Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance

The internal transcribed spacer region (ITS1 and ITS2) of the 18S-25S nuclear ribosomal DNA sequence and the intervening 5.8S region from five species of the genus Oxalis was amplified by polymerase chain reaction and subjected to direct DN A sequencing. On the basis of cytogenetic studies some spec...

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Publicado: 1996
Materias:
ITS
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19401736_v6_n6_p361_Tosto
http://hdl.handle.net/20.500.12110/paper_19401736_v6_n6_p361_Tosto
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spelling paper:paper_19401736_v6_n6_p361_Tosto2023-06-08T16:32:18Z Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance Andean crops ITS Oxalis rDNA plant DNA ribosome DNA article genetic transcription genetics molecular genetics nucleotide sequence plant sequence homology Base Sequence DNA, Plant DNA, Ribosomal Molecular Sequence Data Plants Sequence Homology, Nucleic Acid Transcription, Genetic Oxalis Oxalis tuberosa Tuberosa The internal transcribed spacer region (ITS1 and ITS2) of the 18S-25S nuclear ribosomal DNA sequence and the intervening 5.8S region from five species of the genus Oxalis was amplified by polymerase chain reaction and subjected to direct DN A sequencing. On the basis of cytogenetic studies some species of this genus were postulated to be related by the number of chromosomes. Sequence homologies in the ITS1, 5.8S and ITS2 among species are in good agreement with previous relationships established on the basis of chromosome numbers. We also indemnified a highly conserved sequence of six bp in the ITS1, reported to be present in a wide range of flowering plants, but not in the Oxalidaceae family to which the genus Oxalis belongs to. © 1996 Informa UK Ltd All rights reserved. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19401736_v6_n6_p361_Tosto http://hdl.handle.net/20.500.12110/paper_19401736_v6_n6_p361_Tosto
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Andean crops
ITS
Oxalis
rDNA
plant DNA
ribosome DNA
article
genetic transcription
genetics
molecular genetics
nucleotide sequence
plant
sequence homology
Base Sequence
DNA, Plant
DNA, Ribosomal
Molecular Sequence Data
Plants
Sequence Homology, Nucleic Acid
Transcription, Genetic
Oxalis
Oxalis tuberosa
Tuberosa
spellingShingle Andean crops
ITS
Oxalis
rDNA
plant DNA
ribosome DNA
article
genetic transcription
genetics
molecular genetics
nucleotide sequence
plant
sequence homology
Base Sequence
DNA, Plant
DNA, Ribosomal
Molecular Sequence Data
Plants
Sequence Homology, Nucleic Acid
Transcription, Genetic
Oxalis
Oxalis tuberosa
Tuberosa
Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance
topic_facet Andean crops
ITS
Oxalis
rDNA
plant DNA
ribosome DNA
article
genetic transcription
genetics
molecular genetics
nucleotide sequence
plant
sequence homology
Base Sequence
DNA, Plant
DNA, Ribosomal
Molecular Sequence Data
Plants
Sequence Homology, Nucleic Acid
Transcription, Genetic
Oxalis
Oxalis tuberosa
Tuberosa
description The internal transcribed spacer region (ITS1 and ITS2) of the 18S-25S nuclear ribosomal DNA sequence and the intervening 5.8S region from five species of the genus Oxalis was amplified by polymerase chain reaction and subjected to direct DN A sequencing. On the basis of cytogenetic studies some species of this genus were postulated to be related by the number of chromosomes. Sequence homologies in the ITS1, 5.8S and ITS2 among species are in good agreement with previous relationships established on the basis of chromosome numbers. We also indemnified a highly conserved sequence of six bp in the ITS1, reported to be present in a wide range of flowering plants, but not in the Oxalidaceae family to which the genus Oxalis belongs to. © 1996 Informa UK Ltd All rights reserved.
title Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance
title_short Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance
title_full Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance
title_fullStr Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance
title_full_unstemmed Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance
title_sort sequence analysis of the 5.8s ribosomal dna and internal transcribed spacers (its1 and its2) from five species of the oxalis tuberosa alliance
publishDate 1996
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19401736_v6_n6_p361_Tosto
http://hdl.handle.net/20.500.12110/paper_19401736_v6_n6_p361_Tosto
_version_ 1768545622977150976