Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster
Background: Previously, we have shown there is clinal variation for egg-to-adult developmental time along geographic gradients in Drosophila melanogaster. Further, we also have identified mutations in genes involved in metabolic and neurogenic pathways that affect development time (heterochronic gen...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v5_n6_p1_Mensch http://hdl.handle.net/20.500.12110/paper_19326203_v5_n6_p1_Mensch |
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paper:paper_19326203_v5_n6_p1_Mensch2023-06-08T16:30:54Z Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster cricklet protein gene product invected protein mastermind protein protein CG14591 unclassified drug altitude acclimatization animal cell article chromosome substitution controlled study developmental stage Drosophila melanogaster female genetic complementation genetic variability larval stage metamorphosis nonhuman oocyte development thermal stimulation Background: Previously, we have shown there is clinal variation for egg-to-adult developmental time along geographic gradients in Drosophila melanogaster. Further, we also have identified mutations in genes involved in metabolic and neurogenic pathways that affect development time (heterochronic genes). However, we do not know whether these loci affect variation in developmental time in natural populations. Methodology/Principal Findings: Here, we constructed second chromosome substitution lines from natural populations of Drosophila melanogaster from an altitudinal cline, and measured egg-adult development time for each line. We found not only a large amount of genetic variation for developmental time, but also positive associations of the development time with thermal amplitude and altitude. We performed genetic complementation tests using substitution lines with the longest and shortest developmental times and heterochronic mutations. We identified segregating variation for neurogenic and metabolic genes that largely affected the duration of the larval stages but had no impact on the timing of metamorphosis. Conclusions/Significance: Altitudinal clinal variation in developmental time for natural chromosome substitution lines provides a unique opportunity to dissect the response of heterochronic genes to environmental gradients. Ontogenetic stage-specific variation in invected, mastermind, cricklet and CG14591 may affect natural variation in development time and thermal evolution. © 2010 Mensch et al. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v5_n6_p1_Mensch http://hdl.handle.net/20.500.12110/paper_19326203_v5_n6_p1_Mensch |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cricklet protein gene product invected protein mastermind protein protein CG14591 unclassified drug altitude acclimatization animal cell article chromosome substitution controlled study developmental stage Drosophila melanogaster female genetic complementation genetic variability larval stage metamorphosis nonhuman oocyte development thermal stimulation |
| spellingShingle |
cricklet protein gene product invected protein mastermind protein protein CG14591 unclassified drug altitude acclimatization animal cell article chromosome substitution controlled study developmental stage Drosophila melanogaster female genetic complementation genetic variability larval stage metamorphosis nonhuman oocyte development thermal stimulation Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster |
| topic_facet |
cricklet protein gene product invected protein mastermind protein protein CG14591 unclassified drug altitude acclimatization animal cell article chromosome substitution controlled study developmental stage Drosophila melanogaster female genetic complementation genetic variability larval stage metamorphosis nonhuman oocyte development thermal stimulation |
| description |
Background: Previously, we have shown there is clinal variation for egg-to-adult developmental time along geographic gradients in Drosophila melanogaster. Further, we also have identified mutations in genes involved in metabolic and neurogenic pathways that affect development time (heterochronic genes). However, we do not know whether these loci affect variation in developmental time in natural populations. Methodology/Principal Findings: Here, we constructed second chromosome substitution lines from natural populations of Drosophila melanogaster from an altitudinal cline, and measured egg-adult development time for each line. We found not only a large amount of genetic variation for developmental time, but also positive associations of the development time with thermal amplitude and altitude. We performed genetic complementation tests using substitution lines with the longest and shortest developmental times and heterochronic mutations. We identified segregating variation for neurogenic and metabolic genes that largely affected the duration of the larval stages but had no impact on the timing of metamorphosis. Conclusions/Significance: Altitudinal clinal variation in developmental time for natural chromosome substitution lines provides a unique opportunity to dissect the response of heterochronic genes to environmental gradients. Ontogenetic stage-specific variation in invected, mastermind, cricklet and CG14591 may affect natural variation in development time and thermal evolution. © 2010 Mensch et al. |
| title |
Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster |
| title_short |
Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster |
| title_full |
Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster |
| title_fullStr |
Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster |
| title_full_unstemmed |
Stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of Drosophila melanogaster |
| title_sort |
stage-specific effects of candidate heterochronic genes on variation in developmental time along an altitudinal cline of drosophila melanogaster |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v5_n6_p1_Mensch http://hdl.handle.net/20.500.12110/paper_19326203_v5_n6_p1_Mensch |
| _version_ |
1768544386110455808 |