Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions
Scorpions of the genus Tityus show holokinetic chromosomes, achiasmatic male meiosis and an absence of heteromorphic sex chromosomes, like all Buthidae. In this work, we analysed the meiotic behaviour and chromosome rearrangements of a population of the scorpion Tityus confluens, characterising the...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n10_p_Adilardi http://hdl.handle.net/20.500.12110/paper_19326203_v11_n10_p_Adilardi |
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paper:paper_19326203_v11_n10_p_Adilardi2023-06-08T16:30:32Z Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions Adilardi, Renzo Sebastian Ojanguren Affilastro, Andrés Alejandro Mola, Liliana Maria animal cell animal tissue Article autosome cell fusion chromosome analysis chromosome number chromosome rearrangement controlled study cytotype embryo female fluorescence in situ hybridization heterozygosity karyotyping male meiosis mitosis nonhuman scorpion sex chromosome sex linkage Tityus confluens X chromosome Y chromosome animal chromosome segregation genetics growth, development and aging heterozygote metabolism nonmammalian embryo scorpion Animals Chromosome Segregation Embryo, Nonmammalian Female Heterozygote In Situ Hybridization, Fluorescence Karyotyping Male Meiosis Mitosis Scorpions Sex Chromosomes Scorpions of the genus Tityus show holokinetic chromosomes, achiasmatic male meiosis and an absence of heteromorphic sex chromosomes, like all Buthidae. In this work, we analysed the meiotic behaviour and chromosome rearrangements of a population of the scorpion Tityus confluens, characterising the cytotypes of males, females and embryos with different cytogenetic techniques. This revealed that all the females were structural homozygotes, while all the males were structural heterozygotes for different chromosome rearrangements. Four different cytotypes were described in males, which differed in chromosome number (2n = 5 and 2n = 6) and meiotic multivalent configurations (chains of four, five and six chromosomes). Based on a detailed mitotic and meiotic analysis, we propose a sequence of chromosome rearrangements that could give rise to each cytotype and in which fusions have played a major role. Based on the comparison of males, females and a brood of embryos, we also propose that the presence of multivalents in males and homologous pairs in females could be associated with the presence of cryptic sex chromosomes, with the male being the heterogametic sex. We propose that the ancestral karyotype of this species could have had homomorphic XY/XX (male/female) sex chromosomes and a fusion could have occurred between the Y chromosome and an autosome. © 2016 Adilardi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fil:Adilardi, R.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ojanguren-Affilastro, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mola, L.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n10_p_Adilardi http://hdl.handle.net/20.500.12110/paper_19326203_v11_n10_p_Adilardi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
animal cell animal tissue Article autosome cell fusion chromosome analysis chromosome number chromosome rearrangement controlled study cytotype embryo female fluorescence in situ hybridization heterozygosity karyotyping male meiosis mitosis nonhuman scorpion sex chromosome sex linkage Tityus confluens X chromosome Y chromosome animal chromosome segregation genetics growth, development and aging heterozygote metabolism nonmammalian embryo scorpion Animals Chromosome Segregation Embryo, Nonmammalian Female Heterozygote In Situ Hybridization, Fluorescence Karyotyping Male Meiosis Mitosis Scorpions Sex Chromosomes |
| spellingShingle |
animal cell animal tissue Article autosome cell fusion chromosome analysis chromosome number chromosome rearrangement controlled study cytotype embryo female fluorescence in situ hybridization heterozygosity karyotyping male meiosis mitosis nonhuman scorpion sex chromosome sex linkage Tityus confluens X chromosome Y chromosome animal chromosome segregation genetics growth, development and aging heterozygote metabolism nonmammalian embryo scorpion Animals Chromosome Segregation Embryo, Nonmammalian Female Heterozygote In Situ Hybridization, Fluorescence Karyotyping Male Meiosis Mitosis Scorpions Sex Chromosomes Adilardi, Renzo Sebastian Ojanguren Affilastro, Andrés Alejandro Mola, Liliana Maria Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions |
| topic_facet |
animal cell animal tissue Article autosome cell fusion chromosome analysis chromosome number chromosome rearrangement controlled study cytotype embryo female fluorescence in situ hybridization heterozygosity karyotyping male meiosis mitosis nonhuman scorpion sex chromosome sex linkage Tityus confluens X chromosome Y chromosome animal chromosome segregation genetics growth, development and aging heterozygote metabolism nonmammalian embryo scorpion Animals Chromosome Segregation Embryo, Nonmammalian Female Heterozygote In Situ Hybridization, Fluorescence Karyotyping Male Meiosis Mitosis Scorpions Sex Chromosomes |
| description |
Scorpions of the genus Tityus show holokinetic chromosomes, achiasmatic male meiosis and an absence of heteromorphic sex chromosomes, like all Buthidae. In this work, we analysed the meiotic behaviour and chromosome rearrangements of a population of the scorpion Tityus confluens, characterising the cytotypes of males, females and embryos with different cytogenetic techniques. This revealed that all the females were structural homozygotes, while all the males were structural heterozygotes for different chromosome rearrangements. Four different cytotypes were described in males, which differed in chromosome number (2n = 5 and 2n = 6) and meiotic multivalent configurations (chains of four, five and six chromosomes). Based on a detailed mitotic and meiotic analysis, we propose a sequence of chromosome rearrangements that could give rise to each cytotype and in which fusions have played a major role. Based on the comparison of males, females and a brood of embryos, we also propose that the presence of multivalents in males and homologous pairs in females could be associated with the presence of cryptic sex chromosomes, with the male being the heterogametic sex. We propose that the ancestral karyotype of this species could have had homomorphic XY/XX (male/female) sex chromosomes and a fusion could have occurred between the Y chromosome and an autosome. © 2016 Adilardi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| author |
Adilardi, Renzo Sebastian Ojanguren Affilastro, Andrés Alejandro Mola, Liliana Maria |
| author_facet |
Adilardi, Renzo Sebastian Ojanguren Affilastro, Andrés Alejandro Mola, Liliana Maria |
| author_sort |
Adilardi, Renzo Sebastian |
| title |
Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions |
| title_short |
Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions |
| title_full |
Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions |
| title_fullStr |
Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions |
| title_full_unstemmed |
Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions |
| title_sort |
sex-linked chromosome heterozygosity in males of tityus confluens (buthidae): a clue about the presence of sex chromosomes in scorpions |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n10_p_Adilardi http://hdl.handle.net/20.500.12110/paper_19326203_v11_n10_p_Adilardi |
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| _version_ |
1768543629632077824 |