High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes

Pseudomonas extremaustralis produces mainly polyhydroxybutyrate (PHB), a short chain length polyhydroxyalkanoate (sclPHA) infrequently found in Pseudomonas species. Previous studies with this strain demonstrated that PHB genes are located in a genomic island. In this work, the analysis of the genome...

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Autores principales:	Catone, Mariela Verónica, Ruiz, Jimena Alicia
Publicado:	2014
Materias:	bacterial protein Phal protein PhbP protein poly(3 hydroxybutyric acid) polyhydroxyalkanoate synthase unclassified drug acyltransferase hydroxybutyric acid poly(3-hydroxyalkanoic acid) synthase article bacterial gene bacterial genome bacterial growth bacterial metabolism Burkholderiales gene cluster gene expression gene insertion genetic code genetic complementation genetic organization genetic similarity genome analysis nonhuman open reading frame phaC1 gene phaC2 gene PhbC gene Pseudomonas Pseudomonas extremaustralis genetics horizontal gene transfer metabolism Acyltransferases Bacterial Proteins Gene Transfer, Horizontal Genes, Bacterial Hydroxybutyrates
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v9_n6_p_Catone http://hdl.handle.net/20.500.12110/paper_19326203_v9_n6_p_Catone
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_19326203_v9_n6_p_Catone2025-07-30T19:07:01Z High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes Catone, Mariela Verónica Ruiz, Jimena Alicia bacterial protein Phal protein PhbP protein poly(3 hydroxybutyric acid) polyhydroxyalkanoate synthase unclassified drug acyltransferase bacterial protein hydroxybutyric acid poly(3-hydroxyalkanoic acid) synthase article bacterial gene bacterial genome bacterial growth bacterial metabolism Burkholderiales gene cluster gene expression gene insertion genetic code genetic complementation genetic organization genetic similarity genome analysis nonhuman open reading frame phaC1 gene phaC2 gene PhbC gene Pseudomonas Pseudomonas extremaustralis genetics horizontal gene transfer metabolism Pseudomonas Acyltransferases Bacterial Proteins Gene Transfer, Horizontal Genes, Bacterial Hydroxybutyrates Pseudomonas Pseudomonas extremaustralis produces mainly polyhydroxybutyrate (PHB), a short chain length polyhydroxyalkanoate (sclPHA) infrequently found in Pseudomonas species. Previous studies with this strain demonstrated that PHB genes are located in a genomic island. In this work, the analysis of the genome of P. extremaustralis revealed the presence of another PHB cluster phbFPX, with high similarity to genes belonging to Burkholderiales, and also a cluster, phaC1ZC2D, coding for medium chain length PHA production (mclPHA). All mclPHA genes showed high similarity to genes from Pseudomonas species and interestingly, this cluster also showed a natural insertion of seven ORFs not related to mclPHA metabolism. Besides PHB, P. extremaustralis is able to produce mclPHA although in minor amounts. Complementation analysis demonstrated that both mclPHA synthases, PhaC1 and PhaC2, were functional. RT-qPCR analysis showed different levels of expression for the PHB synthase, phbC, and the mclPHA synthases. The expression level of phbC, was significantly higher than the obtained for phaC1 and phaC2, in late exponential phase cultures. The analysis of the proteins bound to the PHA granules showed the presence of PhbC and PhaC1, whilst PhaC2 could not be detected. In addition, two phasin like proteins (PhbP and PhaI) associated with the production of scl and mcl PHAs, respectively, were detected. The results of this work show the high efficiency of a foreign gene (phbC) in comparison with the mclPHA core genome genes (phaC1 and phaC2) indicating that the ability of P. extremaustralis to produce high amounts of PHB could be explained by the different expression levels of the genes encoding the scl and mcl PHA synthases. © 2014 Catone et al. Fil:Catone, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v9_n6_p_Catone http://hdl.handle.net/20.500.12110/paper_19326203_v9_n6_p_Catone
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bacterial protein Phal protein PhbP protein poly(3 hydroxybutyric acid) polyhydroxyalkanoate synthase unclassified drug acyltransferase bacterial protein hydroxybutyric acid poly(3-hydroxyalkanoic acid) synthase article bacterial gene bacterial genome bacterial growth bacterial metabolism Burkholderiales gene cluster gene expression gene insertion genetic code genetic complementation genetic organization genetic similarity genome analysis nonhuman open reading frame phaC1 gene phaC2 gene PhbC gene Pseudomonas Pseudomonas extremaustralis genetics horizontal gene transfer metabolism Pseudomonas Acyltransferases Bacterial Proteins Gene Transfer, Horizontal Genes, Bacterial Hydroxybutyrates Pseudomonas
spellingShingle	bacterial protein Phal protein PhbP protein poly(3 hydroxybutyric acid) polyhydroxyalkanoate synthase unclassified drug acyltransferase bacterial protein hydroxybutyric acid poly(3-hydroxyalkanoic acid) synthase article bacterial gene bacterial genome bacterial growth bacterial metabolism Burkholderiales gene cluster gene expression gene insertion genetic code genetic complementation genetic organization genetic similarity genome analysis nonhuman open reading frame phaC1 gene phaC2 gene PhbC gene Pseudomonas Pseudomonas extremaustralis genetics horizontal gene transfer metabolism Pseudomonas Acyltransferases Bacterial Proteins Gene Transfer, Horizontal Genes, Bacterial Hydroxybutyrates Pseudomonas Catone, Mariela Verónica Ruiz, Jimena Alicia High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes
topic_facet	bacterial protein Phal protein PhbP protein poly(3 hydroxybutyric acid) polyhydroxyalkanoate synthase unclassified drug acyltransferase bacterial protein hydroxybutyric acid poly(3-hydroxyalkanoic acid) synthase article bacterial gene bacterial genome bacterial growth bacterial metabolism Burkholderiales gene cluster gene expression gene insertion genetic code genetic complementation genetic organization genetic similarity genome analysis nonhuman open reading frame phaC1 gene phaC2 gene PhbC gene Pseudomonas Pseudomonas extremaustralis genetics horizontal gene transfer metabolism Pseudomonas Acyltransferases Bacterial Proteins Gene Transfer, Horizontal Genes, Bacterial Hydroxybutyrates Pseudomonas
description	Pseudomonas extremaustralis produces mainly polyhydroxybutyrate (PHB), a short chain length polyhydroxyalkanoate (sclPHA) infrequently found in Pseudomonas species. Previous studies with this strain demonstrated that PHB genes are located in a genomic island. In this work, the analysis of the genome of P. extremaustralis revealed the presence of another PHB cluster phbFPX, with high similarity to genes belonging to Burkholderiales, and also a cluster, phaC1ZC2D, coding for medium chain length PHA production (mclPHA). All mclPHA genes showed high similarity to genes from Pseudomonas species and interestingly, this cluster also showed a natural insertion of seven ORFs not related to mclPHA metabolism. Besides PHB, P. extremaustralis is able to produce mclPHA although in minor amounts. Complementation analysis demonstrated that both mclPHA synthases, PhaC1 and PhaC2, were functional. RT-qPCR analysis showed different levels of expression for the PHB synthase, phbC, and the mclPHA synthases. The expression level of phbC, was significantly higher than the obtained for phaC1 and phaC2, in late exponential phase cultures. The analysis of the proteins bound to the PHA granules showed the presence of PhbC and PhaC1, whilst PhaC2 could not be detected. In addition, two phasin like proteins (PhbP and PhaI) associated with the production of scl and mcl PHAs, respectively, were detected. The results of this work show the high efficiency of a foreign gene (phbC) in comparison with the mclPHA core genome genes (phaC1 and phaC2) indicating that the ability of P. extremaustralis to produce high amounts of PHB could be explained by the different expression levels of the genes encoding the scl and mcl PHA synthases. © 2014 Catone et al.
author	Catone, Mariela Verónica Ruiz, Jimena Alicia
author_facet	Catone, Mariela Verónica Ruiz, Jimena Alicia
author_sort	Catone, Mariela Verónica
title	High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes
title_short	High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes
title_full	High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes
title_fullStr	High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes
title_full_unstemmed	High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes
title_sort	high polyhydroxybutyrate production in pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v9_n6_p_Catone http://hdl.handle.net/20.500.12110/paper_19326203_v9_n6_p_Catone
work_keys_str_mv	AT catonemarielaveronica highpolyhydroxybutyrateproductioninpseudomonasextremaustralisisassociatedwithdifferentialexpressionofhorizontallyacquiredandcoregenomepolyhydroxyalkanoatesynthasegenes AT ruizjimenaalicia highpolyhydroxybutyrateproductioninpseudomonasextremaustralisisassociatedwithdifferentialexpressionofhorizontallyacquiredandcoregenomepolyhydroxyalkanoatesynthasegenes
_version_	1840320733148872704

High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes

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