Glyphosate-based herbicides with different adjuvants are more potent inhibitors of 3T3-L1 fibroblast proliferation and differentiation to adipocytes than glyphosate alone

Glyphosate-based herbicides are extensively used for weed control all over the world. Thus, it is important to investigate the putative toxic effects of these commercial formulations which contain not only glyphosate but also different adjuvants. 3T3-L1 fibroblasts are a useful tool in the study of...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Martini, Claudia Noemí, Gabrielli, Matías, Vila, María del Carmen
Publicado: 2016
Materias:
3T3-L1 fibroblasts
Adjuvants
Differentiation
Glyphosate
Glyphosate-based herbicides
Proliferation
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16185641_v25_n3_p607_Martini
http://hdl.handle.net/20.500.12110/paper_16185641_v25_n3_p607_Martini
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_16185641_v25_n3_p607_Martini
record_format dspace
spelling paper:paper_16185641_v25_n3_p607_Martini2023-06-08T16:25:35Z Glyphosate-based herbicides with different adjuvants are more potent inhibitors of 3T3-L1 fibroblast proliferation and differentiation to adipocytes than glyphosate alone Martini, Claudia Noemí Gabrielli, Matías Vila, María del Carmen 3T3-L1 fibroblasts Adjuvants Differentiation Glyphosate Glyphosate-based herbicides Proliferation Glyphosate-based herbicides are extensively used for weed control all over the world. Thus, it is important to investigate the putative toxic effects of these commercial formulations which contain not only glyphosate but also different adjuvants. 3T3-L1 fibroblasts are a useful tool in the study of adipocyte differentiation. We have previously reported that a commercial glyphosate formulation inhibits proliferation and differentiation in these cells. In the present investigation, we further evaluated the effect of different commercial glyphosate-based herbicides on 3T3-L1 fibroblast proliferation and differentiation and compared their effect with glyphosate itself. After treatment of 3T3-L1 fibroblasts with different concentrations of three glyphosate formulations or glyphosate itself, the increase in cell number or cytosolic lipid accumulation was determined. To evaluate the presence of polyethoxylated adjuvants, matrix-assisted laser desorption/ionization (MALDI) analysis of the three commercial glyphosate-based herbicides was also performed. We found that the three commercial formulations tested were more potent inhibitors of both physiological processes: proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts than glyphosate. We also found by MALDI analysis that the adjuvants were polyethoxylated in two of the formulations but not in the third one. It is well known the toxic effect of polyethoxylated adjuvants, but according to our results, non-polyethoxylated adjuvants may also contribute to the toxic effects. Our results further support the ability of glyphosate-based herbicides to disturb cellular physiology and highlight the importance of toxicological assessment of commercial formulations rather than glyphosate alone. © 2016, Springer-Verlag London. Fil:Martini, C.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gabrielli, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del Vila, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16185641_v25_n3_p607_Martini http://hdl.handle.net/20.500.12110/paper_16185641_v25_n3_p607_Martini
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 3T3-L1 fibroblasts
Adjuvants
Differentiation
Glyphosate
Glyphosate-based herbicides
Proliferation
spellingShingle 3T3-L1 fibroblasts
Adjuvants
Differentiation
Glyphosate
Glyphosate-based herbicides
Proliferation
Martini, Claudia Noemí
Gabrielli, Matías
Vila, María del Carmen
Glyphosate-based herbicides with different adjuvants are more potent inhibitors of 3T3-L1 fibroblast proliferation and differentiation to adipocytes than glyphosate alone
topic_facet 3T3-L1 fibroblasts
Adjuvants
Differentiation
Glyphosate
Glyphosate-based herbicides
Proliferation
description Glyphosate-based herbicides are extensively used for weed control all over the world. Thus, it is important to investigate the putative toxic effects of these commercial formulations which contain not only glyphosate but also different adjuvants. 3T3-L1 fibroblasts are a useful tool in the study of adipocyte differentiation. We have previously reported that a commercial glyphosate formulation inhibits proliferation and differentiation in these cells. In the present investigation, we further evaluated the effect of different commercial glyphosate-based herbicides on 3T3-L1 fibroblast proliferation and differentiation and compared their effect with glyphosate itself. After treatment of 3T3-L1 fibroblasts with different concentrations of three glyphosate formulations or glyphosate itself, the increase in cell number or cytosolic lipid accumulation was determined. To evaluate the presence of polyethoxylated adjuvants, matrix-assisted laser desorption/ionization (MALDI) analysis of the three commercial glyphosate-based herbicides was also performed. We found that the three commercial formulations tested were more potent inhibitors of both physiological processes: proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts than glyphosate. We also found by MALDI analysis that the adjuvants were polyethoxylated in two of the formulations but not in the third one. It is well known the toxic effect of polyethoxylated adjuvants, but according to our results, non-polyethoxylated adjuvants may also contribute to the toxic effects. Our results further support the ability of glyphosate-based herbicides to disturb cellular physiology and highlight the importance of toxicological assessment of commercial formulations rather than glyphosate alone. © 2016, Springer-Verlag London.
author Martini, Claudia Noemí
Gabrielli, Matías
Vila, María del Carmen
author_facet Martini, Claudia Noemí
Gabrielli, Matías
Vila, María del Carmen
author_sort Martini, Claudia Noemí
title Glyphosate-based herbicides with different adjuvants are more potent inhibitors of 3T3-L1 fibroblast proliferation and differentiation to adipocytes than glyphosate alone
title_short Glyphosate-based herbicides with different adjuvants are more potent inhibitors of 3T3-L1 fibroblast proliferation and differentiation to adipocytes than glyphosate alone
title_full Glyphosate-based herbicides with different adjuvants are more potent inhibitors of 3T3-L1 fibroblast proliferation and differentiation to adipocytes than glyphosate alone
title_fullStr Glyphosate-based herbicides with different adjuvants are more potent inhibitors of 3T3-L1 fibroblast proliferation and differentiation to adipocytes than glyphosate alone
title_full_unstemmed Glyphosate-based herbicides with different adjuvants are more potent inhibitors of 3T3-L1 fibroblast proliferation and differentiation to adipocytes than glyphosate alone
title_sort glyphosate-based herbicides with different adjuvants are more potent inhibitors of 3t3-l1 fibroblast proliferation and differentiation to adipocytes than glyphosate alone
publishDate 2016
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16185641_v25_n3_p607_Martini
http://hdl.handle.net/20.500.12110/paper_16185641_v25_n3_p607_Martini
work_keys_str_mv AT martiniclaudianoemi glyphosatebasedherbicideswithdifferentadjuvantsaremorepotentinhibitorsof3t3l1fibroblastproliferationanddifferentiationtoadipocytesthanglyphosatealone
AT gabriellimatias glyphosatebasedherbicideswithdifferentadjuvantsaremorepotentinhibitorsof3t3l1fibroblastproliferationanddifferentiationtoadipocytesthanglyphosatealone
AT vilamariadelcarmen glyphosatebasedherbicideswithdifferentadjuvantsaremorepotentinhibitorsof3t3l1fibroblastproliferationanddifferentiationtoadipocytesthanglyphosatealone
_version_ 1768546417024958464

Ejemplares similares