Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period
The use of lactic acid bacteria (LAB) in animal feed, constitute an alternative tool for bovine mastitis prevention. Previously, two LAB strains were isolated from bovine milk and selected for their probiotics properties. So far, immune response of inoculating LAB in bovine udders at dry-off period...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18762883_v8_n4_p589_Pellegrino http://hdl.handle.net/20.500.12110/paper_18762883_v8_n4_p589_Pellegrino |
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paper:paper_18762883_v8_n4_p589_Pellegrino2023-06-08T16:30:14Z Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period Bovine mastitis Lactic acid bacteria Lactobacillus Probiotics Staphylococcus bacterial antigen epitope immunoglobulin G antibody immunoglobulin G1 antibody immunoglobulin G2 antibody probiotic agent unclassified drug probiotic agent animal cell antibody blood level antibody detection antibody specificity Article bacterium detection blood analysis bovine mastitis cell count cell proliferation cellular immunity controlled study dairy cattle dry off period female humoral immunity immunomodulation in vitro study infection prevention inoculation intramammary inoculation lactic acid bacterium Lactobacillus delbrueckii subsp. lactis Lactobacillus delbrueckii subsp. lactis CRL1655 lymphocyte proliferation memory cell nonhuman somatic cell Staphylococcus aureus time time series analysis animal bovine bovine mastitis immunology Lactobacillus microbiology physiology Staphylococcus infection Animals Cattle Female Immunity, Cellular Immunity, Humoral Lactobacillus Mastitis, Bovine Probiotics Staphylococcal Infections Staphylococcus aureus The use of lactic acid bacteria (LAB) in animal feed, constitute an alternative tool for bovine mastitis prevention. Previously, two LAB strains were isolated from bovine milk and selected for their probiotics properties. So far, immune response of inoculating LAB in bovine udders at dry-off period has not been investigated. The immunoglobulin isotype levels and memory cell proliferation in blood and milk of animals inoculated with Lactobacillus lactis subsp. lactis CRL1655 and Lactobacillus perolens CRL1724 at dry-off period was studied. Ten animals were inoculated intramammarily with 106 cells of each LAB (IG) and 2 animals used as control (NIG). Milk and blood samples were taken before inoculation and 1, 2, 4, 6, 12 and 24 h and 7 and 14 days after inoculation. Somatic cell count (SCC) in milk, the presence of bovine mastitis pathogens, the levels of antibodies and lymphocyte proliferation were determined. In the IG, the SCC was < 250,000 cells/ml up to 4 h after intramammary inoculation. Six and 12 h after inoculation, the SCC increased up to 600,000 and 2,000,000 cells/ml, respectively. In the NIG, the SCC reached the maximum value 7 days after inoculation. Microbiological analysis showed that all samples were negative for major bovine mastitis pathogens after 24-48 h of incubation. In general, LAB inoculation increased the amount of IgG isotypes in blood and milk, and these antibodies were able to recognise Staphylococcus aureus epitopes. Lymphocytes proliferation was significantly higher in the IG at all time points assayed, following LAB or S. aureus stimulation. The lymphocytes of animals inoculated with LAB do not react in vitro to the presence of S. aureus antigen.. The results showed that probiotic microorganisms could be a natural and effective alternative in the prevention of bovine mastitis at dry-off period and act as immunomodulatory stimulating local and systemic defence lines. © 2017 Wageningen Academic Publishers. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18762883_v8_n4_p589_Pellegrino http://hdl.handle.net/20.500.12110/paper_18762883_v8_n4_p589_Pellegrino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bovine mastitis Lactic acid bacteria Lactobacillus Probiotics Staphylococcus bacterial antigen epitope immunoglobulin G antibody immunoglobulin G1 antibody immunoglobulin G2 antibody probiotic agent unclassified drug probiotic agent animal cell antibody blood level antibody detection antibody specificity Article bacterium detection blood analysis bovine mastitis cell count cell proliferation cellular immunity controlled study dairy cattle dry off period female humoral immunity immunomodulation in vitro study infection prevention inoculation intramammary inoculation lactic acid bacterium Lactobacillus delbrueckii subsp. lactis Lactobacillus delbrueckii subsp. lactis CRL1655 lymphocyte proliferation memory cell nonhuman somatic cell Staphylococcus aureus time time series analysis animal bovine bovine mastitis immunology Lactobacillus microbiology physiology Staphylococcus infection Animals Cattle Female Immunity, Cellular Immunity, Humoral Lactobacillus Mastitis, Bovine Probiotics Staphylococcal Infections Staphylococcus aureus |
| spellingShingle |
Bovine mastitis Lactic acid bacteria Lactobacillus Probiotics Staphylococcus bacterial antigen epitope immunoglobulin G antibody immunoglobulin G1 antibody immunoglobulin G2 antibody probiotic agent unclassified drug probiotic agent animal cell antibody blood level antibody detection antibody specificity Article bacterium detection blood analysis bovine mastitis cell count cell proliferation cellular immunity controlled study dairy cattle dry off period female humoral immunity immunomodulation in vitro study infection prevention inoculation intramammary inoculation lactic acid bacterium Lactobacillus delbrueckii subsp. lactis Lactobacillus delbrueckii subsp. lactis CRL1655 lymphocyte proliferation memory cell nonhuman somatic cell Staphylococcus aureus time time series analysis animal bovine bovine mastitis immunology Lactobacillus microbiology physiology Staphylococcus infection Animals Cattle Female Immunity, Cellular Immunity, Humoral Lactobacillus Mastitis, Bovine Probiotics Staphylococcal Infections Staphylococcus aureus Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period |
| topic_facet |
Bovine mastitis Lactic acid bacteria Lactobacillus Probiotics Staphylococcus bacterial antigen epitope immunoglobulin G antibody immunoglobulin G1 antibody immunoglobulin G2 antibody probiotic agent unclassified drug probiotic agent animal cell antibody blood level antibody detection antibody specificity Article bacterium detection blood analysis bovine mastitis cell count cell proliferation cellular immunity controlled study dairy cattle dry off period female humoral immunity immunomodulation in vitro study infection prevention inoculation intramammary inoculation lactic acid bacterium Lactobacillus delbrueckii subsp. lactis Lactobacillus delbrueckii subsp. lactis CRL1655 lymphocyte proliferation memory cell nonhuman somatic cell Staphylococcus aureus time time series analysis animal bovine bovine mastitis immunology Lactobacillus microbiology physiology Staphylococcus infection Animals Cattle Female Immunity, Cellular Immunity, Humoral Lactobacillus Mastitis, Bovine Probiotics Staphylococcal Infections Staphylococcus aureus |
| description |
The use of lactic acid bacteria (LAB) in animal feed, constitute an alternative tool for bovine mastitis prevention. Previously, two LAB strains were isolated from bovine milk and selected for their probiotics properties. So far, immune response of inoculating LAB in bovine udders at dry-off period has not been investigated. The immunoglobulin isotype levels and memory cell proliferation in blood and milk of animals inoculated with Lactobacillus lactis subsp. lactis CRL1655 and Lactobacillus perolens CRL1724 at dry-off period was studied. Ten animals were inoculated intramammarily with 106 cells of each LAB (IG) and 2 animals used as control (NIG). Milk and blood samples were taken before inoculation and 1, 2, 4, 6, 12 and 24 h and 7 and 14 days after inoculation. Somatic cell count (SCC) in milk, the presence of bovine mastitis pathogens, the levels of antibodies and lymphocyte proliferation were determined. In the IG, the SCC was < 250,000 cells/ml up to 4 h after intramammary inoculation. Six and 12 h after inoculation, the SCC increased up to 600,000 and 2,000,000 cells/ml, respectively. In the NIG, the SCC reached the maximum value 7 days after inoculation. Microbiological analysis showed that all samples were negative for major bovine mastitis pathogens after 24-48 h of incubation. In general, LAB inoculation increased the amount of IgG isotypes in blood and milk, and these antibodies were able to recognise Staphylococcus aureus epitopes. Lymphocytes proliferation was significantly higher in the IG at all time points assayed, following LAB or S. aureus stimulation. The lymphocytes of animals inoculated with LAB do not react in vitro to the presence of S. aureus antigen.. The results showed that probiotic microorganisms could be a natural and effective alternative in the prevention of bovine mastitis at dry-off period and act as immunomodulatory stimulating local and systemic defence lines. © 2017 Wageningen Academic Publishers. |
| title |
Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period |
| title_short |
Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period |
| title_full |
Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period |
| title_fullStr |
Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period |
| title_full_unstemmed |
Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period |
| title_sort |
bovine mastitis prevention: humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18762883_v8_n4_p589_Pellegrino http://hdl.handle.net/20.500.12110/paper_18762883_v8_n4_p589_Pellegrino |
| _version_ |
1768543057206050816 |