Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.)
Cultivated sunflower (Helianthus annuus L.), an important source of edible vegetable oil, shows rapid onset of senescence, which limits production by reducing photosynthetic capacity under specific growing conditions. Carbon for grain filling depends strongly on light interception by green leaf area...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v9_n8_p_Moschen http://hdl.handle.net/20.500.12110/paper_19326203_v9_n8_p_Moschen |
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paper:paper_19326203_v9_n8_p_Moschen2023-06-08T16:31:25Z Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) Hopp, Horacio Esteban Fernández, Paula Virginia Heinz, Ruth Amelia chlorophyll nitrogen transcription factor carbohydrate metabolism gene expression profiling genetics genomics growth, development and aging metabolism photosynthesis plant leaf solubility sunflower time Carbohydrate Metabolism Chlorophyll Gene Expression Profiling Genomics Helianthus Nitrogen Photosynthesis Plant Leaves Solubility Time Factors Transcription Factors Cultivated sunflower (Helianthus annuus L.), an important source of edible vegetable oil, shows rapid onset of senescence, which limits production by reducing photosynthetic capacity under specific growing conditions. Carbon for grain filling depends strongly on light interception by green leaf area, which diminishes during grain filling due to leaf senescence. Transcription factors (TFs) regulate the progression of leaf senescence in plants and have been well explored in model systems, but information for many agronomic crops remains limited. Here, we characterize the expression profiles of a set of putative senescence associated genes (SAGs) identified by a candidate gene approach and sunflower microarray expression studies. We examined a time course of sunflower leaves undergoing natural senescence and used quantitative PCR (qPCR) to measure the expression of 11 candidate genes representing the NAC, WRKY, MYB and NF-Y TF families. In addition, we measured physiological parameters such as chlorophyll, total soluble sugars and nitrogen content. The expression of Ha-NAC01, Ha-NAC03, Ha-NAC04, Ha-NAC05 and Ha-MYB01 TFs increased before the remobilization rate increased and therefore, before the appearance of the first physiological symptoms of senescence, whereas Ha-NAC02 expression decreased. In addition, we also examined the trifurcate feed-forward pathway (involving ORE1, miR164, and ETHYLENE INSENSITIVE 2) previously reported for Arabidopsis. We measured transcription of Ha-NAC01 (the sunflower homolog of ORE1) and Ha-EIN2, along with the levels of miR164, in two leaves from different stem positions, and identified differences in transcription between basal and upper leaves. Interestingly, Ha-NAC01 and Ha-EIN2 transcription profiles showed an earlier up-regulation in upper leaves of plants close to maturity, compared with basal leaves of plants at pre-anthesis stages. These results suggest that the H. annuus TFs characterized in this work could play important roles as potential triggers of leaf senescence and thus can be considered putative candidate genes for senescence in sunflower. © 2014 Moschen et al. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernandez, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Heinz, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v9_n8_p_Moschen http://hdl.handle.net/20.500.12110/paper_19326203_v9_n8_p_Moschen |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
chlorophyll nitrogen transcription factor carbohydrate metabolism gene expression profiling genetics genomics growth, development and aging metabolism photosynthesis plant leaf solubility sunflower time Carbohydrate Metabolism Chlorophyll Gene Expression Profiling Genomics Helianthus Nitrogen Photosynthesis Plant Leaves Solubility Time Factors Transcription Factors |
| spellingShingle |
chlorophyll nitrogen transcription factor carbohydrate metabolism gene expression profiling genetics genomics growth, development and aging metabolism photosynthesis plant leaf solubility sunflower time Carbohydrate Metabolism Chlorophyll Gene Expression Profiling Genomics Helianthus Nitrogen Photosynthesis Plant Leaves Solubility Time Factors Transcription Factors Hopp, Horacio Esteban Fernández, Paula Virginia Heinz, Ruth Amelia Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| topic_facet |
chlorophyll nitrogen transcription factor carbohydrate metabolism gene expression profiling genetics genomics growth, development and aging metabolism photosynthesis plant leaf solubility sunflower time Carbohydrate Metabolism Chlorophyll Gene Expression Profiling Genomics Helianthus Nitrogen Photosynthesis Plant Leaves Solubility Time Factors Transcription Factors |
| description |
Cultivated sunflower (Helianthus annuus L.), an important source of edible vegetable oil, shows rapid onset of senescence, which limits production by reducing photosynthetic capacity under specific growing conditions. Carbon for grain filling depends strongly on light interception by green leaf area, which diminishes during grain filling due to leaf senescence. Transcription factors (TFs) regulate the progression of leaf senescence in plants and have been well explored in model systems, but information for many agronomic crops remains limited. Here, we characterize the expression profiles of a set of putative senescence associated genes (SAGs) identified by a candidate gene approach and sunflower microarray expression studies. We examined a time course of sunflower leaves undergoing natural senescence and used quantitative PCR (qPCR) to measure the expression of 11 candidate genes representing the NAC, WRKY, MYB and NF-Y TF families. In addition, we measured physiological parameters such as chlorophyll, total soluble sugars and nitrogen content. The expression of Ha-NAC01, Ha-NAC03, Ha-NAC04, Ha-NAC05 and Ha-MYB01 TFs increased before the remobilization rate increased and therefore, before the appearance of the first physiological symptoms of senescence, whereas Ha-NAC02 expression decreased. In addition, we also examined the trifurcate feed-forward pathway (involving ORE1, miR164, and ETHYLENE INSENSITIVE 2) previously reported for Arabidopsis. We measured transcription of Ha-NAC01 (the sunflower homolog of ORE1) and Ha-EIN2, along with the levels of miR164, in two leaves from different stem positions, and identified differences in transcription between basal and upper leaves. Interestingly, Ha-NAC01 and Ha-EIN2 transcription profiles showed an earlier up-regulation in upper leaves of plants close to maturity, compared with basal leaves of plants at pre-anthesis stages. These results suggest that the H. annuus TFs characterized in this work could play important roles as potential triggers of leaf senescence and thus can be considered putative candidate genes for senescence in sunflower. © 2014 Moschen et al. |
| author |
Hopp, Horacio Esteban Fernández, Paula Virginia Heinz, Ruth Amelia |
| author_facet |
Hopp, Horacio Esteban Fernández, Paula Virginia Heinz, Ruth Amelia |
| author_sort |
Hopp, Horacio Esteban |
| title |
Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| title_short |
Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| title_full |
Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| title_fullStr |
Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| title_full_unstemmed |
Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.) |
| title_sort |
identification of candidate genes associated with leaf senescence in cultivated sunflower (helianthus annuus l.) |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v9_n8_p_Moschen http://hdl.handle.net/20.500.12110/paper_19326203_v9_n8_p_Moschen |
| work_keys_str_mv |
AT hopphoracioesteban identificationofcandidategenesassociatedwithleafsenescenceincultivatedsunflowerhelianthusannuusl AT fernandezpaulavirginia identificationofcandidategenesassociatedwithleafsenescenceincultivatedsunflowerhelianthusannuusl AT heinzruthamelia identificationofcandidategenesassociatedwithleafsenescenceincultivatedsunflowerhelianthusannuusl |
| _version_ |
1768544432533012480 |