The TATA-less promoter of VP1, a plant gene controlling seed germination
Vp1 is a seed-specific gene involved in the control of dormancy and germination. We here present the complete sequence of the sorghum vp1 promoter/enhancer region highlighting its main features, especially the lack of canonical TATA and CAAT boxes and the presence of elements responsive to abscisic...
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2001
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19401736_v12_n2_p107_Carrari http://hdl.handle.net/20.500.12110/paper_19401736_v12_n2_p107_Carrari |
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paper:paper_19401736_v12_n2_p107_Carrari2023-06-08T16:32:18Z The TATA-less promoter of VP1, a plant gene controlling seed germination Carrari, Fernando O. Frankel, Nicolás Lijavetzky, Diego C. Iusem, Norberto Daniel germination MITE Sorghum bicolor TATA-less promoter vpl gene Base Sequence Cereals Germination Molecular Sequence Data Plant Proteins Promoter Regions (Genetics) TATA Box Trans-Activators Transcription Initiation Site Acari Arabidopsis Embryophyta Sorghum bicolor Zea mays Vp1 is a seed-specific gene involved in the control of dormancy and germination. We here present the complete sequence of the sorghum vp1 promoter/enhancer region highlighting its main features, especially the lack of canonical TATA and CAAT boxes and the presence of elements responsive to abscisic acid and light. The region closest to the start of transcription is highly homologous to the partial proximal sequence reported for the maize vp1 promoter. This region is interrupted by a 57-nt stretch containing 14 CT microsatellite repeats. We observed a poor overall homology to the promoter from abi3 gene, the Arabidopsis counterpart bearing a similar coding sequence. However, there exists a high degree of homology (89%) between a TATA-rich 103-bp stretch of the sorghum vp1 promoter located about 700 nt upstream of the startpoint and miniature inverted transposable elements (MITEs) interspersed within the sorghum seed-specific kafirin cluster. This sorghum MITE-like element displays considerable homology (68%) to the TATA-less promoter from the sorghum NADP-malate dehydrogenase gene and lesser similarity to the Tourist, Pilgrim and Batuta MITEs previously identified within the promoter from the maize Abp1 (auxin-binding protein) gene. © 2001 OPA (Overseas Publishen Aswciatinn) N V. Fil:Carrari, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Frankel, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lijavetzky, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19401736_v12_n2_p107_Carrari http://hdl.handle.net/20.500.12110/paper_19401736_v12_n2_p107_Carrari |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
germination MITE Sorghum bicolor TATA-less promoter vpl gene Base Sequence Cereals Germination Molecular Sequence Data Plant Proteins Promoter Regions (Genetics) TATA Box Trans-Activators Transcription Initiation Site Acari Arabidopsis Embryophyta Sorghum bicolor Zea mays |
spellingShingle |
germination MITE Sorghum bicolor TATA-less promoter vpl gene Base Sequence Cereals Germination Molecular Sequence Data Plant Proteins Promoter Regions (Genetics) TATA Box Trans-Activators Transcription Initiation Site Acari Arabidopsis Embryophyta Sorghum bicolor Zea mays Carrari, Fernando O. Frankel, Nicolás Lijavetzky, Diego C. Iusem, Norberto Daniel The TATA-less promoter of VP1, a plant gene controlling seed germination |
topic_facet |
germination MITE Sorghum bicolor TATA-less promoter vpl gene Base Sequence Cereals Germination Molecular Sequence Data Plant Proteins Promoter Regions (Genetics) TATA Box Trans-Activators Transcription Initiation Site Acari Arabidopsis Embryophyta Sorghum bicolor Zea mays |
description |
Vp1 is a seed-specific gene involved in the control of dormancy and germination. We here present the complete sequence of the sorghum vp1 promoter/enhancer region highlighting its main features, especially the lack of canonical TATA and CAAT boxes and the presence of elements responsive to abscisic acid and light. The region closest to the start of transcription is highly homologous to the partial proximal sequence reported for the maize vp1 promoter. This region is interrupted by a 57-nt stretch containing 14 CT microsatellite repeats. We observed a poor overall homology to the promoter from abi3 gene, the Arabidopsis counterpart bearing a similar coding sequence. However, there exists a high degree of homology (89%) between a TATA-rich 103-bp stretch of the sorghum vp1 promoter located about 700 nt upstream of the startpoint and miniature inverted transposable elements (MITEs) interspersed within the sorghum seed-specific kafirin cluster. This sorghum MITE-like element displays considerable homology (68%) to the TATA-less promoter from the sorghum NADP-malate dehydrogenase gene and lesser similarity to the Tourist, Pilgrim and Batuta MITEs previously identified within the promoter from the maize Abp1 (auxin-binding protein) gene. © 2001 OPA (Overseas Publishen Aswciatinn) N V. |
author |
Carrari, Fernando O. Frankel, Nicolás Lijavetzky, Diego C. Iusem, Norberto Daniel |
author_facet |
Carrari, Fernando O. Frankel, Nicolás Lijavetzky, Diego C. Iusem, Norberto Daniel |
author_sort |
Carrari, Fernando O. |
title |
The TATA-less promoter of VP1, a plant gene controlling seed germination |
title_short |
The TATA-less promoter of VP1, a plant gene controlling seed germination |
title_full |
The TATA-less promoter of VP1, a plant gene controlling seed germination |
title_fullStr |
The TATA-less promoter of VP1, a plant gene controlling seed germination |
title_full_unstemmed |
The TATA-less promoter of VP1, a plant gene controlling seed germination |
title_sort |
tata-less promoter of vp1, a plant gene controlling seed germination |
publishDate |
2001 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19401736_v12_n2_p107_Carrari http://hdl.handle.net/20.500.12110/paper_19401736_v12_n2_p107_Carrari |
work_keys_str_mv |
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1768546089564110848 |