The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site
It is generally accepted that the immediately releasable pool is a group of readily releasable vesicles that are closely associated with voltage dependent Ca2+ channels. We have previously shown that exocytosis of this pool is specifically coupled to P/Q Ca2+ current. Accordingly, in the present wor...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n1_p_Alvarez http://hdl.handle.net/20.500.12110/paper_19326203_v8_n1_p_Alvarez |
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paper:paper_19326203_v8_n1_p_Alvarez2023-06-08T16:31:13Z The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site Alvarez, Yanina Daniela Belingheri, Ana Veronica Perez Bay, Andrés Ezequiel Marengo, Fernando Diego calcium channel P type calcium channel Q type omega agatoxin IVA potassium ion animal cell article binding site calcium current cell interaction cell vacuole chromaffin cell controlled study depolarization exocytosis mouse nerve cell stimulation nonhuman protein expression protein interaction sequence analysis synapse transient expression Animals Calcium Calcium Channels, P-Type Calcium Channels, Q-Type Cells, Cultured Chromaffin Cells Exocytosis Mice Secretory Vesicles It is generally accepted that the immediately releasable pool is a group of readily releasable vesicles that are closely associated with voltage dependent Ca2+ channels. We have previously shown that exocytosis of this pool is specifically coupled to P/Q Ca2+ current. Accordingly, in the present work we found that the Ca2+ current flowing through P/Q-type Ca2+ channels is 8 times more effective at inducing exocytosis in response to short stimuli than the current carried by L-type channels. To investigate the mechanism that underlies the coupling between the immediately releasable pool and P/Q-type channels we transiently expressed in mouse chromaffin cells peptides corresponding to the synaptic protein interaction site of Cav2.2 to competitively uncouple P/Q-type channels from the secretory vesicle release complex. This treatment reduced the efficiency of Ca2+ current to induce exocytosis to similar values as direct inhibition of P/Q-type channels via ω-agatoxin-IVA. In addition, the same treatment markedly reduced immediately releasable pool exocytosis, but did not affect the exocytosis provoked by sustained electric or high K+ stimulation. Together, our results indicate that the synaptic protein interaction site is a crucial factor for the establishment of the functional coupling between immediately releasable pool vesicles and P/Q-type Ca2+ channels. © 2013 Álvarez et al. Fil:Álvarez, Y.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Belingheri, A.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Perez Bay, A.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marengo, F.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n1_p_Alvarez http://hdl.handle.net/20.500.12110/paper_19326203_v8_n1_p_Alvarez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
calcium channel P type calcium channel Q type omega agatoxin IVA potassium ion animal cell article binding site calcium current cell interaction cell vacuole chromaffin cell controlled study depolarization exocytosis mouse nerve cell stimulation nonhuman protein expression protein interaction sequence analysis synapse transient expression Animals Calcium Calcium Channels, P-Type Calcium Channels, Q-Type Cells, Cultured Chromaffin Cells Exocytosis Mice Secretory Vesicles |
| spellingShingle |
calcium channel P type calcium channel Q type omega agatoxin IVA potassium ion animal cell article binding site calcium current cell interaction cell vacuole chromaffin cell controlled study depolarization exocytosis mouse nerve cell stimulation nonhuman protein expression protein interaction sequence analysis synapse transient expression Animals Calcium Calcium Channels, P-Type Calcium Channels, Q-Type Cells, Cultured Chromaffin Cells Exocytosis Mice Secretory Vesicles Alvarez, Yanina Daniela Belingheri, Ana Veronica Perez Bay, Andrés Ezequiel Marengo, Fernando Diego The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site |
| topic_facet |
calcium channel P type calcium channel Q type omega agatoxin IVA potassium ion animal cell article binding site calcium current cell interaction cell vacuole chromaffin cell controlled study depolarization exocytosis mouse nerve cell stimulation nonhuman protein expression protein interaction sequence analysis synapse transient expression Animals Calcium Calcium Channels, P-Type Calcium Channels, Q-Type Cells, Cultured Chromaffin Cells Exocytosis Mice Secretory Vesicles |
| description |
It is generally accepted that the immediately releasable pool is a group of readily releasable vesicles that are closely associated with voltage dependent Ca2+ channels. We have previously shown that exocytosis of this pool is specifically coupled to P/Q Ca2+ current. Accordingly, in the present work we found that the Ca2+ current flowing through P/Q-type Ca2+ channels is 8 times more effective at inducing exocytosis in response to short stimuli than the current carried by L-type channels. To investigate the mechanism that underlies the coupling between the immediately releasable pool and P/Q-type channels we transiently expressed in mouse chromaffin cells peptides corresponding to the synaptic protein interaction site of Cav2.2 to competitively uncouple P/Q-type channels from the secretory vesicle release complex. This treatment reduced the efficiency of Ca2+ current to induce exocytosis to similar values as direct inhibition of P/Q-type channels via ω-agatoxin-IVA. In addition, the same treatment markedly reduced immediately releasable pool exocytosis, but did not affect the exocytosis provoked by sustained electric or high K+ stimulation. Together, our results indicate that the synaptic protein interaction site is a crucial factor for the establishment of the functional coupling between immediately releasable pool vesicles and P/Q-type Ca2+ channels. © 2013 Álvarez et al. |
| author |
Alvarez, Yanina Daniela Belingheri, Ana Veronica Perez Bay, Andrés Ezequiel Marengo, Fernando Diego |
| author_facet |
Alvarez, Yanina Daniela Belingheri, Ana Veronica Perez Bay, Andrés Ezequiel Marengo, Fernando Diego |
| author_sort |
Alvarez, Yanina Daniela |
| title |
The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site |
| title_short |
The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site |
| title_full |
The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site |
| title_fullStr |
The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site |
| title_full_unstemmed |
The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site |
| title_sort |
immediately releasable pool of mouse chromaffin cell vesicles is coupled to p/q-type calcium channels via the synaptic protein interaction site |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n1_p_Alvarez http://hdl.handle.net/20.500.12110/paper_19326203_v8_n1_p_Alvarez |
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