Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers
Background: Chagas disease vectors (Hemiptera-Reduviidae) comprise more than 140 blood-sucking insect species of the Triatominae subfamily. The largest genus is Triatoma, subdivided in several complexes and subcomplexes according to morphology, ecology and genetic features. One of them is the sordid...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17563305_v8_n1_p_Panzera http://hdl.handle.net/20.500.12110/paper_17563305_v8_n1_p_Panzera |
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paper:paper_17563305_v8_n1_p_Panzera2023-06-08T16:28:54Z Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers Chagas disease vectors Cryptic species FISH Holocentric chromosomes Sordida subcomplex species Triatominae mitochondrial DNA cytochrome c oxidase genetic marker insect protein Argentina Article Bolivia Brazil chromosome identification DNA sequence gene sequence heterochromatin nonhuman nucleotide sequence Paraguay phenotype species differentiation species distribution Triatoma garciabesi Triatoma guasayana Triatoma sordida Triatoma sordida Argentina Triatoma sordida La Paz Triatoma sordida sensu stricto animal classification fluorescence in situ hybridization genetic marker genetics insect chromosome insect vector molecular genetics phylogeny Triatoma Animals Chromosomes, Insect Electron Transport Complex IV Genetic Markers In Situ Hybridization, Fluorescence Insect Proteins Insect Vectors Molecular Sequence Data Phylogeny Triatoma Background: Chagas disease vectors (Hemiptera-Reduviidae) comprise more than 140 blood-sucking insect species of the Triatominae subfamily. The largest genus is Triatoma, subdivided in several complexes and subcomplexes according to morphology, ecology and genetic features. One of them is the sordida subcomplex, involving four species: Triatoma sordida, T. guasayana, T. garciabesi and T. patagonica. Given the great morphological similarity of these species, their taxonomic identification, evolutionary relationships and population differentiation have been controversial for many years and even today remain under discussion. Methods: We simultaneously analyzed two chromosomal markers, C-heterochromatin distribution and 45S ribosomal genes chromosomal position, of 139 specimens from several sordida subcomplex populations from Argentina, Bolivia, Brazil and Paraguay, collected both in nature and from several established insectaries. Our results were compared with COI sequences deposited in GenBank. Results: We recognized five chromosomal taxa with putative hybrids, which each differ in at least one chromosome marker. Most of them present significant differences in their mtDNA sequences. Conclusion: The chromosomal taxa here show a significant chromosome differentiation involving changes in the C-heterochromatin content and in the ribosomal clusters position. This paper identifies several erroneously classified populations by morphological methods, delimits the geographical distribution of each taxon and proposes the existence of a new cryptic species, widely distributed in Argentina. We also suggest that sordida sibling species involve closely related as well as evolutionary distant species. Taxonomic status of each chromosomal taxon is discussed considering phenotypic and genetic results previously published. © 2015 Panzera et al. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17563305_v8_n1_p_Panzera http://hdl.handle.net/20.500.12110/paper_17563305_v8_n1_p_Panzera |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chagas disease vectors Cryptic species FISH Holocentric chromosomes Sordida subcomplex species Triatominae mitochondrial DNA cytochrome c oxidase genetic marker insect protein Argentina Article Bolivia Brazil chromosome identification DNA sequence gene sequence heterochromatin nonhuman nucleotide sequence Paraguay phenotype species differentiation species distribution Triatoma garciabesi Triatoma guasayana Triatoma sordida Triatoma sordida Argentina Triatoma sordida La Paz Triatoma sordida sensu stricto animal classification fluorescence in situ hybridization genetic marker genetics insect chromosome insect vector molecular genetics phylogeny Triatoma Animals Chromosomes, Insect Electron Transport Complex IV Genetic Markers In Situ Hybridization, Fluorescence Insect Proteins Insect Vectors Molecular Sequence Data Phylogeny Triatoma |
| spellingShingle |
Chagas disease vectors Cryptic species FISH Holocentric chromosomes Sordida subcomplex species Triatominae mitochondrial DNA cytochrome c oxidase genetic marker insect protein Argentina Article Bolivia Brazil chromosome identification DNA sequence gene sequence heterochromatin nonhuman nucleotide sequence Paraguay phenotype species differentiation species distribution Triatoma garciabesi Triatoma guasayana Triatoma sordida Triatoma sordida Argentina Triatoma sordida La Paz Triatoma sordida sensu stricto animal classification fluorescence in situ hybridization genetic marker genetics insect chromosome insect vector molecular genetics phylogeny Triatoma Animals Chromosomes, Insect Electron Transport Complex IV Genetic Markers In Situ Hybridization, Fluorescence Insect Proteins Insect Vectors Molecular Sequence Data Phylogeny Triatoma Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers |
| topic_facet |
Chagas disease vectors Cryptic species FISH Holocentric chromosomes Sordida subcomplex species Triatominae mitochondrial DNA cytochrome c oxidase genetic marker insect protein Argentina Article Bolivia Brazil chromosome identification DNA sequence gene sequence heterochromatin nonhuman nucleotide sequence Paraguay phenotype species differentiation species distribution Triatoma garciabesi Triatoma guasayana Triatoma sordida Triatoma sordida Argentina Triatoma sordida La Paz Triatoma sordida sensu stricto animal classification fluorescence in situ hybridization genetic marker genetics insect chromosome insect vector molecular genetics phylogeny Triatoma Animals Chromosomes, Insect Electron Transport Complex IV Genetic Markers In Situ Hybridization, Fluorescence Insect Proteins Insect Vectors Molecular Sequence Data Phylogeny Triatoma |
| description |
Background: Chagas disease vectors (Hemiptera-Reduviidae) comprise more than 140 blood-sucking insect species of the Triatominae subfamily. The largest genus is Triatoma, subdivided in several complexes and subcomplexes according to morphology, ecology and genetic features. One of them is the sordida subcomplex, involving four species: Triatoma sordida, T. guasayana, T. garciabesi and T. patagonica. Given the great morphological similarity of these species, their taxonomic identification, evolutionary relationships and population differentiation have been controversial for many years and even today remain under discussion. Methods: We simultaneously analyzed two chromosomal markers, C-heterochromatin distribution and 45S ribosomal genes chromosomal position, of 139 specimens from several sordida subcomplex populations from Argentina, Bolivia, Brazil and Paraguay, collected both in nature and from several established insectaries. Our results were compared with COI sequences deposited in GenBank. Results: We recognized five chromosomal taxa with putative hybrids, which each differ in at least one chromosome marker. Most of them present significant differences in their mtDNA sequences. Conclusion: The chromosomal taxa here show a significant chromosome differentiation involving changes in the C-heterochromatin content and in the ribosomal clusters position. This paper identifies several erroneously classified populations by morphological methods, delimits the geographical distribution of each taxon and proposes the existence of a new cryptic species, widely distributed in Argentina. We also suggest that sordida sibling species involve closely related as well as evolutionary distant species. Taxonomic status of each chromosomal taxon is discussed considering phenotypic and genetic results previously published. © 2015 Panzera et al. |
| title |
Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers |
| title_short |
Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers |
| title_full |
Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers |
| title_fullStr |
Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers |
| title_full_unstemmed |
Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers |
| title_sort |
cryptic speciation in the triatoma sordida subcomplex (hemiptera, reduviidae) revealed by chromosomal markers |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17563305_v8_n1_p_Panzera http://hdl.handle.net/20.500.12110/paper_17563305_v8_n1_p_Panzera |
| _version_ |
1768545437578428416 |