Seminal plasma hexosaminidase in patients with normal and abnormal spermograms
Background: Glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. Although Hexosaminidase (Hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, Glucosidase is considered a marker for epididymal funct...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16806433_v13_n9_p541_MenendezHelman http://hdl.handle.net/20.500.12110/paper_16806433_v13_n9_p541_MenendezHelman |
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paper:paper_16806433_v13_n9_p541_MenendezHelman2023-06-08T16:26:39Z Seminal plasma hexosaminidase in patients with normal and abnormal spermograms Menéndez Helman, Renata Julia Glycosidases Hexosaminidase N-acetylglucosaminidase Semen Analysis Seminal plasma beta n acetylhexosaminidase beta n acetylhexosaminidase A glucosidase glutamic acid peroxidase phosphatase antibody specificity Article asthenospermia azoospermia cell motility cell structure cells by body anatomy controlled study correlation analysis cross-sectional study disease classification enzyme activity fertilization human human cell human tissue immunoblotting male protein expression round cell semen analysis seminal plasma spermatozoon abnormality spermiogram Western blotting asthenospermia azoospermia enzyme activity fluorometry polyacrylamide gel electrophoresis semen analysis seminal plasma spermatozoon density spermatozoon motility Background: Glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. Although Hexosaminidase (Hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, Glucosidase is considered a marker for epididymal function and azoospermia. Objective: The aim of this study was to determine Hex activity in seminal plasma from patients with normal and abnormal spermograms and analyze its correlation with seminal parameters. Materials and Methods: In this cross sectional study, seminal plasma from azoospermic, asthenozoospermic, teratozoospermic, and normozoospermic patients was analyzed for the activity of: total Hex, HexA isoform, and glucosidase. Besides, hexosamine levels were determined, and the amount of Hex was quantified by immunoblot with a specific antibody. Correlation of Hex activity with seminal parameters was also analyzed. Results: Hex activity, like glucosidase, was significantly reduced in azoospermic samples (44, 49, and 60% reduction for total Hex, HexA and glucosidase, respectively). A reduced amount of Hex in azoospermic samples was confirmed by western immunoblot. Hex activity was negatively correlated with round cells in azoospermic samples and positively correlated with motility in asthenozoospermic ones. Conclusion: The results suggested that Hex activity was reduced in azoospermic samples and this was due to a lower amount of enzyme. The correlation to seminal parameters related to particular pathologies suggests a possible relationship of Hex with fertilizing capacity. © 2015 Research and Clinical Center for Infertitlity. All rights reserved. Fil:Menendez-Helman, R.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16806433_v13_n9_p541_MenendezHelman http://hdl.handle.net/20.500.12110/paper_16806433_v13_n9_p541_MenendezHelman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Glycosidases Hexosaminidase N-acetylglucosaminidase Semen Analysis Seminal plasma beta n acetylhexosaminidase beta n acetylhexosaminidase A glucosidase glutamic acid peroxidase phosphatase antibody specificity Article asthenospermia azoospermia cell motility cell structure cells by body anatomy controlled study correlation analysis cross-sectional study disease classification enzyme activity fertilization human human cell human tissue immunoblotting male protein expression round cell semen analysis seminal plasma spermatozoon abnormality spermiogram Western blotting asthenospermia azoospermia enzyme activity fluorometry polyacrylamide gel electrophoresis semen analysis seminal plasma spermatozoon density spermatozoon motility |
| spellingShingle |
Glycosidases Hexosaminidase N-acetylglucosaminidase Semen Analysis Seminal plasma beta n acetylhexosaminidase beta n acetylhexosaminidase A glucosidase glutamic acid peroxidase phosphatase antibody specificity Article asthenospermia azoospermia cell motility cell structure cells by body anatomy controlled study correlation analysis cross-sectional study disease classification enzyme activity fertilization human human cell human tissue immunoblotting male protein expression round cell semen analysis seminal plasma spermatozoon abnormality spermiogram Western blotting asthenospermia azoospermia enzyme activity fluorometry polyacrylamide gel electrophoresis semen analysis seminal plasma spermatozoon density spermatozoon motility Menéndez Helman, Renata Julia Seminal plasma hexosaminidase in patients with normal and abnormal spermograms |
| topic_facet |
Glycosidases Hexosaminidase N-acetylglucosaminidase Semen Analysis Seminal plasma beta n acetylhexosaminidase beta n acetylhexosaminidase A glucosidase glutamic acid peroxidase phosphatase antibody specificity Article asthenospermia azoospermia cell motility cell structure cells by body anatomy controlled study correlation analysis cross-sectional study disease classification enzyme activity fertilization human human cell human tissue immunoblotting male protein expression round cell semen analysis seminal plasma spermatozoon abnormality spermiogram Western blotting asthenospermia azoospermia enzyme activity fluorometry polyacrylamide gel electrophoresis semen analysis seminal plasma spermatozoon density spermatozoon motility |
| description |
Background: Glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. Although Hexosaminidase (Hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, Glucosidase is considered a marker for epididymal function and azoospermia. Objective: The aim of this study was to determine Hex activity in seminal plasma from patients with normal and abnormal spermograms and analyze its correlation with seminal parameters. Materials and Methods: In this cross sectional study, seminal plasma from azoospermic, asthenozoospermic, teratozoospermic, and normozoospermic patients was analyzed for the activity of: total Hex, HexA isoform, and glucosidase. Besides, hexosamine levels were determined, and the amount of Hex was quantified by immunoblot with a specific antibody. Correlation of Hex activity with seminal parameters was also analyzed. Results: Hex activity, like glucosidase, was significantly reduced in azoospermic samples (44, 49, and 60% reduction for total Hex, HexA and glucosidase, respectively). A reduced amount of Hex in azoospermic samples was confirmed by western immunoblot. Hex activity was negatively correlated with round cells in azoospermic samples and positively correlated with motility in asthenozoospermic ones. Conclusion: The results suggested that Hex activity was reduced in azoospermic samples and this was due to a lower amount of enzyme. The correlation to seminal parameters related to particular pathologies suggests a possible relationship of Hex with fertilizing capacity. © 2015 Research and Clinical Center for Infertitlity. All rights reserved. |
| author |
Menéndez Helman, Renata Julia |
| author_facet |
Menéndez Helman, Renata Julia |
| author_sort |
Menéndez Helman, Renata Julia |
| title |
Seminal plasma hexosaminidase in patients with normal and abnormal spermograms |
| title_short |
Seminal plasma hexosaminidase in patients with normal and abnormal spermograms |
| title_full |
Seminal plasma hexosaminidase in patients with normal and abnormal spermograms |
| title_fullStr |
Seminal plasma hexosaminidase in patients with normal and abnormal spermograms |
| title_full_unstemmed |
Seminal plasma hexosaminidase in patients with normal and abnormal spermograms |
| title_sort |
seminal plasma hexosaminidase in patients with normal and abnormal spermograms |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16806433_v13_n9_p541_MenendezHelman http://hdl.handle.net/20.500.12110/paper_16806433_v13_n9_p541_MenendezHelman |
| work_keys_str_mv |
AT menendezhelmanrenatajulia seminalplasmahexosaminidaseinpatientswithnormalandabnormalspermograms |
| _version_ |
1768545574052691968 |