The Global Anaerobic Regulator Anr, Is Involved in Cell Attachment and Aggregation Influencing the First Stages of Biofilm Development in Pseudomonas extremaustralis

Pseudomonas extremaustralis is a versatile Antarctic bacterium, able to grow under microaerobic and anaerobic conditions and is related to several non-pathogenic Pseudomonads. Here we report on the role of the global anaerobic regulator Anr, in the early steps of P. extremaustralis biofilm developme...

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Detalles Bibliográficos
Publicado: 2013
Materias:
Anr gene
article
bacterial flagellum
bacterial gene
bacterial genome
bacterial growth
bacterial phenomena and functions
binding site
biofilm
biomass
biomicroscopy
cell adhesion
cell aggregation
controlled study
gene identification
gene mutation
gene targeting
nonhuman
Pseudomonas
Pseudomonas extremaustralis
quantitative analysis
real time polymerase chain reaction
regulator gene
signal transduction
swimming motility
twitching motility
wild type
Aerobiosis
Anaerobiosis
Bacterial Adhesion
Bacterial Proteins
Base Sequence
Biofilms
DNA, Intergenic
Gene Expression Regulation, Bacterial
Genes, Bacterial
Molecular Sequence Data
Movement
Mutation
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n10_p_Tribelli
http://hdl.handle.net/20.500.12110/paper_19326203_v8_n10_p_Tribelli
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Pseudomonas extremaustralis is a versatile Antarctic bacterium, able to grow under microaerobic and anaerobic conditions and is related to several non-pathogenic Pseudomonads. Here we report on the role of the global anaerobic regulator Anr, in the early steps of P. extremaustralis biofilm development. We found that the anr mutant was reduced in its ability to attach, to form aggregates and to display twitching motility but presented higher swimming motility than the wild type. In addition, microscopy revealed that the wild type biofilm contained more biomass and was thicker, but were less rough than that of the anr mutant. In silico analysis of the P. extremaustralis genome for Anr-like binding sites led to the identification of two biofilm-related genes as potential targets of this regulator. When measured using Quantitative Real Time PCR, we found that the anr mutant expressed lower levels of pilG, which encodes a component of Type IV pili and has been previously implicated in cellular adhesion. Levels of morA, involved in signal transduction and flagella development, were also lower in the mutant. Our data suggest that under low oxygen conditions, such as those encountered in biofilms, Anr differentially regulates aggregation and motility thus affecting the first stages of biofilm formation. © 2013 Tribelli et al.

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