Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway
Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19450877_v6_n272_p_Baltanas http://hdl.handle.net/20.500.12110/paper_19450877_v6_n272_p_Baltanas |
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paper:paper_19450877_v6_n272_p_Baltanas2023-06-08T16:32:30Z Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway Baltanás, Rodrigo Bush, Alan Couto, Alicia Susana Durrieu, Lucia Colman Lerner, Alejandro Ariel glycerol mitogen activated protein kinase mitogen activated protein kinase kinase 1 pheromone protein protein Slt2 unclassified drug adaptation article cell structure cell wall cellular stress response controlled study homeostasis hyperosmotic stress morphogenesis negative feedback nonhuman osmoadaptation osmolarity priority journal steady state turnover time yeast cell Cell Wall MAP Kinase Signaling System Membrane Proteins Mitogen-Activated Protein Kinases Osmosis Pheromones Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the "shmooing" morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment. Fil:Baltanás, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bush, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Couto, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Durrieu, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Colman-Lerner, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19450877_v6_n272_p_Baltanas http://hdl.handle.net/20.500.12110/paper_19450877_v6_n272_p_Baltanas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
glycerol mitogen activated protein kinase mitogen activated protein kinase kinase 1 pheromone protein protein Slt2 unclassified drug adaptation article cell structure cell wall cellular stress response controlled study homeostasis hyperosmotic stress morphogenesis negative feedback nonhuman osmoadaptation osmolarity priority journal steady state turnover time yeast cell Cell Wall MAP Kinase Signaling System Membrane Proteins Mitogen-Activated Protein Kinases Osmosis Pheromones Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins |
| spellingShingle |
glycerol mitogen activated protein kinase mitogen activated protein kinase kinase 1 pheromone protein protein Slt2 unclassified drug adaptation article cell structure cell wall cellular stress response controlled study homeostasis hyperosmotic stress morphogenesis negative feedback nonhuman osmoadaptation osmolarity priority journal steady state turnover time yeast cell Cell Wall MAP Kinase Signaling System Membrane Proteins Mitogen-Activated Protein Kinases Osmosis Pheromones Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Baltanás, Rodrigo Bush, Alan Couto, Alicia Susana Durrieu, Lucia Colman Lerner, Alejandro Ariel Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
| topic_facet |
glycerol mitogen activated protein kinase mitogen activated protein kinase kinase 1 pheromone protein protein Slt2 unclassified drug adaptation article cell structure cell wall cellular stress response controlled study homeostasis hyperosmotic stress morphogenesis negative feedback nonhuman osmoadaptation osmolarity priority journal steady state turnover time yeast cell Cell Wall MAP Kinase Signaling System Membrane Proteins Mitogen-Activated Protein Kinases Osmosis Pheromones Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins |
| description |
Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the "shmooing" morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment. |
| author |
Baltanás, Rodrigo Bush, Alan Couto, Alicia Susana Durrieu, Lucia Colman Lerner, Alejandro Ariel |
| author_facet |
Baltanás, Rodrigo Bush, Alan Couto, Alicia Susana Durrieu, Lucia Colman Lerner, Alejandro Ariel |
| author_sort |
Baltanás, Rodrigo |
| title |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
| title_short |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
| title_full |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
| title_fullStr |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
| title_full_unstemmed |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
| title_sort |
pheromone-induced morphogenesis improves osmoadaptation capacity by activating the hog mapk pathway |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19450877_v6_n272_p_Baltanas http://hdl.handle.net/20.500.12110/paper_19450877_v6_n272_p_Baltanas |
| work_keys_str_mv |
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| _version_ |
1768543581143826432 |