Optimal concentration of organic solvents to be used in the broth microdilution method to determine the antimicrobial activity of natural products against Paenibacillus larvae

American Foulbrood (AFB) is a bacterial disease, caused by Paenibacillus larvae, that affects honeybees (Apis mellifera). Alternative strategies to control AFB are based on the treatment of the beehives with antimicrobial natural substances such as extracts, essential oils and/or pure compounds from...

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Guardado en:
Detalles Bibliográficos
Publicado: 2017
Materias:
American foulbrood
Antimicrobial activity
Broth microdilution
Natural product
Organic solvent
Paenibacillus larvae
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16434439_v61_n1_p37_Cugnata
http://hdl.handle.net/20.500.12110/paper_16434439_v61_n1_p37_Cugnata
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_16434439_v61_n1_p37_Cugnata
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spelling paper:paper_16434439_v61_n1_p37_Cugnata2023-06-08T16:25:40Z Optimal concentration of organic solvents to be used in the broth microdilution method to determine the antimicrobial activity of natural products against Paenibacillus larvae American foulbrood Antimicrobial activity Broth microdilution Natural product Organic solvent Paenibacillus larvae American Foulbrood (AFB) is a bacterial disease, caused by Paenibacillus larvae, that affects honeybees (Apis mellifera). Alternative strategies to control AFB are based on the treatment of the beehives with antimicrobial natural substances such as extracts, essential oils and/or pure compounds from plants, honey by-products, bacteria and moulds. The broth microdilution method is currently one of the most widely used methods to determine the minimum inhibitory concentration (MIC) of a substance. In this regard, the fact that most natural products, due to their lipophilic nature, must be dissolved in organic solvents or their aqueous mixtures is an issue of major concern because the organic solvent becomes part of the dilution in the incubation medium, and therefore, can interfere with bacterial viability depending on its nature and concentration. A systematic study was carried out to determine by the broth microdilution method the MIC and the maximum non inhibitory concentration (MNIC) against P. larvae of the most common organic solvents used to extract or dissolve natural products, i.e. ethanol, methanol, acetonitrile, n-butanol, dimethylsulfoxide, and acidified hydromethanolic solutions. From the MIC and MNIC for each organic solvent, recommended maximum concentrations in contact with P. larvae were established: DMSO 5% (v/v), acetonitrile 7.5% (v/v), ethanol 7.5% (v/v), methanol 12% (v/v), n-butanol 1% (v/v), and methanol-water-acetic acid (1.25:98.71:0.04, v/v/v). © 2017, RESEARCH INST POMOLOGY FLORICULTURE. All rights reserved. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16434439_v61_n1_p37_Cugnata http://hdl.handle.net/20.500.12110/paper_16434439_v61_n1_p37_Cugnata
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic American foulbrood
Antimicrobial activity
Broth microdilution
Natural product
Organic solvent
Paenibacillus larvae
spellingShingle American foulbrood
Antimicrobial activity
Broth microdilution
Natural product
Organic solvent
Paenibacillus larvae
Optimal concentration of organic solvents to be used in the broth microdilution method to determine the antimicrobial activity of natural products against Paenibacillus larvae
topic_facet American foulbrood
Antimicrobial activity
Broth microdilution
Natural product
Organic solvent
Paenibacillus larvae
description American Foulbrood (AFB) is a bacterial disease, caused by Paenibacillus larvae, that affects honeybees (Apis mellifera). Alternative strategies to control AFB are based on the treatment of the beehives with antimicrobial natural substances such as extracts, essential oils and/or pure compounds from plants, honey by-products, bacteria and moulds. The broth microdilution method is currently one of the most widely used methods to determine the minimum inhibitory concentration (MIC) of a substance. In this regard, the fact that most natural products, due to their lipophilic nature, must be dissolved in organic solvents or their aqueous mixtures is an issue of major concern because the organic solvent becomes part of the dilution in the incubation medium, and therefore, can interfere with bacterial viability depending on its nature and concentration. A systematic study was carried out to determine by the broth microdilution method the MIC and the maximum non inhibitory concentration (MNIC) against P. larvae of the most common organic solvents used to extract or dissolve natural products, i.e. ethanol, methanol, acetonitrile, n-butanol, dimethylsulfoxide, and acidified hydromethanolic solutions. From the MIC and MNIC for each organic solvent, recommended maximum concentrations in contact with P. larvae were established: DMSO 5% (v/v), acetonitrile 7.5% (v/v), ethanol 7.5% (v/v), methanol 12% (v/v), n-butanol 1% (v/v), and methanol-water-acetic acid (1.25:98.71:0.04, v/v/v). © 2017, RESEARCH INST POMOLOGY FLORICULTURE. All rights reserved.
title Optimal concentration of organic solvents to be used in the broth microdilution method to determine the antimicrobial activity of natural products against Paenibacillus larvae
title_short Optimal concentration of organic solvents to be used in the broth microdilution method to determine the antimicrobial activity of natural products against Paenibacillus larvae
title_full Optimal concentration of organic solvents to be used in the broth microdilution method to determine the antimicrobial activity of natural products against Paenibacillus larvae
title_fullStr Optimal concentration of organic solvents to be used in the broth microdilution method to determine the antimicrobial activity of natural products against Paenibacillus larvae
title_full_unstemmed Optimal concentration of organic solvents to be used in the broth microdilution method to determine the antimicrobial activity of natural products against Paenibacillus larvae
title_sort optimal concentration of organic solvents to be used in the broth microdilution method to determine the antimicrobial activity of natural products against paenibacillus larvae
publishDate 2017
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16434439_v61_n1_p37_Cugnata
http://hdl.handle.net/20.500.12110/paper_16434439_v61_n1_p37_Cugnata
_version_ 1768545023632080896

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