Stable and high expression of Galectin-8 tightly controls metastatic progression of prostate cancer
Two decades ago, Galectin-8 was described as a prostate carcinoma biomarker since it is only expressed in the neoplastic prostate, but not in the healthy tissue. To date, no biological function has been attributed to Galectin-8 that could explain this differential expression. In this study we silenc...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v8_n27_p44654_Gentilini http://hdl.handle.net/20.500.12110/paper_19492553_v8_n27_p44654_Gentilini |
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paper:paper_19492553_v8_n27_p44654_Gentilini2023-06-08T16:32:40Z Stable and high expression of Galectin-8 tightly controls metastatic progression of prostate cancer Kotler, Mónica Lidia Anoikis resistance Galectins Metastasis Prostate cancer Tumour microenvironment galectin 8 messenger RNA uvomorulin actin filament animal experiment animal model animal tissue anoikis Article carcinogenesis cell adhesion cell aggregation cell migration cell proliferation cell survival circulating tumor cell controlled study endothelium cell filopodium human human cell inguinal lymph node lymph node metastasis male metastasis mouse nonhuman prostate cancer prostate cancer cell line protein expression Two decades ago, Galectin-8 was described as a prostate carcinoma biomarker since it is only expressed in the neoplastic prostate, but not in the healthy tissue. To date, no biological function has been attributed to Galectin-8 that could explain this differential expression. In this study we silenced Galectin-8 in two human prostate cancer cell lines, PC3 and IGR-CaP1, and designed a pre-clinical experimental model that allows monitoring the pathology from its early steps to the long-term metastatic stages. We show for the first time that the natural and conserved expression of Gal-8 in tumour cells is responsible for the metastatic evolution of prostate cancer. In fact, Gal-8 controls the rearrangement of the cytoskeleton and E-Cadherin expression, with a major impact on anoikis and homotypic aggregation of tumour cells, both being essential processes for the survival of circulating tumour cells during metastasis. While localized prostate cancer can be cured, metastatic and advanced disease remains a significant therapeutic challenge, urging for the identification of prognostic markers of the metastatic process. Collectively, our results highlight Galectin-8 as a potential target for anti-metastatic therapy against prostate cancer. Fil:Kotler, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v8_n27_p44654_Gentilini http://hdl.handle.net/20.500.12110/paper_19492553_v8_n27_p44654_Gentilini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Anoikis resistance Galectins Metastasis Prostate cancer Tumour microenvironment galectin 8 messenger RNA uvomorulin actin filament animal experiment animal model animal tissue anoikis Article carcinogenesis cell adhesion cell aggregation cell migration cell proliferation cell survival circulating tumor cell controlled study endothelium cell filopodium human human cell inguinal lymph node lymph node metastasis male metastasis mouse nonhuman prostate cancer prostate cancer cell line protein expression |
| spellingShingle |
Anoikis resistance Galectins Metastasis Prostate cancer Tumour microenvironment galectin 8 messenger RNA uvomorulin actin filament animal experiment animal model animal tissue anoikis Article carcinogenesis cell adhesion cell aggregation cell migration cell proliferation cell survival circulating tumor cell controlled study endothelium cell filopodium human human cell inguinal lymph node lymph node metastasis male metastasis mouse nonhuman prostate cancer prostate cancer cell line protein expression Kotler, Mónica Lidia Stable and high expression of Galectin-8 tightly controls metastatic progression of prostate cancer |
| topic_facet |
Anoikis resistance Galectins Metastasis Prostate cancer Tumour microenvironment galectin 8 messenger RNA uvomorulin actin filament animal experiment animal model animal tissue anoikis Article carcinogenesis cell adhesion cell aggregation cell migration cell proliferation cell survival circulating tumor cell controlled study endothelium cell filopodium human human cell inguinal lymph node lymph node metastasis male metastasis mouse nonhuman prostate cancer prostate cancer cell line protein expression |
| description |
Two decades ago, Galectin-8 was described as a prostate carcinoma biomarker since it is only expressed in the neoplastic prostate, but not in the healthy tissue. To date, no biological function has been attributed to Galectin-8 that could explain this differential expression. In this study we silenced Galectin-8 in two human prostate cancer cell lines, PC3 and IGR-CaP1, and designed a pre-clinical experimental model that allows monitoring the pathology from its early steps to the long-term metastatic stages. We show for the first time that the natural and conserved expression of Gal-8 in tumour cells is responsible for the metastatic evolution of prostate cancer. In fact, Gal-8 controls the rearrangement of the cytoskeleton and E-Cadherin expression, with a major impact on anoikis and homotypic aggregation of tumour cells, both being essential processes for the survival of circulating tumour cells during metastasis. While localized prostate cancer can be cured, metastatic and advanced disease remains a significant therapeutic challenge, urging for the identification of prognostic markers of the metastatic process. Collectively, our results highlight Galectin-8 as a potential target for anti-metastatic therapy against prostate cancer. |
| author |
Kotler, Mónica Lidia |
| author_facet |
Kotler, Mónica Lidia |
| author_sort |
Kotler, Mónica Lidia |
| title |
Stable and high expression of Galectin-8 tightly controls metastatic progression of prostate cancer |
| title_short |
Stable and high expression of Galectin-8 tightly controls metastatic progression of prostate cancer |
| title_full |
Stable and high expression of Galectin-8 tightly controls metastatic progression of prostate cancer |
| title_fullStr |
Stable and high expression of Galectin-8 tightly controls metastatic progression of prostate cancer |
| title_full_unstemmed |
Stable and high expression of Galectin-8 tightly controls metastatic progression of prostate cancer |
| title_sort |
stable and high expression of galectin-8 tightly controls metastatic progression of prostate cancer |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v8_n27_p44654_Gentilini http://hdl.handle.net/20.500.12110/paper_19492553_v8_n27_p44654_Gentilini |
| work_keys_str_mv |
AT kotlermonicalidia stableandhighexpressionofgalectin8tightlycontrolsmetastaticprogressionofprostatecancer |
| _version_ |
1768542103116185600 |