Interactions of gold nanoparticles with a phospholipid monolayer membrane on mercury
It is demonstrated that a compact monolayer of 1,2-dioleoyl-sn-glycero-3- phosphocholine adsorbed to a hanging mercury drop electrode can serve as a simple electrochemical model system to study biomembrane penetration by gold nanoparticles. The hydrogen redox-chemistry characteristic of ligand-stabi...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19360851_v8_n6_p6074_Gordillo http://hdl.handle.net/20.500.12110/paper_19360851_v8_n6_p6074_Gordillo |
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paper:paper_19360851_v8_n6_p6074_Gordillo2023-06-08T16:32:09Z Interactions of gold nanoparticles with a phospholipid monolayer membrane on mercury electrochemistry gold nanoparticles membrane penetration mercury electrode nanotoxicology phospholipid monolayer Cell culture Electrochemistry Electrodes Equipment testing Gold Mercury (metal) Monolayers Phospholipids Gold Nanoparticles Membrane penetration Mercury electrodes Nanotoxicology Phospholipid monolayers Metal nanoparticles 1,2-oleoylphosphatidylcholine artificial membrane gold hydrogen ligand mercury metal nanoparticle phosphatidylcholine phospholipid adsorption artificial membrane chemistry electrochemistry electrode fibroblast HeLa cell line human metabolism nanotechnology oxidation reduction reaction procedures Adsorption Electrochemistry Electrodes Fibroblasts Gold HeLa Cells Humans Hydrogen Ligands Membranes, Artificial Mercury Metal Nanoparticles Nanotechnology Oxidation-Reduction Phosphatidylcholines Phospholipids It is demonstrated that a compact monolayer of 1,2-dioleoyl-sn-glycero-3- phosphocholine adsorbed to a hanging mercury drop electrode can serve as a simple electrochemical model system to study biomembrane penetration by gold nanoparticles. The hydrogen redox-chemistry characteristic of ligand-stabilized gold nanoparticles in molecularly close contact with a mercury electrode is used as an indicator of membrane penetration. Results for water-dispersible gold nanoparticles of two different sizes are reported, and comparisons are made with the cellular uptake of the same preparations of nanoparticles by a common human fibroblast cell line. The experimental system described here can be used to study physicochemical aspects of membrane penetration in the absence of complex biological mechanisms, and it could also be a starting point for the development of a test bed for the toxicity of nanomaterials. © 2014 American Chemical Society. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19360851_v8_n6_p6074_Gordillo http://hdl.handle.net/20.500.12110/paper_19360851_v8_n6_p6074_Gordillo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
electrochemistry gold nanoparticles membrane penetration mercury electrode nanotoxicology phospholipid monolayer Cell culture Electrochemistry Electrodes Equipment testing Gold Mercury (metal) Monolayers Phospholipids Gold Nanoparticles Membrane penetration Mercury electrodes Nanotoxicology Phospholipid monolayers Metal nanoparticles 1,2-oleoylphosphatidylcholine artificial membrane gold hydrogen ligand mercury metal nanoparticle phosphatidylcholine phospholipid adsorption artificial membrane chemistry electrochemistry electrode fibroblast HeLa cell line human metabolism nanotechnology oxidation reduction reaction procedures Adsorption Electrochemistry Electrodes Fibroblasts Gold HeLa Cells Humans Hydrogen Ligands Membranes, Artificial Mercury Metal Nanoparticles Nanotechnology Oxidation-Reduction Phosphatidylcholines Phospholipids |
| spellingShingle |
electrochemistry gold nanoparticles membrane penetration mercury electrode nanotoxicology phospholipid monolayer Cell culture Electrochemistry Electrodes Equipment testing Gold Mercury (metal) Monolayers Phospholipids Gold Nanoparticles Membrane penetration Mercury electrodes Nanotoxicology Phospholipid monolayers Metal nanoparticles 1,2-oleoylphosphatidylcholine artificial membrane gold hydrogen ligand mercury metal nanoparticle phosphatidylcholine phospholipid adsorption artificial membrane chemistry electrochemistry electrode fibroblast HeLa cell line human metabolism nanotechnology oxidation reduction reaction procedures Adsorption Electrochemistry Electrodes Fibroblasts Gold HeLa Cells Humans Hydrogen Ligands Membranes, Artificial Mercury Metal Nanoparticles Nanotechnology Oxidation-Reduction Phosphatidylcholines Phospholipids Interactions of gold nanoparticles with a phospholipid monolayer membrane on mercury |
| topic_facet |
electrochemistry gold nanoparticles membrane penetration mercury electrode nanotoxicology phospholipid monolayer Cell culture Electrochemistry Electrodes Equipment testing Gold Mercury (metal) Monolayers Phospholipids Gold Nanoparticles Membrane penetration Mercury electrodes Nanotoxicology Phospholipid monolayers Metal nanoparticles 1,2-oleoylphosphatidylcholine artificial membrane gold hydrogen ligand mercury metal nanoparticle phosphatidylcholine phospholipid adsorption artificial membrane chemistry electrochemistry electrode fibroblast HeLa cell line human metabolism nanotechnology oxidation reduction reaction procedures Adsorption Electrochemistry Electrodes Fibroblasts Gold HeLa Cells Humans Hydrogen Ligands Membranes, Artificial Mercury Metal Nanoparticles Nanotechnology Oxidation-Reduction Phosphatidylcholines Phospholipids |
| description |
It is demonstrated that a compact monolayer of 1,2-dioleoyl-sn-glycero-3- phosphocholine adsorbed to a hanging mercury drop electrode can serve as a simple electrochemical model system to study biomembrane penetration by gold nanoparticles. The hydrogen redox-chemistry characteristic of ligand-stabilized gold nanoparticles in molecularly close contact with a mercury electrode is used as an indicator of membrane penetration. Results for water-dispersible gold nanoparticles of two different sizes are reported, and comparisons are made with the cellular uptake of the same preparations of nanoparticles by a common human fibroblast cell line. The experimental system described here can be used to study physicochemical aspects of membrane penetration in the absence of complex biological mechanisms, and it could also be a starting point for the development of a test bed for the toxicity of nanomaterials. © 2014 American Chemical Society. |
| title |
Interactions of gold nanoparticles with a phospholipid monolayer membrane on mercury |
| title_short |
Interactions of gold nanoparticles with a phospholipid monolayer membrane on mercury |
| title_full |
Interactions of gold nanoparticles with a phospholipid monolayer membrane on mercury |
| title_fullStr |
Interactions of gold nanoparticles with a phospholipid monolayer membrane on mercury |
| title_full_unstemmed |
Interactions of gold nanoparticles with a phospholipid monolayer membrane on mercury |
| title_sort |
interactions of gold nanoparticles with a phospholipid monolayer membrane on mercury |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19360851_v8_n6_p6074_Gordillo http://hdl.handle.net/20.500.12110/paper_19360851_v8_n6_p6074_Gordillo |
| _version_ |
1768542288915464192 |