Transcriptional regulation of the protein kinase A subunits in Saccharomyces cerevisiae: Autoregulatory role of the kinase A activity
Protein kinase A (PKA) is a broad specificity protein kinase that controls a physiological response following the increment of cAMP as a consequence of a particular stimulus. The specificity of cAMP-signal transduction is maintained by several levels of control acting all together. Herein we present...
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paper:paper_18749399_v1839_n4_p275_Pautasso2023-06-08T16:30:08Z Transcriptional regulation of the protein kinase A subunits in Saccharomyces cerevisiae: Autoregulatory role of the kinase A activity Pautasso, Constanza Rossi, Silvia Graciela Bcy1 PKA Tpks Transcription regulation BCY1 protein cyclic AMP dependent protein kinase protein protein kinase protein kinase Rim15 protein kinase Yak1 TPK1 protein TPK2 protein TPK3 protein transcription factor transcription factor Gis1 transcription factor Msn2 transcription factor Msn4 transcription factor Sok2 unclassified drug article autoregulation controlled study enzyme activity growth curve heat shock nonhuman priority journal protein expression Saccharomyces cerevisiae transcription regulation Bcy1 PKA Tpks Transcription regulation Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Cyclic AMP-Dependent Protein Kinases Gene Expression Regulation, Fungal Hot Temperature Phosphorylation Promoter Regions, Genetic Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Stress, Physiological Transcription Factors Transcription, Genetic Protein kinase A (PKA) is a broad specificity protein kinase that controls a physiological response following the increment of cAMP as a consequence of a particular stimulus. The specificity of cAMP-signal transduction is maintained by several levels of control acting all together. Herein we present the study of the regulation of the expression of each PKA subunit, analyzing the activity of their promoters. The promoter of each isoform of TPK and of BCY1 is differentially activated during the growth phase. A negative mechanism of isoform-dependent autoregulation directs TPKs and BCY1 gene expressions. TPK1 promoter activity is positively regulated during heat shock and saline stress. The kinase Rim15, but not the kinase Yak1, positively regulates TPK1 promoter. Msn2/4, Gis1, and Sok2 are transcription factors involved in the regulation of TPK1 expression during stress. TPK2, TPK3, and BCY1 promoters, unlike TPK1, are not activated under stress conditions, although all the promoters are activated under low or null protein kinase A activity. These results indicate that subunits share an inhibitory autoregulatory mechanism but have different mechanisms involved in response to heat shock or saline stress. © 2014 Elsevier B.V. Fil:Pautasso, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossi, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18749399_v1839_n4_p275_Pautasso http://hdl.handle.net/20.500.12110/paper_18749399_v1839_n4_p275_Pautasso |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bcy1 PKA Tpks Transcription regulation BCY1 protein cyclic AMP dependent protein kinase protein protein kinase protein kinase Rim15 protein kinase Yak1 TPK1 protein TPK2 protein TPK3 protein transcription factor transcription factor Gis1 transcription factor Msn2 transcription factor Msn4 transcription factor Sok2 unclassified drug article autoregulation controlled study enzyme activity growth curve heat shock nonhuman priority journal protein expression Saccharomyces cerevisiae transcription regulation Bcy1 PKA Tpks Transcription regulation Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Cyclic AMP-Dependent Protein Kinases Gene Expression Regulation, Fungal Hot Temperature Phosphorylation Promoter Regions, Genetic Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Stress, Physiological Transcription Factors Transcription, Genetic |
| spellingShingle |
Bcy1 PKA Tpks Transcription regulation BCY1 protein cyclic AMP dependent protein kinase protein protein kinase protein kinase Rim15 protein kinase Yak1 TPK1 protein TPK2 protein TPK3 protein transcription factor transcription factor Gis1 transcription factor Msn2 transcription factor Msn4 transcription factor Sok2 unclassified drug article autoregulation controlled study enzyme activity growth curve heat shock nonhuman priority journal protein expression Saccharomyces cerevisiae transcription regulation Bcy1 PKA Tpks Transcription regulation Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Cyclic AMP-Dependent Protein Kinases Gene Expression Regulation, Fungal Hot Temperature Phosphorylation Promoter Regions, Genetic Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Stress, Physiological Transcription Factors Transcription, Genetic Pautasso, Constanza Rossi, Silvia Graciela Transcriptional regulation of the protein kinase A subunits in Saccharomyces cerevisiae: Autoregulatory role of the kinase A activity |
| topic_facet |
Bcy1 PKA Tpks Transcription regulation BCY1 protein cyclic AMP dependent protein kinase protein protein kinase protein kinase Rim15 protein kinase Yak1 TPK1 protein TPK2 protein TPK3 protein transcription factor transcription factor Gis1 transcription factor Msn2 transcription factor Msn4 transcription factor Sok2 unclassified drug article autoregulation controlled study enzyme activity growth curve heat shock nonhuman priority journal protein expression Saccharomyces cerevisiae transcription regulation Bcy1 PKA Tpks Transcription regulation Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Cyclic AMP-Dependent Protein Kinases Gene Expression Regulation, Fungal Hot Temperature Phosphorylation Promoter Regions, Genetic Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Stress, Physiological Transcription Factors Transcription, Genetic |
| description |
Protein kinase A (PKA) is a broad specificity protein kinase that controls a physiological response following the increment of cAMP as a consequence of a particular stimulus. The specificity of cAMP-signal transduction is maintained by several levels of control acting all together. Herein we present the study of the regulation of the expression of each PKA subunit, analyzing the activity of their promoters. The promoter of each isoform of TPK and of BCY1 is differentially activated during the growth phase. A negative mechanism of isoform-dependent autoregulation directs TPKs and BCY1 gene expressions. TPK1 promoter activity is positively regulated during heat shock and saline stress. The kinase Rim15, but not the kinase Yak1, positively regulates TPK1 promoter. Msn2/4, Gis1, and Sok2 are transcription factors involved in the regulation of TPK1 expression during stress. TPK2, TPK3, and BCY1 promoters, unlike TPK1, are not activated under stress conditions, although all the promoters are activated under low or null protein kinase A activity. These results indicate that subunits share an inhibitory autoregulatory mechanism but have different mechanisms involved in response to heat shock or saline stress. © 2014 Elsevier B.V. |
| author |
Pautasso, Constanza Rossi, Silvia Graciela |
| author_facet |
Pautasso, Constanza Rossi, Silvia Graciela |
| author_sort |
Pautasso, Constanza |
| title |
Transcriptional regulation of the protein kinase A subunits in Saccharomyces cerevisiae: Autoregulatory role of the kinase A activity |
| title_short |
Transcriptional regulation of the protein kinase A subunits in Saccharomyces cerevisiae: Autoregulatory role of the kinase A activity |
| title_full |
Transcriptional regulation of the protein kinase A subunits in Saccharomyces cerevisiae: Autoregulatory role of the kinase A activity |
| title_fullStr |
Transcriptional regulation of the protein kinase A subunits in Saccharomyces cerevisiae: Autoregulatory role of the kinase A activity |
| title_full_unstemmed |
Transcriptional regulation of the protein kinase A subunits in Saccharomyces cerevisiae: Autoregulatory role of the kinase A activity |
| title_sort |
transcriptional regulation of the protein kinase a subunits in saccharomyces cerevisiae: autoregulatory role of the kinase a activity |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18749399_v1839_n4_p275_Pautasso http://hdl.handle.net/20.500.12110/paper_18749399_v1839_n4_p275_Pautasso |
| work_keys_str_mv |
AT pautassoconstanza transcriptionalregulationoftheproteinkinaseasubunitsinsaccharomycescerevisiaeautoregulatoryroleofthekinaseaactivity AT rossisilviagraciela transcriptionalregulationoftheproteinkinaseasubunitsinsaccharomycescerevisiaeautoregulatoryroleofthekinaseaactivity |
| _version_ |
1768544338856378368 |