Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells
Introduction: Stromal-epithelial interactions mediate both breast development and breast cancer progression. In the present work, we evaluated the effects of conditioned media (CMs) of human adipose tissue explants from normal (hATN) and tumor (hATT) breast on proliferation, adhesion, migration and...
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paper:paper_1699048X_v15_n2_p124_PistoneCreydt2023-06-08T16:26:55Z Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells Pistone Creydt, Virginia Fletcher, Sabrina Johanna Giudice, Jimena Sacca, Paula Alejandra Calvo, Juan Carlos Breast epithelial cells Cancer Epithelial-stromal interactions Human breast adipose tissue gelatinase B adipose tissue article breast epithelium breast tumor cell adhesion cell migration cell proliferation controlled study enzyme activity enzyme assay explant human human cell human tissue molecular dynamics quantitative analysis tissue characterization tissue interaction tissue level tumor growth Adipose Tissue Breast Neoplasms Cell Adhesion Cell Movement Cell Proliferation Culture Media, Conditioned Epithelial Cells Female Humans Mammary Glands, Human Tumor Microenvironment Introduction: Stromal-epithelial interactions mediate both breast development and breast cancer progression. In the present work, we evaluated the effects of conditioned media (CMs) of human adipose tissue explants from normal (hATN) and tumor (hATT) breast on proliferation, adhesion, migration and metalloproteases activity on tumor (MCF-7 and IBH-7) and non-tumor (MCF-10A) human breast epithelial cell lines. Materials and methods: Human adipose tissues were obtained from patients and the conditioned medium from hATN and hATT collected after 24 h of incubation. MCF-10A, MCF-7 and IBH-7 cells were grown and incubated with CMs and proliferation and adhesion, as well as migration ability and metalloprotease activity, of epithelial cells after exposing cell cultures to hATN- or hATT-CMs were quantified. The statistical significance between different experimental conditions was evaluated by one-way ANOVA. Tukey′s post hoc tests were performed. Results: Tumor and non-tumor breast epithelial cells significantly increased their proliferation activity after 24 h of treatment with hATT-CMs compared to control-CMs. Furthermore, cellular adhesion of these two tumor cell lines was significantly lower with hATT-CMs than with hATN-CMs. Therefore, hATT-CMs seem to induce significantly lower expression or less activity of the components involved in cellular adhesion than hATN-CMs. In addition, hATT-CMs induced pro-MMP-9 and MMP-9 activity and increased the migration of MCF-7 and IBH-7 cells compared to hATN-CMs. Conclusions: We conclude that the microenvironment of the tumor interacts in a dynamic way with the mutated epithelium. This evidence leads to the possibility to modify the tumor behavior/phenotype through the regulation or modification of its microenvironment. We developed a model in which we obtained CMs from adipose tissue explants completely, either from normal or tumor breast. In this way, we studied the contribution of soluble factors independently of the possible effects of direct cell contact. © 2012 Federación de Sociedades Españolas de Oncología (FESEO). Fil:Pistone Creydt, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fletcher, S.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giudice, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sacca, P.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1699048X_v15_n2_p124_PistoneCreydt http://hdl.handle.net/20.500.12110/paper_1699048X_v15_n2_p124_PistoneCreydt |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Breast epithelial cells Cancer Epithelial-stromal interactions Human breast adipose tissue gelatinase B adipose tissue article breast epithelium breast tumor cell adhesion cell migration cell proliferation controlled study enzyme activity enzyme assay explant human human cell human tissue molecular dynamics quantitative analysis tissue characterization tissue interaction tissue level tumor growth Adipose Tissue Breast Neoplasms Cell Adhesion Cell Movement Cell Proliferation Culture Media, Conditioned Epithelial Cells Female Humans Mammary Glands, Human Tumor Microenvironment |
| spellingShingle |
Breast epithelial cells Cancer Epithelial-stromal interactions Human breast adipose tissue gelatinase B adipose tissue article breast epithelium breast tumor cell adhesion cell migration cell proliferation controlled study enzyme activity enzyme assay explant human human cell human tissue molecular dynamics quantitative analysis tissue characterization tissue interaction tissue level tumor growth Adipose Tissue Breast Neoplasms Cell Adhesion Cell Movement Cell Proliferation Culture Media, Conditioned Epithelial Cells Female Humans Mammary Glands, Human Tumor Microenvironment Pistone Creydt, Virginia Fletcher, Sabrina Johanna Giudice, Jimena Sacca, Paula Alejandra Calvo, Juan Carlos Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells |
| topic_facet |
Breast epithelial cells Cancer Epithelial-stromal interactions Human breast adipose tissue gelatinase B adipose tissue article breast epithelium breast tumor cell adhesion cell migration cell proliferation controlled study enzyme activity enzyme assay explant human human cell human tissue molecular dynamics quantitative analysis tissue characterization tissue interaction tissue level tumor growth Adipose Tissue Breast Neoplasms Cell Adhesion Cell Movement Cell Proliferation Culture Media, Conditioned Epithelial Cells Female Humans Mammary Glands, Human Tumor Microenvironment |
| description |
Introduction: Stromal-epithelial interactions mediate both breast development and breast cancer progression. In the present work, we evaluated the effects of conditioned media (CMs) of human adipose tissue explants from normal (hATN) and tumor (hATT) breast on proliferation, adhesion, migration and metalloproteases activity on tumor (MCF-7 and IBH-7) and non-tumor (MCF-10A) human breast epithelial cell lines. Materials and methods: Human adipose tissues were obtained from patients and the conditioned medium from hATN and hATT collected after 24 h of incubation. MCF-10A, MCF-7 and IBH-7 cells were grown and incubated with CMs and proliferation and adhesion, as well as migration ability and metalloprotease activity, of epithelial cells after exposing cell cultures to hATN- or hATT-CMs were quantified. The statistical significance between different experimental conditions was evaluated by one-way ANOVA. Tukey′s post hoc tests were performed. Results: Tumor and non-tumor breast epithelial cells significantly increased their proliferation activity after 24 h of treatment with hATT-CMs compared to control-CMs. Furthermore, cellular adhesion of these two tumor cell lines was significantly lower with hATT-CMs than with hATN-CMs. Therefore, hATT-CMs seem to induce significantly lower expression or less activity of the components involved in cellular adhesion than hATN-CMs. In addition, hATT-CMs induced pro-MMP-9 and MMP-9 activity and increased the migration of MCF-7 and IBH-7 cells compared to hATN-CMs. Conclusions: We conclude that the microenvironment of the tumor interacts in a dynamic way with the mutated epithelium. This evidence leads to the possibility to modify the tumor behavior/phenotype through the regulation or modification of its microenvironment. We developed a model in which we obtained CMs from adipose tissue explants completely, either from normal or tumor breast. In this way, we studied the contribution of soluble factors independently of the possible effects of direct cell contact. © 2012 Federación de Sociedades Españolas de Oncología (FESEO). |
| author |
Pistone Creydt, Virginia Fletcher, Sabrina Johanna Giudice, Jimena Sacca, Paula Alejandra Calvo, Juan Carlos |
| author_facet |
Pistone Creydt, Virginia Fletcher, Sabrina Johanna Giudice, Jimena Sacca, Paula Alejandra Calvo, Juan Carlos |
| author_sort |
Pistone Creydt, Virginia |
| title |
Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells |
| title_short |
Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells |
| title_full |
Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells |
| title_fullStr |
Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells |
| title_full_unstemmed |
Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells |
| title_sort |
human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1699048X_v15_n2_p124_PistoneCreydt http://hdl.handle.net/20.500.12110/paper_1699048X_v15_n2_p124_PistoneCreydt |
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1768542054158172160 |