Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
Electrogenic bacteria are organisms that can transfer electrons to extracellular electron acceptors and have the potential to be used in devices such as bioelectrochemical systems (BES). In this study, Dietzia sp. RNV-4 bacterium has been isolated and identified based on its biochemical, physiologic...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v12_n2_p_Sacco http://hdl.handle.net/20.500.12110/paper_19326203_v12_n2_p_Sacco |
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paper:paper_19326203_v12_n2_p_Sacco2023-06-08T16:30:38Z Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4 Sacco, Natalia Jimena Bonetto, Marìa Celina Cortón, Eduardo canthaxanthin RNA 16S canthaxanthin RNA 16S Article bacterial phenomena and functions bacterial strain bacterium isolation biochemistry bioelectrochemistry biofilm controlled study cyclic potentiometry density Dietzia Dietzia RNV 4 electrochemistry electrogenic bacterium electron transport high performance liquid chromatography microbial fuel cell new species nonhuman nucleotide sequence RNA sequence Actinobacteria bioenergy confocal microscopy electrochemical analysis electron genetics isolation and purification metabolism microbiology oxidation reduction reaction phylogeny procedures scanning electron microscopy Actinobacteria Bioelectric Energy Sources Biofilms Canthaxanthin Electrochemical Techniques Electron Transport Electrons Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S Electrogenic bacteria are organisms that can transfer electrons to extracellular electron acceptors and have the potential to be used in devices such as bioelectrochemical systems (BES). In this study, Dietzia sp. RNV-4 bacterium has been isolated and identified based on its biochemical, physiological and morphological characteristics, as well as by its 16S rRNA sequence analysis. Furthermore, the current density production and electron transfer mechanisms were investigated using bioelectrochemical methods. The chronoamperometric data showed that the biofilm of Dietzia sp. RNV-4 grew as the current increased with time, reaching a maximum of 176.6 ± 66.1 mA/m2 at the end of the experiment (7 d); this highly suggests that the current was generated by the biofilm. The main electron transfer mechanism, indicated by the cyclic voltammograms, was due to secreted redox mediators. By high performance liquid chromatography, canthaxanthin was identified as the main compound involved in charge transfer between the bacteria and the solid electrodes. Dietzia sp. RNV-4 was used as biological material in a microbial fuel cell (MFC) and the current density production was 299.4 ± 40.2 mA/m2. This is the first time that Dietzia sp. RNV-4 has been electrochemically characterized and identified as a new electrogenic strain. © 2017 Sacco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fil:Sacco, N.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bonetto, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cortón, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v12_n2_p_Sacco http://hdl.handle.net/20.500.12110/paper_19326203_v12_n2_p_Sacco |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
canthaxanthin RNA 16S canthaxanthin RNA 16S Article bacterial phenomena and functions bacterial strain bacterium isolation biochemistry bioelectrochemistry biofilm controlled study cyclic potentiometry density Dietzia Dietzia RNV 4 electrochemistry electrogenic bacterium electron transport high performance liquid chromatography microbial fuel cell new species nonhuman nucleotide sequence RNA sequence Actinobacteria bioenergy confocal microscopy electrochemical analysis electron genetics isolation and purification metabolism microbiology oxidation reduction reaction phylogeny procedures scanning electron microscopy Actinobacteria Bioelectric Energy Sources Biofilms Canthaxanthin Electrochemical Techniques Electron Transport Electrons Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S |
| spellingShingle |
canthaxanthin RNA 16S canthaxanthin RNA 16S Article bacterial phenomena and functions bacterial strain bacterium isolation biochemistry bioelectrochemistry biofilm controlled study cyclic potentiometry density Dietzia Dietzia RNV 4 electrochemistry electrogenic bacterium electron transport high performance liquid chromatography microbial fuel cell new species nonhuman nucleotide sequence RNA sequence Actinobacteria bioenergy confocal microscopy electrochemical analysis electron genetics isolation and purification metabolism microbiology oxidation reduction reaction phylogeny procedures scanning electron microscopy Actinobacteria Bioelectric Energy Sources Biofilms Canthaxanthin Electrochemical Techniques Electron Transport Electrons Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S Sacco, Natalia Jimena Bonetto, Marìa Celina Cortón, Eduardo Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4 |
| topic_facet |
canthaxanthin RNA 16S canthaxanthin RNA 16S Article bacterial phenomena and functions bacterial strain bacterium isolation biochemistry bioelectrochemistry biofilm controlled study cyclic potentiometry density Dietzia Dietzia RNV 4 electrochemistry electrogenic bacterium electron transport high performance liquid chromatography microbial fuel cell new species nonhuman nucleotide sequence RNA sequence Actinobacteria bioenergy confocal microscopy electrochemical analysis electron genetics isolation and purification metabolism microbiology oxidation reduction reaction phylogeny procedures scanning electron microscopy Actinobacteria Bioelectric Energy Sources Biofilms Canthaxanthin Electrochemical Techniques Electron Transport Electrons Microscopy, Confocal Microscopy, Electron, Scanning Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S |
| description |
Electrogenic bacteria are organisms that can transfer electrons to extracellular electron acceptors and have the potential to be used in devices such as bioelectrochemical systems (BES). In this study, Dietzia sp. RNV-4 bacterium has been isolated and identified based on its biochemical, physiological and morphological characteristics, as well as by its 16S rRNA sequence analysis. Furthermore, the current density production and electron transfer mechanisms were investigated using bioelectrochemical methods. The chronoamperometric data showed that the biofilm of Dietzia sp. RNV-4 grew as the current increased with time, reaching a maximum of 176.6 ± 66.1 mA/m2 at the end of the experiment (7 d); this highly suggests that the current was generated by the biofilm. The main electron transfer mechanism, indicated by the cyclic voltammograms, was due to secreted redox mediators. By high performance liquid chromatography, canthaxanthin was identified as the main compound involved in charge transfer between the bacteria and the solid electrodes. Dietzia sp. RNV-4 was used as biological material in a microbial fuel cell (MFC) and the current density production was 299.4 ± 40.2 mA/m2. This is the first time that Dietzia sp. RNV-4 has been electrochemically characterized and identified as a new electrogenic strain. © 2017 Sacco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| author |
Sacco, Natalia Jimena Bonetto, Marìa Celina Cortón, Eduardo |
| author_facet |
Sacco, Natalia Jimena Bonetto, Marìa Celina Cortón, Eduardo |
| author_sort |
Sacco, Natalia Jimena |
| title |
Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4 |
| title_short |
Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4 |
| title_full |
Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4 |
| title_fullStr |
Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4 |
| title_full_unstemmed |
Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4 |
| title_sort |
isolation and characterization of a novel electrogenic bacterium, dietzia sp. rnv-4 |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v12_n2_p_Sacco http://hdl.handle.net/20.500.12110/paper_19326203_v12_n2_p_Sacco |
| work_keys_str_mv |
AT sacconataliajimena isolationandcharacterizationofanovelelectrogenicbacteriumdietziasprnv4 AT bonettomariacelina isolationandcharacterizationofanovelelectrogenicbacteriumdietziasprnv4 AT cortoneduardo isolationandcharacterizationofanovelelectrogenicbacteriumdietziasprnv4 |
| _version_ |
1768542477528072192 |