Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae)
The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the H...
| Publicado: |
2013
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16603397_v11_n11_p4176_Guimaraes http://hdl.handle.net/20.500.12110/paper_16603397_v11_n11_p4176_Guimaraes |
| Aporte de: |
| id |
paper:paper_16603397_v11_n11_p4176_Guimaraes |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_16603397_v11_n11_p4176_Guimaraes2023-06-08T16:25:41Z Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae) Antiviral activity HSV-1 Marine sponge Petromica citrina Sulfate sterols acetic acid ethyl ester aciclovir alcohol antivirus agent butanol gD protein halistanol halistanol sulfate halistanol sulfate C ICP27 protein unclassified drug virus protein animal tissue antiviral activity article controlled study drug cytotoxicity drug isolation drug potentiation drug structure Herpes simplex virus 1 nonhuman Petromica citrina sponge (Porifera) Vero cell virus attachment virus entry virus particle virus replication virus strain 1-Butanol Animals Antiviral Agents Brazil Cell Line Cercopithecus aethiops Herpesvirus 1, Human Porifera Sterols Vero Cells Viral Plaque Assay The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2. © 2013 by the authors; licensee MDPI. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16603397_v11_n11_p4176_Guimaraes http://hdl.handle.net/20.500.12110/paper_16603397_v11_n11_p4176_Guimaraes |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antiviral activity HSV-1 Marine sponge Petromica citrina Sulfate sterols acetic acid ethyl ester aciclovir alcohol antivirus agent butanol gD protein halistanol halistanol sulfate halistanol sulfate C ICP27 protein unclassified drug virus protein animal tissue antiviral activity article controlled study drug cytotoxicity drug isolation drug potentiation drug structure Herpes simplex virus 1 nonhuman Petromica citrina sponge (Porifera) Vero cell virus attachment virus entry virus particle virus replication virus strain 1-Butanol Animals Antiviral Agents Brazil Cell Line Cercopithecus aethiops Herpesvirus 1, Human Porifera Sterols Vero Cells Viral Plaque Assay |
| spellingShingle |
Antiviral activity HSV-1 Marine sponge Petromica citrina Sulfate sterols acetic acid ethyl ester aciclovir alcohol antivirus agent butanol gD protein halistanol halistanol sulfate halistanol sulfate C ICP27 protein unclassified drug virus protein animal tissue antiviral activity article controlled study drug cytotoxicity drug isolation drug potentiation drug structure Herpes simplex virus 1 nonhuman Petromica citrina sponge (Porifera) Vero cell virus attachment virus entry virus particle virus replication virus strain 1-Butanol Animals Antiviral Agents Brazil Cell Line Cercopithecus aethiops Herpesvirus 1, Human Porifera Sterols Vero Cells Viral Plaque Assay Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae) |
| topic_facet |
Antiviral activity HSV-1 Marine sponge Petromica citrina Sulfate sterols acetic acid ethyl ester aciclovir alcohol antivirus agent butanol gD protein halistanol halistanol sulfate halistanol sulfate C ICP27 protein unclassified drug virus protein animal tissue antiviral activity article controlled study drug cytotoxicity drug isolation drug potentiation drug structure Herpes simplex virus 1 nonhuman Petromica citrina sponge (Porifera) Vero cell virus attachment virus entry virus particle virus replication virus strain 1-Butanol Animals Antiviral Agents Brazil Cell Line Cercopithecus aethiops Herpesvirus 1, Human Porifera Sterols Vero Cells Viral Plaque Assay |
| description |
The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2. © 2013 by the authors; licensee MDPI. |
| title |
Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae) |
| title_short |
Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae) |
| title_full |
Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae) |
| title_fullStr |
Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae) |
| title_full_unstemmed |
Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae) |
| title_sort |
anti hsv-1 activity of halistanol sulfate and halistanol sulfate c isolated from brazilian marine sponge petromica citrina (demospongiae) |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16603397_v11_n11_p4176_Guimaraes http://hdl.handle.net/20.500.12110/paper_16603397_v11_n11_p4176_Guimaraes |
| _version_ |
1768541579247616000 |