Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi

The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gβ subunit displays enhanced susceptibility to these pathogens. Gβ/AGB1 forms an obligate dimer with either one of the Arabidopsis Gγ subunits (γ1/AGG1 and γ2/AGG2)...

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Autores principales:	Fernández, Paula Virginia, Estevez, Jose Manuel
Publicado:	2012
Materias:	cell wall G-protein indole glucosinolates necrotropic fungi Plant immunity xylose Arabidopsis Fungi Plectosphaerella cucumerina
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16742052_v5_n1_p98_DelgadoCerezo http://hdl.handle.net/20.500.12110/paper_16742052_v5_n1_p98_DelgadoCerezo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_16742052_v5_n1_p98_DelgadoCerezo
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spelling	paper:paper_16742052_v5_n1_p98_DelgadoCerezo2023-06-08T16:26:24Z Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi Fernández, Paula Virginia Estevez, Jose Manuel cell wall G-protein indole glucosinolates necrotropic fungi Plant immunity xylose Arabidopsis Fungi Plectosphaerella cucumerina The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gβ subunit displays enhanced susceptibility to these pathogens. Gβ/AGB1 forms an obligate dimer with either one of the Arabidopsis Gγ subunits (γ1/AGG1 and γ2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulated genes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gβ and Gγ1/γ2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition. © 2011 The Author. Fil:Fernández, P.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16742052_v5_n1_p98_DelgadoCerezo http://hdl.handle.net/20.500.12110/paper_16742052_v5_n1_p98_DelgadoCerezo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cell wall G-protein indole glucosinolates necrotropic fungi Plant immunity xylose Arabidopsis Fungi Plectosphaerella cucumerina
spellingShingle	cell wall G-protein indole glucosinolates necrotropic fungi Plant immunity xylose Arabidopsis Fungi Plectosphaerella cucumerina Fernández, Paula Virginia Estevez, Jose Manuel Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi
topic_facet	cell wall G-protein indole glucosinolates necrotropic fungi Plant immunity xylose Arabidopsis Fungi Plectosphaerella cucumerina
description	The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gβ subunit displays enhanced susceptibility to these pathogens. Gβ/AGB1 forms an obligate dimer with either one of the Arabidopsis Gγ subunits (γ1/AGG1 and γ2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulated genes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gβ and Gγ1/γ2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition. © 2011 The Author.
author	Fernández, Paula Virginia Estevez, Jose Manuel
author_facet	Fernández, Paula Virginia Estevez, Jose Manuel
author_sort	Fernández, Paula Virginia
title	Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi
title_short	Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi
title_full	Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi
title_fullStr	Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi
title_full_unstemmed	Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi
title_sort	arabidopsis heterotrimeric g-protein regulates cell wall defense and resistance to necrotrophic fungi
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16742052_v5_n1_p98_DelgadoCerezo http://hdl.handle.net/20.500.12110/paper_16742052_v5_n1_p98_DelgadoCerezo
work_keys_str_mv	AT fernandezpaulavirginia arabidopsisheterotrimericgproteinregulatescellwalldefenseandresistancetonecrotrophicfungi AT estevezjosemanuel arabidopsisheterotrimericgproteinregulatescellwalldefenseandresistancetonecrotrophicfungi
_version_	1768544884772306944

Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi

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