The Global Anaerobic Regulator Anr, Is Involved in Cell Attachment and Aggregation Influencing the First Stages of Biofilm Development in Pseudomonas extremaustralis
Pseudomonas extremaustralis is a versatile Antarctic bacterium, able to grow under microaerobic and anaerobic conditions and is related to several non-pathogenic Pseudomonads. Here we report on the role of the global anaerobic regulator Anr, in the early steps of P. extremaustralis biofilm developme...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n10_p_Tribelli http://hdl.handle.net/20.500.12110/paper_19326203_v8_n10_p_Tribelli |
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paper:paper_19326203_v8_n10_p_Tribelli2023-06-08T16:31:10Z The Global Anaerobic Regulator Anr, Is Involved in Cell Attachment and Aggregation Influencing the First Stages of Biofilm Development in Pseudomonas extremaustralis Anr gene article bacterial flagellum bacterial gene bacterial genome bacterial growth bacterial phenomena and functions binding site biofilm biomass biomicroscopy cell adhesion cell aggregation controlled study gene identification gene mutation gene targeting nonhuman Pseudomonas Pseudomonas extremaustralis quantitative analysis real time polymerase chain reaction regulator gene signal transduction swimming motility twitching motility wild type Aerobiosis Anaerobiosis Bacterial Adhesion Bacterial Proteins Base Sequence Biofilms DNA, Intergenic Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Movement Mutation Pseudomonas Pseudomonas extremaustralis is a versatile Antarctic bacterium, able to grow under microaerobic and anaerobic conditions and is related to several non-pathogenic Pseudomonads. Here we report on the role of the global anaerobic regulator Anr, in the early steps of P. extremaustralis biofilm development. We found that the anr mutant was reduced in its ability to attach, to form aggregates and to display twitching motility but presented higher swimming motility than the wild type. In addition, microscopy revealed that the wild type biofilm contained more biomass and was thicker, but were less rough than that of the anr mutant. In silico analysis of the P. extremaustralis genome for Anr-like binding sites led to the identification of two biofilm-related genes as potential targets of this regulator. When measured using Quantitative Real Time PCR, we found that the anr mutant expressed lower levels of pilG, which encodes a component of Type IV pili and has been previously implicated in cellular adhesion. Levels of morA, involved in signal transduction and flagella development, were also lower in the mutant. Our data suggest that under low oxygen conditions, such as those encountered in biofilms, Anr differentially regulates aggregation and motility thus affecting the first stages of biofilm formation. © 2013 Tribelli et al. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n10_p_Tribelli http://hdl.handle.net/20.500.12110/paper_19326203_v8_n10_p_Tribelli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Anr gene article bacterial flagellum bacterial gene bacterial genome bacterial growth bacterial phenomena and functions binding site biofilm biomass biomicroscopy cell adhesion cell aggregation controlled study gene identification gene mutation gene targeting nonhuman Pseudomonas Pseudomonas extremaustralis quantitative analysis real time polymerase chain reaction regulator gene signal transduction swimming motility twitching motility wild type Aerobiosis Anaerobiosis Bacterial Adhesion Bacterial Proteins Base Sequence Biofilms DNA, Intergenic Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Movement Mutation Pseudomonas |
| spellingShingle |
Anr gene article bacterial flagellum bacterial gene bacterial genome bacterial growth bacterial phenomena and functions binding site biofilm biomass biomicroscopy cell adhesion cell aggregation controlled study gene identification gene mutation gene targeting nonhuman Pseudomonas Pseudomonas extremaustralis quantitative analysis real time polymerase chain reaction regulator gene signal transduction swimming motility twitching motility wild type Aerobiosis Anaerobiosis Bacterial Adhesion Bacterial Proteins Base Sequence Biofilms DNA, Intergenic Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Movement Mutation Pseudomonas The Global Anaerobic Regulator Anr, Is Involved in Cell Attachment and Aggregation Influencing the First Stages of Biofilm Development in Pseudomonas extremaustralis |
| topic_facet |
Anr gene article bacterial flagellum bacterial gene bacterial genome bacterial growth bacterial phenomena and functions binding site biofilm biomass biomicroscopy cell adhesion cell aggregation controlled study gene identification gene mutation gene targeting nonhuman Pseudomonas Pseudomonas extremaustralis quantitative analysis real time polymerase chain reaction regulator gene signal transduction swimming motility twitching motility wild type Aerobiosis Anaerobiosis Bacterial Adhesion Bacterial Proteins Base Sequence Biofilms DNA, Intergenic Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Movement Mutation Pseudomonas |
| description |
Pseudomonas extremaustralis is a versatile Antarctic bacterium, able to grow under microaerobic and anaerobic conditions and is related to several non-pathogenic Pseudomonads. Here we report on the role of the global anaerobic regulator Anr, in the early steps of P. extremaustralis biofilm development. We found that the anr mutant was reduced in its ability to attach, to form aggregates and to display twitching motility but presented higher swimming motility than the wild type. In addition, microscopy revealed that the wild type biofilm contained more biomass and was thicker, but were less rough than that of the anr mutant. In silico analysis of the P. extremaustralis genome for Anr-like binding sites led to the identification of two biofilm-related genes as potential targets of this regulator. When measured using Quantitative Real Time PCR, we found that the anr mutant expressed lower levels of pilG, which encodes a component of Type IV pili and has been previously implicated in cellular adhesion. Levels of morA, involved in signal transduction and flagella development, were also lower in the mutant. Our data suggest that under low oxygen conditions, such as those encountered in biofilms, Anr differentially regulates aggregation and motility thus affecting the first stages of biofilm formation. © 2013 Tribelli et al. |
| title |
The Global Anaerobic Regulator Anr, Is Involved in Cell Attachment and Aggregation Influencing the First Stages of Biofilm Development in Pseudomonas extremaustralis |
| title_short |
The Global Anaerobic Regulator Anr, Is Involved in Cell Attachment and Aggregation Influencing the First Stages of Biofilm Development in Pseudomonas extremaustralis |
| title_full |
The Global Anaerobic Regulator Anr, Is Involved in Cell Attachment and Aggregation Influencing the First Stages of Biofilm Development in Pseudomonas extremaustralis |
| title_fullStr |
The Global Anaerobic Regulator Anr, Is Involved in Cell Attachment and Aggregation Influencing the First Stages of Biofilm Development in Pseudomonas extremaustralis |
| title_full_unstemmed |
The Global Anaerobic Regulator Anr, Is Involved in Cell Attachment and Aggregation Influencing the First Stages of Biofilm Development in Pseudomonas extremaustralis |
| title_sort |
global anaerobic regulator anr, is involved in cell attachment and aggregation influencing the first stages of biofilm development in pseudomonas extremaustralis |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n10_p_Tribelli http://hdl.handle.net/20.500.12110/paper_19326203_v8_n10_p_Tribelli |
| _version_ |
1768545393467981824 |