Genetic and morphometric variability in caiman latirostris (broad-snouted caiman), reptilia, alligatoridae

Caiman latirostris (broad-snouted caiman) is a crocodilian species from Argentina subject of management plans. The goal of this study was estimating the distribution of genetic variability using RAPD markers and quantitative traits in wild populations of C. latirostris from Santa Fe province, Argent...

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Autores principales:	Amavet, Patricia Susana, Vilardi, Juan Cesar, Saidman, Beatriz Ofelia
Publicado:	2009
Materias:	primer DNA alligator analysis of variance animal Argentina article genetic marker genetic variability genetics histology morphometrics random amplified polymorphic DNA Alligators and Crocodiles Analysis of Variance Animals Body Weights and Measures DNA Primers Genetic Markers Genetic Variation Random Amplified Polymorphic DNA Technique Alligatorinae Animalia Caiman Caiman latirostris Crocodylidae (all crocodiles) Reptilia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19325223_v311_n4_p258_Amavet http://hdl.handle.net/20.500.12110/paper_19325223_v311_n4_p258_Amavet
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_19325223_v311_n4_p258_Amavet
record_format	dspace
spelling	paper:paper_19325223_v311_n4_p258_Amavet2023-06-08T16:30:23Z Genetic and morphometric variability in caiman latirostris (broad-snouted caiman), reptilia, alligatoridae Amavet, Patricia Susana Vilardi, Juan Cesar Saidman, Beatriz Ofelia primer DNA alligator analysis of variance animal Argentina article genetic marker genetic variability genetics histology morphometrics random amplified polymorphic DNA Alligators and Crocodiles Analysis of Variance Animals Argentina Body Weights and Measures DNA Primers Genetic Markers Genetic Variation Random Amplified Polymorphic DNA Technique Alligatorinae Animalia Caiman Caiman latirostris Crocodylidae (all crocodiles) Reptilia Caiman latirostris (broad-snouted caiman) is a crocodilian species from Argentina subject of management plans. The goal of this study was estimating the distribution of genetic variability using RAPD markers and quantitative traits in wild populations of C. latirostris from Santa Fe province, Argentina. We sampled animals from four populations to obtain DNA and morphometric measurements. Eight RAPD primers were used and PCR products were analyzed on 4% polyacrylamide gels stained with silver nitrate. Eleven allometric measurements were obtained in animals within 48 hr after birth. We were able to reveal a relatively high number of variable markers in the studied populations. Our estimates of polymorphism and heterozygosity are higher than recorded values in other crocodilians using isozymes, the studied populations showed low levels of gene flow and some population subdivision. The study of quantitative traits conducted by nested analysis of variance and principal component analysis indicated higher levels of variance among nests within populations than among populations. We found that some head measurements have the highest contribution to morphological differences among populations; this fact could support the role of these traits in reproductive or feeding behavior. Estimated genetic differentiation value (FST) among populations was higher than quantitative trait differentiation value (QST), suggesting a higher contribution of neutral than adaptive loci to the genetic differentiation among populations. Quantitative traits are probably more related with fitness and the differentiation among populations remained relatively lower. The high heritability estimated for some traits indicates great potential to improve them in management plans. J. Exp. Zool. 311A.258-269, 2009. © 2009 WILEY-LISS, INC. Fil:Amavet, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vilardi, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Saidman, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19325223_v311_n4_p258_Amavet http://hdl.handle.net/20.500.12110/paper_19325223_v311_n4_p258_Amavet
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	primer DNA alligator analysis of variance animal Argentina article genetic marker genetic variability genetics histology morphometrics random amplified polymorphic DNA Alligators and Crocodiles Analysis of Variance Animals Argentina Body Weights and Measures DNA Primers Genetic Markers Genetic Variation Random Amplified Polymorphic DNA Technique Alligatorinae Animalia Caiman Caiman latirostris Crocodylidae (all crocodiles) Reptilia
spellingShingle	primer DNA alligator analysis of variance animal Argentina article genetic marker genetic variability genetics histology morphometrics random amplified polymorphic DNA Alligators and Crocodiles Analysis of Variance Animals Argentina Body Weights and Measures DNA Primers Genetic Markers Genetic Variation Random Amplified Polymorphic DNA Technique Alligatorinae Animalia Caiman Caiman latirostris Crocodylidae (all crocodiles) Reptilia Amavet, Patricia Susana Vilardi, Juan Cesar Saidman, Beatriz Ofelia Genetic and morphometric variability in caiman latirostris (broad-snouted caiman), reptilia, alligatoridae
topic_facet	primer DNA alligator analysis of variance animal Argentina article genetic marker genetic variability genetics histology morphometrics random amplified polymorphic DNA Alligators and Crocodiles Analysis of Variance Animals Argentina Body Weights and Measures DNA Primers Genetic Markers Genetic Variation Random Amplified Polymorphic DNA Technique Alligatorinae Animalia Caiman Caiman latirostris Crocodylidae (all crocodiles) Reptilia
description	Caiman latirostris (broad-snouted caiman) is a crocodilian species from Argentina subject of management plans. The goal of this study was estimating the distribution of genetic variability using RAPD markers and quantitative traits in wild populations of C. latirostris from Santa Fe province, Argentina. We sampled animals from four populations to obtain DNA and morphometric measurements. Eight RAPD primers were used and PCR products were analyzed on 4% polyacrylamide gels stained with silver nitrate. Eleven allometric measurements were obtained in animals within 48 hr after birth. We were able to reveal a relatively high number of variable markers in the studied populations. Our estimates of polymorphism and heterozygosity are higher than recorded values in other crocodilians using isozymes, the studied populations showed low levels of gene flow and some population subdivision. The study of quantitative traits conducted by nested analysis of variance and principal component analysis indicated higher levels of variance among nests within populations than among populations. We found that some head measurements have the highest contribution to morphological differences among populations; this fact could support the role of these traits in reproductive or feeding behavior. Estimated genetic differentiation value (FST) among populations was higher than quantitative trait differentiation value (QST), suggesting a higher contribution of neutral than adaptive loci to the genetic differentiation among populations. Quantitative traits are probably more related with fitness and the differentiation among populations remained relatively lower. The high heritability estimated for some traits indicates great potential to improve them in management plans. J. Exp. Zool. 311A.258-269, 2009. © 2009 WILEY-LISS, INC.
author	Amavet, Patricia Susana Vilardi, Juan Cesar Saidman, Beatriz Ofelia
author_facet	Amavet, Patricia Susana Vilardi, Juan Cesar Saidman, Beatriz Ofelia
author_sort	Amavet, Patricia Susana
title	Genetic and morphometric variability in caiman latirostris (broad-snouted caiman), reptilia, alligatoridae
title_short	Genetic and morphometric variability in caiman latirostris (broad-snouted caiman), reptilia, alligatoridae
title_full	Genetic and morphometric variability in caiman latirostris (broad-snouted caiman), reptilia, alligatoridae
title_fullStr	Genetic and morphometric variability in caiman latirostris (broad-snouted caiman), reptilia, alligatoridae
title_full_unstemmed	Genetic and morphometric variability in caiman latirostris (broad-snouted caiman), reptilia, alligatoridae
title_sort	genetic and morphometric variability in caiman latirostris (broad-snouted caiman), reptilia, alligatoridae
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19325223_v311_n4_p258_Amavet http://hdl.handle.net/20.500.12110/paper_19325223_v311_n4_p258_Amavet
work_keys_str_mv	AT amavetpatriciasusana geneticandmorphometricvariabilityincaimanlatirostrisbroadsnoutedcaimanreptiliaalligatoridae AT vilardijuancesar geneticandmorphometricvariabilityincaimanlatirostrisbroadsnoutedcaimanreptiliaalligatoridae AT saidmanbeatrizofelia geneticandmorphometricvariabilityincaimanlatirostrisbroadsnoutedcaimanreptiliaalligatoridae
_version_	1768545622021898240

Genetic and morphometric variability in caiman latirostris (broad-snouted caiman), reptilia, alligatoridae

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