Recording gamma band oscillations in pedunculopontine nucleus neurons

Synaptic efferents from the PPN are known to modulate the neuronal activity of several intralaminar thalamic regions (e.g., the centrolateral/ parafascicular; Cl/Pf nucleus). The activation of either the PPN or Cl/Pf nuclei in vivo has been described to induce the arousal of the animal and an increm...

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Publicado:	2016
Materias:	Arousal Ca2+channels Current ramps Gamma oscillations Issue 115 N-Type Neuroscience P/Q-type calcium channel animal membrane potential nerve cell pedunculopontine tegmental nucleus rat Sprague Dawley rat Animals Calcium Channels Membrane Potentials Neurons Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1940087X_v2016_n115_p_Urbano http://hdl.handle.net/20.500.12110/paper_1940087X_v2016_n115_p_Urbano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_1940087X_v2016_n115_p_Urbano
record_format	dspace
spelling	paper:paper_1940087X_v2016_n115_p_Urbano2023-06-08T16:32:18Z Recording gamma band oscillations in pedunculopontine nucleus neurons Arousal Ca2+channels Current ramps Gamma oscillations Issue 115 N-Type Neuroscience P/Q-type calcium channel animal membrane potential nerve cell pedunculopontine tegmental nucleus rat Sprague Dawley rat Animals Calcium Channels Membrane Potentials Neurons Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley Synaptic efferents from the PPN are known to modulate the neuronal activity of several intralaminar thalamic regions (e.g., the centrolateral/ parafascicular; Cl/Pf nucleus). The activation of either the PPN or Cl/Pf nuclei in vivo has been described to induce the arousal of the animal and an increment in gamma band activity in the cortical electroencephalogram (EEG). The cellular mechanisms for the generation of gamma band oscillations in Reticular Activating System (RAS) neurons are the same as those found to generate gamma band oscillations in other brains nuclei. During current-clamp recordings of PPN neurons (from parasagittal slices from 9 - 25 day-old rats), the use of depolarizing square steps rapidly activated voltage-dependent potassium channels that prevented PPN neurons from being depolarized beyond -25 mV. Injecting 1 - 2 sec long depolarizing current ramps gradually depolarized PPN membrane potential resting values towards 0 mV. However, injecting depolarizing square pulses generated gamma-band oscillations of membrane potential that showed to be smaller in amplitude compared to the oscillations generated by ramps. All experiments were performed in the presence of voltage-gated sodium channels and fast synaptic receptors blockers. It has been shown that the activation of high-threshold voltage-dependent calcium channels underlie gamma-band oscillatory activity in PPN neurons. Specific methodological and pharmacological interventions are described here, providing the necessary tools to induce and sustain PPN subthreshold gamma band oscillation in vitro. © 2016 Journal of Visualized Experiments. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1940087X_v2016_n115_p_Urbano http://hdl.handle.net/20.500.12110/paper_1940087X_v2016_n115_p_Urbano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arousal Ca2+channels Current ramps Gamma oscillations Issue 115 N-Type Neuroscience P/Q-type calcium channel animal membrane potential nerve cell pedunculopontine tegmental nucleus rat Sprague Dawley rat Animals Calcium Channels Membrane Potentials Neurons Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley
spellingShingle	Arousal Ca2+channels Current ramps Gamma oscillations Issue 115 N-Type Neuroscience P/Q-type calcium channel animal membrane potential nerve cell pedunculopontine tegmental nucleus rat Sprague Dawley rat Animals Calcium Channels Membrane Potentials Neurons Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley Recording gamma band oscillations in pedunculopontine nucleus neurons
topic_facet	Arousal Ca2+channels Current ramps Gamma oscillations Issue 115 N-Type Neuroscience P/Q-type calcium channel animal membrane potential nerve cell pedunculopontine tegmental nucleus rat Sprague Dawley rat Animals Calcium Channels Membrane Potentials Neurons Pedunculopontine Tegmental Nucleus Rats Rats, Sprague-Dawley
description	Synaptic efferents from the PPN are known to modulate the neuronal activity of several intralaminar thalamic regions (e.g., the centrolateral/ parafascicular; Cl/Pf nucleus). The activation of either the PPN or Cl/Pf nuclei in vivo has been described to induce the arousal of the animal and an increment in gamma band activity in the cortical electroencephalogram (EEG). The cellular mechanisms for the generation of gamma band oscillations in Reticular Activating System (RAS) neurons are the same as those found to generate gamma band oscillations in other brains nuclei. During current-clamp recordings of PPN neurons (from parasagittal slices from 9 - 25 day-old rats), the use of depolarizing square steps rapidly activated voltage-dependent potassium channels that prevented PPN neurons from being depolarized beyond -25 mV. Injecting 1 - 2 sec long depolarizing current ramps gradually depolarized PPN membrane potential resting values towards 0 mV. However, injecting depolarizing square pulses generated gamma-band oscillations of membrane potential that showed to be smaller in amplitude compared to the oscillations generated by ramps. All experiments were performed in the presence of voltage-gated sodium channels and fast synaptic receptors blockers. It has been shown that the activation of high-threshold voltage-dependent calcium channels underlie gamma-band oscillatory activity in PPN neurons. Specific methodological and pharmacological interventions are described here, providing the necessary tools to induce and sustain PPN subthreshold gamma band oscillation in vitro. © 2016 Journal of Visualized Experiments.
title	Recording gamma band oscillations in pedunculopontine nucleus neurons
title_short	Recording gamma band oscillations in pedunculopontine nucleus neurons
title_full	Recording gamma band oscillations in pedunculopontine nucleus neurons
title_fullStr	Recording gamma band oscillations in pedunculopontine nucleus neurons
title_full_unstemmed	Recording gamma band oscillations in pedunculopontine nucleus neurons
title_sort	recording gamma band oscillations in pedunculopontine nucleus neurons
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1940087X_v2016_n115_p_Urbano http://hdl.handle.net/20.500.12110/paper_1940087X_v2016_n115_p_Urbano
_version_	1768544104484962304

Recording gamma band oscillations in pedunculopontine nucleus neurons

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