Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy
The importance and widespread incidence of Fusarium poae as a natural contaminant of wheat in different climatic areas warrants investigation into the genetic diversity and toxin profile of a northern Italy population. Eighty-one strains of F. poae isolated from durum wheat kernels, identified by sp...
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paper:paper_19440049_v27_n5_p729_Somma2023-06-08T16:32:23Z Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy Alvarez, Cora Lilia Fungi Fusarium Liquid chromatography/mass spectrometry (LC/MS) Mycology Mycotoxins Plants Polymerase chain reaction (PCR) Trichothecenes beauvericin elongation factor 1alpha fusarenon X trichothecene article controlled study food contamination fungal colonization fungal contamination fungal gene fungal genetics fungal plant disease fungal strain fungus isolation Fusarium Fusarium poae gene sequence genetic variability Italy nonhuman priority journal seed kernel wheat Amplified Fragment Length Polymorphism Analysis Cereals Chromatography, High Pressure Liquid Depsipeptides Food Contamination Fungal Proteins Fusarium Genes, Fungal Genetic Variation Italy Limit of Detection Mycological Typing Techniques Mycotoxins Peptide Elongation Factor 1 Phylogeny Plant Diseases Polymerase Chain Reaction Species Specificity Tandem Mass Spectrometry Transcriptional Elongation Factors Trichothecenes Triticum Fungi Fusarium Fusarium poae Triticum aestivum Triticum turgidum subsp. durum The importance and widespread incidence of Fusarium poae as a natural contaminant of wheat in different climatic areas warrants investigation into the genetic diversity and toxin profile of a northern Italy population. Eighty-one strains of F. poae isolated from durum wheat kernels, identified by species-specific polymerase chain reaction and translation elongation factor-1α gene sequence analysis, were genetically characterized by the amplified fragment length polymorphism (AFLP) technique and analysed by high-pressure liquid chromatography for their ability to produce the beauvericin (BEA) and trichothecene mycotoxins. A high level of variability was observed by using AFLP analyses, with the lowest level of genetic similarity among the strains being approximately 61%. Most of the strains, 95%, produced BEA at <2655 μg g-1; 88% produced the trichothecene nivalenol at <865 μgg-1 and 76% produced the trichothecene fusarenon-X at <167 μg g-1. These data show that F. poae can produce high amounts of BEA together with trichothecenes, and can represent a high potential mycotoxin risk in Italy for wheat colonized by this species. © 2010 Taylor & Francis. Fil:Alvarez, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19440049_v27_n5_p729_Somma http://hdl.handle.net/20.500.12110/paper_19440049_v27_n5_p729_Somma |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fungi Fusarium Liquid chromatography/mass spectrometry (LC/MS) Mycology Mycotoxins Plants Polymerase chain reaction (PCR) Trichothecenes beauvericin elongation factor 1alpha fusarenon X trichothecene article controlled study food contamination fungal colonization fungal contamination fungal gene fungal genetics fungal plant disease fungal strain fungus isolation Fusarium Fusarium poae gene sequence genetic variability Italy nonhuman priority journal seed kernel wheat Amplified Fragment Length Polymorphism Analysis Cereals Chromatography, High Pressure Liquid Depsipeptides Food Contamination Fungal Proteins Fusarium Genes, Fungal Genetic Variation Italy Limit of Detection Mycological Typing Techniques Mycotoxins Peptide Elongation Factor 1 Phylogeny Plant Diseases Polymerase Chain Reaction Species Specificity Tandem Mass Spectrometry Transcriptional Elongation Factors Trichothecenes Triticum Fungi Fusarium Fusarium poae Triticum aestivum Triticum turgidum subsp. durum |
| spellingShingle |
Fungi Fusarium Liquid chromatography/mass spectrometry (LC/MS) Mycology Mycotoxins Plants Polymerase chain reaction (PCR) Trichothecenes beauvericin elongation factor 1alpha fusarenon X trichothecene article controlled study food contamination fungal colonization fungal contamination fungal gene fungal genetics fungal plant disease fungal strain fungus isolation Fusarium Fusarium poae gene sequence genetic variability Italy nonhuman priority journal seed kernel wheat Amplified Fragment Length Polymorphism Analysis Cereals Chromatography, High Pressure Liquid Depsipeptides Food Contamination Fungal Proteins Fusarium Genes, Fungal Genetic Variation Italy Limit of Detection Mycological Typing Techniques Mycotoxins Peptide Elongation Factor 1 Phylogeny Plant Diseases Polymerase Chain Reaction Species Specificity Tandem Mass Spectrometry Transcriptional Elongation Factors Trichothecenes Triticum Fungi Fusarium Fusarium poae Triticum aestivum Triticum turgidum subsp. durum Alvarez, Cora Lilia Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy |
| topic_facet |
Fungi Fusarium Liquid chromatography/mass spectrometry (LC/MS) Mycology Mycotoxins Plants Polymerase chain reaction (PCR) Trichothecenes beauvericin elongation factor 1alpha fusarenon X trichothecene article controlled study food contamination fungal colonization fungal contamination fungal gene fungal genetics fungal plant disease fungal strain fungus isolation Fusarium Fusarium poae gene sequence genetic variability Italy nonhuman priority journal seed kernel wheat Amplified Fragment Length Polymorphism Analysis Cereals Chromatography, High Pressure Liquid Depsipeptides Food Contamination Fungal Proteins Fusarium Genes, Fungal Genetic Variation Italy Limit of Detection Mycological Typing Techniques Mycotoxins Peptide Elongation Factor 1 Phylogeny Plant Diseases Polymerase Chain Reaction Species Specificity Tandem Mass Spectrometry Transcriptional Elongation Factors Trichothecenes Triticum Fungi Fusarium Fusarium poae Triticum aestivum Triticum turgidum subsp. durum |
| description |
The importance and widespread incidence of Fusarium poae as a natural contaminant of wheat in different climatic areas warrants investigation into the genetic diversity and toxin profile of a northern Italy population. Eighty-one strains of F. poae isolated from durum wheat kernels, identified by species-specific polymerase chain reaction and translation elongation factor-1α gene sequence analysis, were genetically characterized by the amplified fragment length polymorphism (AFLP) technique and analysed by high-pressure liquid chromatography for their ability to produce the beauvericin (BEA) and trichothecene mycotoxins. A high level of variability was observed by using AFLP analyses, with the lowest level of genetic similarity among the strains being approximately 61%. Most of the strains, 95%, produced BEA at <2655 μg g-1; 88% produced the trichothecene nivalenol at <865 μgg-1 and 76% produced the trichothecene fusarenon-X at <167 μg g-1. These data show that F. poae can produce high amounts of BEA together with trichothecenes, and can represent a high potential mycotoxin risk in Italy for wheat colonized by this species. © 2010 Taylor & Francis. |
| author |
Alvarez, Cora Lilia |
| author_facet |
Alvarez, Cora Lilia |
| author_sort |
Alvarez, Cora Lilia |
| title |
Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy |
| title_short |
Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy |
| title_full |
Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy |
| title_fullStr |
Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy |
| title_full_unstemmed |
Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy |
| title_sort |
trichothecene and beauvericin mycotoxin production and genetic variability in fusarium poae isolated from wheat kernels from northern italy |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19440049_v27_n5_p729_Somma http://hdl.handle.net/20.500.12110/paper_19440049_v27_n5_p729_Somma |
| work_keys_str_mv |
AT alvarezcoralilia trichotheceneandbeauvericinmycotoxinproductionandgeneticvariabilityinfusariumpoaeisolatedfromwheatkernelsfromnorthernitaly |
| _version_ |
1768542810938540032 |