Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster

Body size is a complex character associated to several fitness related traits that vary within and between species as a consequence of environmental and genetic factors. Latitudinal and altitudinal clines for different morphological traits have been described in several species of Drosophila and pre...

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Autores principales: Carreira, Valeria Paula, Mensch, Julián, Hasson, Esteban Ruben, Fanara, Juan Jose
Publicado: 2016
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n7_p_Carreira
http://hdl.handle.net/20.500.12110/paper_19326203_v11_n7_p_Carreira
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spelling paper:paper_19326203_v11_n7_p_Carreira2023-06-08T16:30:35Z Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster Carreira, Valeria Paula Mensch, Julián Hasson, Esteban Ruben Fanara, Juan Jose Argentina chromosome substitution strain Drosophila melanogaster gene frequency gene mutation genetic variation human human experiment morphological trait natural population phenotype quantitative trait anatomy and histology animal Drosophila ecosystem gene genetic polymorphism genetics quantitative trait locus Animals Argentina Drosophila Ecosystem Genes, Insect Phenotype Polymorphism, Genetic Quantitative Trait Loci Quantitative Trait, Heritable Body size is a complex character associated to several fitness related traits that vary within and between species as a consequence of environmental and genetic factors. Latitudinal and altitudinal clines for different morphological traits have been described in several species of Drosophila and previous work identified genomic regions associated with such variation in D. melanogaster. However, the genetic factors that orchestrate morphological variation have been barely studied. Here, our main objective was to investigate genetic variation for different morphological traits associated to the second chromosome in natural populations of D. melanogaster along latitudinal and altitudinal gradients in Argentina. Our results revealed weak clinal signals and a strong population effect on morphological variation. Moreover, most pairwise comparisons between populations were significant. Our study also showed important within-population genetic variation, which must be associated to the second chromosome, as the lines are otherwise genetically identical. Next, we examined the contribution of different candidate genes to natural variation for these traits. We performed quantitative complementation tests using a battery of lines bearing mutated alleles at candidate genes located in the second chromosome and six second chromosome substitution lines derived from natural populations which exhibited divergent phenotypes. Results of complementation tests revealed that natural variation at all candidate genes studied, invected, Fasciclin 3, toucan, Reticulon-like1, jing and CG14478, affects the studied characters, suggesting that they are Quantitative Trait Genes for morphological traits. Finally, the phenotypic patterns observed suggest that different alleles of each gene might contribute to natural variation for morphological traits. However, non-additive effects cannot be ruled out, as wild-derived strains differ at myriads of second chromosome loci that may interact epistatically with mutant alleles.Copyright: © 2016 Carreira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fil:Carreira, V.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mensch, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fanara, J.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n7_p_Carreira http://hdl.handle.net/20.500.12110/paper_19326203_v11_n7_p_Carreira
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Argentina
chromosome substitution strain
Drosophila melanogaster
gene frequency
gene mutation
genetic variation
human
human experiment
morphological trait
natural population
phenotype
quantitative trait
anatomy and histology
animal
Drosophila
ecosystem
gene
genetic polymorphism
genetics
quantitative trait locus
Animals
Argentina
Drosophila
Ecosystem
Genes, Insect
Phenotype
Polymorphism, Genetic
Quantitative Trait Loci
Quantitative Trait, Heritable
spellingShingle Argentina
chromosome substitution strain
Drosophila melanogaster
gene frequency
gene mutation
genetic variation
human
human experiment
morphological trait
natural population
phenotype
quantitative trait
anatomy and histology
animal
Drosophila
ecosystem
gene
genetic polymorphism
genetics
quantitative trait locus
Animals
Argentina
Drosophila
Ecosystem
Genes, Insect
Phenotype
Polymorphism, Genetic
Quantitative Trait Loci
Quantitative Trait, Heritable
Carreira, Valeria Paula
Mensch, Julián
Hasson, Esteban Ruben
Fanara, Juan Jose
Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster
topic_facet Argentina
chromosome substitution strain
Drosophila melanogaster
gene frequency
gene mutation
genetic variation
human
human experiment
morphological trait
natural population
phenotype
quantitative trait
anatomy and histology
animal
Drosophila
ecosystem
gene
genetic polymorphism
genetics
quantitative trait locus
Animals
Argentina
Drosophila
Ecosystem
Genes, Insect
Phenotype
Polymorphism, Genetic
Quantitative Trait Loci
Quantitative Trait, Heritable
description Body size is a complex character associated to several fitness related traits that vary within and between species as a consequence of environmental and genetic factors. Latitudinal and altitudinal clines for different morphological traits have been described in several species of Drosophila and previous work identified genomic regions associated with such variation in D. melanogaster. However, the genetic factors that orchestrate morphological variation have been barely studied. Here, our main objective was to investigate genetic variation for different morphological traits associated to the second chromosome in natural populations of D. melanogaster along latitudinal and altitudinal gradients in Argentina. Our results revealed weak clinal signals and a strong population effect on morphological variation. Moreover, most pairwise comparisons between populations were significant. Our study also showed important within-population genetic variation, which must be associated to the second chromosome, as the lines are otherwise genetically identical. Next, we examined the contribution of different candidate genes to natural variation for these traits. We performed quantitative complementation tests using a battery of lines bearing mutated alleles at candidate genes located in the second chromosome and six second chromosome substitution lines derived from natural populations which exhibited divergent phenotypes. Results of complementation tests revealed that natural variation at all candidate genes studied, invected, Fasciclin 3, toucan, Reticulon-like1, jing and CG14478, affects the studied characters, suggesting that they are Quantitative Trait Genes for morphological traits. Finally, the phenotypic patterns observed suggest that different alleles of each gene might contribute to natural variation for morphological traits. However, non-additive effects cannot be ruled out, as wild-derived strains differ at myriads of second chromosome loci that may interact epistatically with mutant alleles.Copyright: © 2016 Carreira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
author Carreira, Valeria Paula
Mensch, Julián
Hasson, Esteban Ruben
Fanara, Juan Jose
author_facet Carreira, Valeria Paula
Mensch, Julián
Hasson, Esteban Ruben
Fanara, Juan Jose
author_sort Carreira, Valeria Paula
title Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster
title_short Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster
title_full Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster
title_fullStr Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster
title_full_unstemmed Natural genetic variation and candidate genes for morphological traits in drosophila melanogaster
title_sort natural genetic variation and candidate genes for morphological traits in drosophila melanogaster
publishDate 2016
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n7_p_Carreira
http://hdl.handle.net/20.500.12110/paper_19326203_v11_n7_p_Carreira
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AT menschjulian naturalgeneticvariationandcandidategenesformorphologicaltraitsindrosophilamelanogaster
AT hassonestebanruben naturalgeneticvariationandcandidategenesformorphologicaltraitsindrosophilamelanogaster
AT fanarajuanjose naturalgeneticvariationandcandidategenesformorphologicaltraitsindrosophilamelanogaster
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