Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution
Herein, we report on the implementation of photofunctional surfaces for the investigation of cellular responses by means of quantitative fluorescence microscopy. The developed substrates are able to produce reactive oxygen species under the fluorescence microscope upon irradiation with visible light...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19448244_v7_n10_p5944_Stegemann http://hdl.handle.net/20.500.12110/paper_19448244_v7_n10_p5944_Stegemann |
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paper:paper_19448244_v7_n10_p5944_Stegemann2023-06-08T16:32:26Z Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution Grecco, Hernán Edgardo apoptosis caspase activity cell death necrosis photoactive surfaces phototherapy quantitative functional microscopy reactive oxygen species Cell death Cells Cytology Diseases Fluorescence Fluorescence microscopy Mobile security Morphology Oxygen Photosensitizers Caspases necrosis Phototherapy Quantitative evaluation Quantitative fluorescence microscopy Reactive oxygen species Spatio-temporal resolution Time resolved fluorescence anisotropy Molecular oxygen nanoparticle reactive oxygen metabolite chemistry fluorescence microscopy HeLa cell line human light metabolism molecular imaging photochemistry procedures radiation response spatiotemporal analysis HeLa Cells Humans Light Microscopy, Fluorescence Molecular Imaging Nanoparticles Photochemistry Reactive Oxygen Species Spatio-Temporal Analysis Herein, we report on the implementation of photofunctional surfaces for the investigation of cellular responses by means of quantitative fluorescence microscopy. The developed substrates are able to produce reactive oxygen species under the fluorescence microscope upon irradiation with visible light, and the behavior of cells grown on these surfaces can be consequently investigated in situ and in real time. Moreover, a suitable methodology is presented to simultaneously monitor phototriggered morphological changes and the associated molecular pathways with spatiotemporal resolution employing time-resolved fluorescence anisotropy at the single cell level. The results showed that morphological changes can be complemented with a quantitative evaluation of the associated molecular signaling cascades for the unambiguous assignment of reactive oxygen species-related photoinduced apoptosis. Indeed, similar phenotypes are associated with different cellular processes. Our methodology facilitates the in vitro design and evaluation of photosensitizers for the treatment of cancer and infectious diseases with the aid of functional fluorescence microscopy. (Chemical Presented). © 2015 American Chemical Society. Fil:Grecco, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19448244_v7_n10_p5944_Stegemann http://hdl.handle.net/20.500.12110/paper_19448244_v7_n10_p5944_Stegemann |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
apoptosis caspase activity cell death necrosis photoactive surfaces phototherapy quantitative functional microscopy reactive oxygen species Cell death Cells Cytology Diseases Fluorescence Fluorescence microscopy Mobile security Morphology Oxygen Photosensitizers Caspases necrosis Phototherapy Quantitative evaluation Quantitative fluorescence microscopy Reactive oxygen species Spatio-temporal resolution Time resolved fluorescence anisotropy Molecular oxygen nanoparticle reactive oxygen metabolite chemistry fluorescence microscopy HeLa cell line human light metabolism molecular imaging photochemistry procedures radiation response spatiotemporal analysis HeLa Cells Humans Light Microscopy, Fluorescence Molecular Imaging Nanoparticles Photochemistry Reactive Oxygen Species Spatio-Temporal Analysis |
| spellingShingle |
apoptosis caspase activity cell death necrosis photoactive surfaces phototherapy quantitative functional microscopy reactive oxygen species Cell death Cells Cytology Diseases Fluorescence Fluorescence microscopy Mobile security Morphology Oxygen Photosensitizers Caspases necrosis Phototherapy Quantitative evaluation Quantitative fluorescence microscopy Reactive oxygen species Spatio-temporal resolution Time resolved fluorescence anisotropy Molecular oxygen nanoparticle reactive oxygen metabolite chemistry fluorescence microscopy HeLa cell line human light metabolism molecular imaging photochemistry procedures radiation response spatiotemporal analysis HeLa Cells Humans Light Microscopy, Fluorescence Molecular Imaging Nanoparticles Photochemistry Reactive Oxygen Species Spatio-Temporal Analysis Grecco, Hernán Edgardo Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution |
| topic_facet |
apoptosis caspase activity cell death necrosis photoactive surfaces phototherapy quantitative functional microscopy reactive oxygen species Cell death Cells Cytology Diseases Fluorescence Fluorescence microscopy Mobile security Morphology Oxygen Photosensitizers Caspases necrosis Phototherapy Quantitative evaluation Quantitative fluorescence microscopy Reactive oxygen species Spatio-temporal resolution Time resolved fluorescence anisotropy Molecular oxygen nanoparticle reactive oxygen metabolite chemistry fluorescence microscopy HeLa cell line human light metabolism molecular imaging photochemistry procedures radiation response spatiotemporal analysis HeLa Cells Humans Light Microscopy, Fluorescence Molecular Imaging Nanoparticles Photochemistry Reactive Oxygen Species Spatio-Temporal Analysis |
| description |
Herein, we report on the implementation of photofunctional surfaces for the investigation of cellular responses by means of quantitative fluorescence microscopy. The developed substrates are able to produce reactive oxygen species under the fluorescence microscope upon irradiation with visible light, and the behavior of cells grown on these surfaces can be consequently investigated in situ and in real time. Moreover, a suitable methodology is presented to simultaneously monitor phototriggered morphological changes and the associated molecular pathways with spatiotemporal resolution employing time-resolved fluorescence anisotropy at the single cell level. The results showed that morphological changes can be complemented with a quantitative evaluation of the associated molecular signaling cascades for the unambiguous assignment of reactive oxygen species-related photoinduced apoptosis. Indeed, similar phenotypes are associated with different cellular processes. Our methodology facilitates the in vitro design and evaluation of photosensitizers for the treatment of cancer and infectious diseases with the aid of functional fluorescence microscopy. (Chemical Presented). © 2015 American Chemical Society. |
| author |
Grecco, Hernán Edgardo |
| author_facet |
Grecco, Hernán Edgardo |
| author_sort |
Grecco, Hernán Edgardo |
| title |
Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution |
| title_short |
Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution |
| title_full |
Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution |
| title_fullStr |
Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution |
| title_full_unstemmed |
Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution |
| title_sort |
photofunctional surfaces for quantitative fluorescence microscopy: monitoring the effects of photogenerated reactive oxygen species at single cell level with spatiotemporal resolution |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19448244_v7_n10_p5944_Stegemann http://hdl.handle.net/20.500.12110/paper_19448244_v7_n10_p5944_Stegemann |
| work_keys_str_mv |
AT greccohernanedgardo photofunctionalsurfacesforquantitativefluorescencemicroscopymonitoringtheeffectsofphotogeneratedreactiveoxygenspeciesatsinglecelllevelwithspatiotemporalresolution |
| _version_ |
1768545760470630400 |