Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster.

Previously, we have shown there is clinal variation for egg-to-adult developmental time along geographic gradients in Drosophila melanogaster. Further, we also have identified mutations in genes involved in metabolic and neurogenic pathways that affect development time (heterochronic genes). However...

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Detalles Bibliográficos
Autores principales: Mensch, Julián, Carreira, Valeria Paula, Lavagnino, Nicolas Jose, Goenaga, Julieta, Folguera, Guillermo, Hasson, Esteban Ruben, Fanara, Juan Jose
Publicado: 2010
Materias:
animal
article
Drosophila melanogaster
genetic complementation
genetic variability
genetics
growth, development and aging
mutation
Animals
Genetic Complementation Test
Genetic Variation
Mutation
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v5_n6_p_Mensch
http://hdl.handle.net/20.500.12110/paper_19326203_v5_n6_p_Mensch
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_19326203_v5_n6_p_Mensch
record_format dspace
spelling paper:paper_19326203_v5_n6_p_Mensch2023-06-08T16:30:54Z Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster. Mensch, Julián Carreira, Valeria Paula Lavagnino, Nicolas Jose Goenaga, Julieta Folguera, Guillermo Hasson, Esteban Ruben Fanara, Juan Jose animal article Drosophila melanogaster genetic complementation genetic variability genetics growth, development and aging mutation Animals Drosophila melanogaster Genetic Complementation Test Genetic Variation Mutation Previously, we have shown there is clinal variation for egg-to-adult developmental time along geographic gradients in Drosophila melanogaster. Further, we also have identified mutations in genes involved in metabolic and neurogenic pathways that affect development time (heterochronic genes). However, we do not know whether these loci affect variation in developmental time in natural populations. Here, we constructed second chromosome substitution lines from natural populations of Drosophila melanogaster from an altitudinal cline, and measured egg-adult development time for each line. We found not only a large amount of genetic variation for developmental time, but also positive associations of the development time with thermal amplitude and altitude. We performed genetic complementation tests using substitution lines with the longest and shortest developmental times and heterochronic mutations. We identified segregating variation for neurogenic and metabolic genes that largely affected the duration of the larval stages but had no impact on the timing of metamorphosis. Altitudinal clinal variation in developmental time for natural chromosome substitution lines provides a unique opportunity to dissect the response of heterochronic genes to environmental gradients. Ontogenetic stage-specific variation in invected, mastermind, cricklet and CG14591 may affect natural variation in development time and thermal evolution. Fil:Mensch, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carreira, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lavagnino, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Goenaga, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Folguera, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fanara, J.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v5_n6_p_Mensch http://hdl.handle.net/20.500.12110/paper_19326203_v5_n6_p_Mensch
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic animal
article
Drosophila melanogaster
genetic complementation
genetic variability
genetics
growth, development and aging
mutation
Animals
Drosophila melanogaster
Genetic Complementation Test
Genetic Variation
Mutation
spellingShingle animal
article
Drosophila melanogaster
genetic complementation
genetic variability
genetics
growth, development and aging
mutation
Animals
Drosophila melanogaster
Genetic Complementation Test
Genetic Variation
Mutation
Mensch, Julián
Carreira, Valeria Paula
Lavagnino, Nicolas Jose
Goenaga, Julieta
Folguera, Guillermo
Hasson, Esteban Ruben
Fanara, Juan Jose
Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster.
topic_facet animal
article
Drosophila melanogaster
genetic complementation
genetic variability
genetics
growth, development and aging
mutation
Animals
Drosophila melanogaster
Genetic Complementation Test
Genetic Variation
Mutation
description Previously, we have shown there is clinal variation for egg-to-adult developmental time along geographic gradients in Drosophila melanogaster. Further, we also have identified mutations in genes involved in metabolic and neurogenic pathways that affect development time (heterochronic genes). However, we do not know whether these loci affect variation in developmental time in natural populations. Here, we constructed second chromosome substitution lines from natural populations of Drosophila melanogaster from an altitudinal cline, and measured egg-adult development time for each line. We found not only a large amount of genetic variation for developmental time, but also positive associations of the development time with thermal amplitude and altitude. We performed genetic complementation tests using substitution lines with the longest and shortest developmental times and heterochronic mutations. We identified segregating variation for neurogenic and metabolic genes that largely affected the duration of the larval stages but had no impact on the timing of metamorphosis. Altitudinal clinal variation in developmental time for natural chromosome substitution lines provides a unique opportunity to dissect the response of heterochronic genes to environmental gradients. Ontogenetic stage-specific variation in invected, mastermind, cricklet and CG14591 may affect natural variation in development time and thermal evolution.
author Mensch, Julián
Carreira, Valeria Paula
Lavagnino, Nicolas Jose
Goenaga, Julieta
Folguera, Guillermo
Hasson, Esteban Ruben
Fanara, Juan Jose
author_facet Mensch, Julián
Carreira, Valeria Paula
Lavagnino, Nicolas Jose
Goenaga, Julieta
Folguera, Guillermo
Hasson, Esteban Ruben
Fanara, Juan Jose
author_sort Mensch, Julián
title Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster.
title_short Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster.
title_full Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster.
title_fullStr Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster.
title_full_unstemmed Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster.
title_sort stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of drosophila melanogaster.
publishDate 2010
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v5_n6_p_Mensch
http://hdl.handle.net/20.500.12110/paper_19326203_v5_n6_p_Mensch
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