Benthic diatoms from Potter Cove, 25 de Mayo (King George) Island, Antarctica: Mucilage and glucan storage as a C-source for limpets
Biofilms were allowed to develop on ceramic tiles placed in closed containers on the shore of Potter Cove, 25 de Mayo (King George) Island. Water pumping from the cove inside the containers extended for 25 days. Diatoms were the dominant microalgae in these biofilms, which were removed from a set of...
| Publicado: |
2018
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18739652_v15_n_p39_Daglio http://hdl.handle.net/20.500.12110/paper_18739652_v15_n_p39_Daglio |
| Aporte de: |
| id |
paper:paper_18739652_v15_n_p39_Daglio |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_18739652_v15_n_p39_Daglio2023-06-08T16:30:02Z Benthic diatoms from Potter Cove, 25 de Mayo (King George) Island, Antarctica: Mucilage and glucan storage as a C-source for limpets 25 de Mayo (King George) Island Antarctica Benthic diatoms Feeding Gastropods Nacella concinna Potter Cove β-1,3-glucan benthos biofilm ceramics diatom feeding gastropod grazing identification method microalga mucilage polysaccharide snail storage Antarctica King George Island Potter Cove South Shetland Islands Bacillariophyta Gastropoda Nacella concinna Navicula Nitzschia pellucida Patellidae Pseudogomphonema Synedra Biofilms were allowed to develop on ceramic tiles placed in closed containers on the shore of Potter Cove, 25 de Mayo (King George) Island. Water pumping from the cove inside the containers extended for 25 days. Diatoms were the dominant microalgae in these biofilms, which were removed from a set of tiles to a) characterize the extracellular mucilage, b) carry out floristic determination and c) perform grazing experiments with the limpet Nacella concinna. Biofilms mucilaginous matrix consisted of proteins and carbohydrates. Room temperature aqueous extraction of the freeze-dried material rendered a fraction enriched in the storage glucan chrysolaminarin, its identity confirmed by methylation structural analyses. Hot water extracted products showed greater heterogeneity in monosaccharide composition, including glucose, mannose, galactose, fucose, xylose and rhamnose. Diatom identification revealed that Pseudogomphonema kamtschaticum was the dominant species followed by several Navicula species, Nitzschia pellucida and Synedra kerguelensis. Photographical survey of colonized tiles placed in glass flasks together with a specimen of Nacella concinna exhibited between 5 and 30% removal of the biofilms coverage after 24 h of exposure to the limpet, suggesting that EPS and chrysolaminarin constitute a C-source for the gastropod. © 2018 Elsevier B.V. and NIPR 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18739652_v15_n_p39_Daglio http://hdl.handle.net/20.500.12110/paper_18739652_v15_n_p39_Daglio |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
25 de Mayo (King George) Island Antarctica Benthic diatoms Feeding Gastropods Nacella concinna Potter Cove β-1,3-glucan benthos biofilm ceramics diatom feeding gastropod grazing identification method microalga mucilage polysaccharide snail storage Antarctica King George Island Potter Cove South Shetland Islands Bacillariophyta Gastropoda Nacella concinna Navicula Nitzschia pellucida Patellidae Pseudogomphonema Synedra |
| spellingShingle |
25 de Mayo (King George) Island Antarctica Benthic diatoms Feeding Gastropods Nacella concinna Potter Cove β-1,3-glucan benthos biofilm ceramics diatom feeding gastropod grazing identification method microalga mucilage polysaccharide snail storage Antarctica King George Island Potter Cove South Shetland Islands Bacillariophyta Gastropoda Nacella concinna Navicula Nitzschia pellucida Patellidae Pseudogomphonema Synedra Benthic diatoms from Potter Cove, 25 de Mayo (King George) Island, Antarctica: Mucilage and glucan storage as a C-source for limpets |
| topic_facet |
25 de Mayo (King George) Island Antarctica Benthic diatoms Feeding Gastropods Nacella concinna Potter Cove β-1,3-glucan benthos biofilm ceramics diatom feeding gastropod grazing identification method microalga mucilage polysaccharide snail storage Antarctica King George Island Potter Cove South Shetland Islands Bacillariophyta Gastropoda Nacella concinna Navicula Nitzschia pellucida Patellidae Pseudogomphonema Synedra |
| description |
Biofilms were allowed to develop on ceramic tiles placed in closed containers on the shore of Potter Cove, 25 de Mayo (King George) Island. Water pumping from the cove inside the containers extended for 25 days. Diatoms were the dominant microalgae in these biofilms, which were removed from a set of tiles to a) characterize the extracellular mucilage, b) carry out floristic determination and c) perform grazing experiments with the limpet Nacella concinna. Biofilms mucilaginous matrix consisted of proteins and carbohydrates. Room temperature aqueous extraction of the freeze-dried material rendered a fraction enriched in the storage glucan chrysolaminarin, its identity confirmed by methylation structural analyses. Hot water extracted products showed greater heterogeneity in monosaccharide composition, including glucose, mannose, galactose, fucose, xylose and rhamnose. Diatom identification revealed that Pseudogomphonema kamtschaticum was the dominant species followed by several Navicula species, Nitzschia pellucida and Synedra kerguelensis. Photographical survey of colonized tiles placed in glass flasks together with a specimen of Nacella concinna exhibited between 5 and 30% removal of the biofilms coverage after 24 h of exposure to the limpet, suggesting that EPS and chrysolaminarin constitute a C-source for the gastropod. © 2018 Elsevier B.V. and NIPR |
| title |
Benthic diatoms from Potter Cove, 25 de Mayo (King George) Island, Antarctica: Mucilage and glucan storage as a C-source for limpets |
| title_short |
Benthic diatoms from Potter Cove, 25 de Mayo (King George) Island, Antarctica: Mucilage and glucan storage as a C-source for limpets |
| title_full |
Benthic diatoms from Potter Cove, 25 de Mayo (King George) Island, Antarctica: Mucilage and glucan storage as a C-source for limpets |
| title_fullStr |
Benthic diatoms from Potter Cove, 25 de Mayo (King George) Island, Antarctica: Mucilage and glucan storage as a C-source for limpets |
| title_full_unstemmed |
Benthic diatoms from Potter Cove, 25 de Mayo (King George) Island, Antarctica: Mucilage and glucan storage as a C-source for limpets |
| title_sort |
benthic diatoms from potter cove, 25 de mayo (king george) island, antarctica: mucilage and glucan storage as a c-source for limpets |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18739652_v15_n_p39_Daglio http://hdl.handle.net/20.500.12110/paper_18739652_v15_n_p39_Daglio |
| _version_ |
1768544841475555328 |