A first insight into pycnoporus sanguineus BAFC 2126 transcriptome
Fungi of the genus Pycnoporus are white-rot basidiomycetes widely studied because of their ability to synthesize high added-value compounds and enzymes of industrial interest. Here we report the sequencing, assembly and analysis of the transcriptome of Pycnoporus sanguineus BAFC 2126 grown at statio...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n12_p_Rohr http://hdl.handle.net/20.500.12110/paper_19326203_v8_n12_p_Rohr |
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paper:paper_19326203_v8_n12_p_Rohr2023-06-08T16:31:12Z A first insight into pycnoporus sanguineus BAFC 2126 transcriptome beta tubulin carbohydrate esterase copper radical oxidase cytochrome p450 oxygenase elongation factor 1alpha enzyme epoxide hydrolase glutathione transferase glyceraldehyde 3 phosphate dehydrogenase glycosidase GMC oxidoreductase laccase lignin peroxidase lignocellulose manganese peroxidase peroxidase polysaccharide lyase RNA polymerase II sesquiterpenoid transcriptome triterpenoid unclassified drug article controlled study culture medium degradation enzyme modification enzyme synthesis homeostasis hydrolysis nonhuman nucleotide sequence protein database protein domain Pycnoporus Pycnoporus sanguineus pyrosequencing RNA analysis RNA sequence sequence analysis xenobiotic metabolism Carbohydrate Metabolism Fungal Proteins Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Pycnoporus Transcriptome Fungi of the genus Pycnoporus are white-rot basidiomycetes widely studied because of their ability to synthesize high added-value compounds and enzymes of industrial interest. Here we report the sequencing, assembly and analysis of the transcriptome of Pycnoporus sanguineus BAFC 2126 grown at stationary phase, in media supplemented with copper sulfate. Using the 454 pyrosequencing platform we obtained a total of 226,336 reads (88,779,843 bases) that were filtered and de novo assembled to generate a reference transcriptome of 7,303 transcripts. Putative functions were assigned for 4,732 transcripts by searching similarities of six-frame translated sequences against a customized protein database and by the presence of conserved protein domains. Through the analysis of translated sequences we identified transcripts encoding 178 putative carbohydrate active enzymes, including representatives of 15 families with roles in lignocellulose degradation. Furthermore, we found many transcripts encoding enzymes related to lignin hydrolysis and modification, including laccases and peroxidases, as well as GMC oxidoreductases, copper radical oxidases and other enzymes involved in the generation of extracellular hydrogen peroxide and iron homeostasis. Finally, we identified the transcripts encoding all of the enzymes involved in terpenoid backbone biosynthesis pathway, various terpene synthases related to the biosynthesis of sesquiterpenoids and triterpenoids precursors, and also cytochrome P450 monooxygenases, glutathione S-transferases and epoxide hydrolases with potential functions in the biodegradation of xenobiotics and the enantioselective biosynthesis of biologically active drugs. To our knowledge this is the first report of a transcriptome of genus Pycnoporus and a resource for future molecular studies in P. sanguineus. Copyright: © 2013 Rohr et al. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n12_p_Rohr http://hdl.handle.net/20.500.12110/paper_19326203_v8_n12_p_Rohr |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
beta tubulin carbohydrate esterase copper radical oxidase cytochrome p450 oxygenase elongation factor 1alpha enzyme epoxide hydrolase glutathione transferase glyceraldehyde 3 phosphate dehydrogenase glycosidase GMC oxidoreductase laccase lignin peroxidase lignocellulose manganese peroxidase peroxidase polysaccharide lyase RNA polymerase II sesquiterpenoid transcriptome triterpenoid unclassified drug article controlled study culture medium degradation enzyme modification enzyme synthesis homeostasis hydrolysis nonhuman nucleotide sequence protein database protein domain Pycnoporus Pycnoporus sanguineus pyrosequencing RNA analysis RNA sequence sequence analysis xenobiotic metabolism Carbohydrate Metabolism Fungal Proteins Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Pycnoporus Transcriptome |
| spellingShingle |
beta tubulin carbohydrate esterase copper radical oxidase cytochrome p450 oxygenase elongation factor 1alpha enzyme epoxide hydrolase glutathione transferase glyceraldehyde 3 phosphate dehydrogenase glycosidase GMC oxidoreductase laccase lignin peroxidase lignocellulose manganese peroxidase peroxidase polysaccharide lyase RNA polymerase II sesquiterpenoid transcriptome triterpenoid unclassified drug article controlled study culture medium degradation enzyme modification enzyme synthesis homeostasis hydrolysis nonhuman nucleotide sequence protein database protein domain Pycnoporus Pycnoporus sanguineus pyrosequencing RNA analysis RNA sequence sequence analysis xenobiotic metabolism Carbohydrate Metabolism Fungal Proteins Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Pycnoporus Transcriptome A first insight into pycnoporus sanguineus BAFC 2126 transcriptome |
| topic_facet |
beta tubulin carbohydrate esterase copper radical oxidase cytochrome p450 oxygenase elongation factor 1alpha enzyme epoxide hydrolase glutathione transferase glyceraldehyde 3 phosphate dehydrogenase glycosidase GMC oxidoreductase laccase lignin peroxidase lignocellulose manganese peroxidase peroxidase polysaccharide lyase RNA polymerase II sesquiterpenoid transcriptome triterpenoid unclassified drug article controlled study culture medium degradation enzyme modification enzyme synthesis homeostasis hydrolysis nonhuman nucleotide sequence protein database protein domain Pycnoporus Pycnoporus sanguineus pyrosequencing RNA analysis RNA sequence sequence analysis xenobiotic metabolism Carbohydrate Metabolism Fungal Proteins Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Pycnoporus Transcriptome |
| description |
Fungi of the genus Pycnoporus are white-rot basidiomycetes widely studied because of their ability to synthesize high added-value compounds and enzymes of industrial interest. Here we report the sequencing, assembly and analysis of the transcriptome of Pycnoporus sanguineus BAFC 2126 grown at stationary phase, in media supplemented with copper sulfate. Using the 454 pyrosequencing platform we obtained a total of 226,336 reads (88,779,843 bases) that were filtered and de novo assembled to generate a reference transcriptome of 7,303 transcripts. Putative functions were assigned for 4,732 transcripts by searching similarities of six-frame translated sequences against a customized protein database and by the presence of conserved protein domains. Through the analysis of translated sequences we identified transcripts encoding 178 putative carbohydrate active enzymes, including representatives of 15 families with roles in lignocellulose degradation. Furthermore, we found many transcripts encoding enzymes related to lignin hydrolysis and modification, including laccases and peroxidases, as well as GMC oxidoreductases, copper radical oxidases and other enzymes involved in the generation of extracellular hydrogen peroxide and iron homeostasis. Finally, we identified the transcripts encoding all of the enzymes involved in terpenoid backbone biosynthesis pathway, various terpene synthases related to the biosynthesis of sesquiterpenoids and triterpenoids precursors, and also cytochrome P450 monooxygenases, glutathione S-transferases and epoxide hydrolases with potential functions in the biodegradation of xenobiotics and the enantioselective biosynthesis of biologically active drugs. To our knowledge this is the first report of a transcriptome of genus Pycnoporus and a resource for future molecular studies in P. sanguineus. Copyright: © 2013 Rohr et al. |
| title |
A first insight into pycnoporus sanguineus BAFC 2126 transcriptome |
| title_short |
A first insight into pycnoporus sanguineus BAFC 2126 transcriptome |
| title_full |
A first insight into pycnoporus sanguineus BAFC 2126 transcriptome |
| title_fullStr |
A first insight into pycnoporus sanguineus BAFC 2126 transcriptome |
| title_full_unstemmed |
A first insight into pycnoporus sanguineus BAFC 2126 transcriptome |
| title_sort |
first insight into pycnoporus sanguineus bafc 2126 transcriptome |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n12_p_Rohr http://hdl.handle.net/20.500.12110/paper_19326203_v8_n12_p_Rohr |
| _version_ |
1768545484522127360 |