/TAZ GTSE1 protein messenger RNA regulator protein retinoblastoma protein transcription factor transcription factor E2F1 transcription factor HMGA1 transcription factor TAZ transcription factor TEAD4 transcription factor YAP unclassified drug Article cell invasion cell migration cell protrusion cell structure controlled study G2 and S phase expressed 1 gene gene gene expression regulation human major clinical study MDA-MB-157 cell line MDA-MB-231 cell line metastasis mevalonate pathway promoter region signal transduction transcription regulation triple negative breast cancer |
| spellingShingle |
Cell protrusions E2F1 GTSE1 TEAD4 YAP/TAZ GTSE1 protein messenger RNA regulator protein retinoblastoma protein transcription factor transcription factor E2F1 transcription factor HMGA1 transcription factor TAZ transcription factor TEAD4 transcription factor YAP unclassified drug Article cell invasion cell migration cell protrusion cell structure controlled study G2 and S phase expressed 1 gene gene gene expression regulation human major clinical study MDA-MB-157 cell line MDA-MB-231 cell line metastasis mevalonate pathway promoter region signal transduction transcription regulation triple negative breast cancer GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models |
| topic_facet |
Cell protrusions E2F1 GTSE1 TEAD4 YAP/TAZ GTSE1 protein messenger RNA regulator protein retinoblastoma protein transcription factor transcription factor E2F1 transcription factor HMGA1 transcription factor TAZ transcription factor TEAD4 transcription factor YAP unclassified drug Article cell invasion cell migration cell protrusion cell structure controlled study G2 and S phase expressed 1 gene gene gene expression regulation human major clinical study MDA-MB-157 cell line MDA-MB-231 cell line metastasis mevalonate pathway promoter region signal transduction transcription regulation triple negative breast cancer |
| description |
GTSE1 over-expression has been reported as a potential marker for metastasis in various types of malignancies, including breast cancer. Despite this, the transcriptional regulation of this protein and the causes of its misregulation in tumors remain largely unknown. The aims of this work were to elucidate how GTSE1 is regulated at the transcriptional level and to clarify the mechanism underlying GTSE1-dependent cell functions in triple-negative breast cancer (TNBC). Here, we identified GTSE1 as a novel target gene of the TEAD4 transcription factor, highlighting a role for the YAP and TAZ coactivators in the transcriptional regulation of GTSE1. Moreover, we found that TEAD4 controls the formation of cell protrusions required for cell migration through GTSE1, unveiling a relevant effector role for this protein in the TEAD-dependent cellular functions and confirming TEAD4 role in promoting invasion and metastasis in breast cancer. Finally, we highlighted a role for the pRb-E2F1 pathway in the control of GTSE1 transcription and observed that treatment with drugs targeting the pRb-E2F1 or YAP/ TAZ-TEAD pathways dramatically downregulated the expression levels of GTSE1 and of other genes involved in the formation of metastasis, suggesting their potential use in the treatment of TNBC. © Stelitano et al. |
| title |
GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models |
| title_short |
GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models |
| title_full |
GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models |
| title_fullStr |
GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models |
| title_full_unstemmed |
GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models |
| title_sort |
gtse1: a novel tead4-e2f1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v8_n40_p67422_Stelitano http://hdl.handle.net/20.500.12110/paper_19492553_v8_n40_p67422_Stelitano |
| _version_ |
1768545393648336896 |