Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions

A strain of the lichen mycobiont isolated from a thallus of Parmotrema reticulatum was cultured axenically on different media. The morphology, anatomy, growth of the colonies, and metabolite production were studied. The isolated fungal colonies developed well and showed a remarkable morphogenetic ca...

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Autores principales: Fazio, Alejandra Teresa, Bertoni, María Delia Luisa, Adler, Monica Teresa, Ruíz, Laura Beatriz, Rosso, María Luciana, Maier, Marta Silvia
Publicado: 2009
Materias:
Atranorin
Lichen metabolites
Morphogenetic potential
Parmeliaceae
Triacylglycerides
Fungi
Parmotrema reticulatum
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1617416X_v8_n4_p359_Fazio
http://hdl.handle.net/20.500.12110/paper_1617416X_v8_n4_p359_Fazio
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_1617416X_v8_n4_p359_Fazio
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spelling paper:paper_1617416X_v8_n4_p359_Fazio2023-06-08T16:25:31Z Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions Fazio, Alejandra Teresa Bertoni, María Delia Luisa Adler, Monica Teresa Ruíz, Laura Beatriz Rosso, María Luciana Maier, Marta Silvia Atranorin Lichen metabolites Morphogenetic potential Parmeliaceae Triacylglycerides Fungi Parmeliaceae Parmotrema reticulatum A strain of the lichen mycobiont isolated from a thallus of Parmotrema reticulatum was cultured axenically on different media. The morphology, anatomy, growth of the colonies, and metabolite production were studied. The isolated fungal colonies developed well and showed a remarkable morphogenetic capacity on most of the assayed solid media, e. g., malt extract 2%-yeast extract 0.2% (MEYE), malt extract 1%-yeast extract 0.4%-sucrose 10% (MY10), and the original Lilly & Barnett medium (LB). The identity of the isolated fungus was confirmed by its ITS rDNA-sequence. Atranorin, the major cortical lichen depside, was produced when the colonies were grown over 5 and 10 months on solid LB medium, combined with a dessication treatment. Atranorin was identified by matching of UV spectra obtained from HPLC running and a reference substance in a spectrum library. Colonies grown on MEYE and MY10 with a dessication treatment did not produce any lichen secondary metabolite. Mycobionts grown for 5 months on solid MEYE without a dessication treatment produced triacylglycerides as the major metabolites, and the fatty acids were characterized as their methyl esters. Analysis by TLC and HPLC-DAD of extracts of colonies grown on LB and MY10 without dessication revealed that the typical secondary compounds of the natural lichen were not produced. The major metabolites of the natural lichen thallus were identified by chromatographic and spectroscopic methods. © German Mycological Society and Springer 2009. Fil:Fazio, A.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bertoni, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Adler, M.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruiz, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rosso, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maier, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1617416X_v8_n4_p359_Fazio http://hdl.handle.net/20.500.12110/paper_1617416X_v8_n4_p359_Fazio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Atranorin
Lichen metabolites
Morphogenetic potential
Parmeliaceae
Triacylglycerides
Fungi
Parmeliaceae
Parmotrema reticulatum
spellingShingle Atranorin
Lichen metabolites
Morphogenetic potential
Parmeliaceae
Triacylglycerides
Fungi
Parmeliaceae
Parmotrema reticulatum
Fazio, Alejandra Teresa
Bertoni, María Delia Luisa
Adler, Monica Teresa
Ruíz, Laura Beatriz
Rosso, María Luciana
Maier, Marta Silvia
Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions
topic_facet Atranorin
Lichen metabolites
Morphogenetic potential
Parmeliaceae
Triacylglycerides
Fungi
Parmeliaceae
Parmotrema reticulatum
description A strain of the lichen mycobiont isolated from a thallus of Parmotrema reticulatum was cultured axenically on different media. The morphology, anatomy, growth of the colonies, and metabolite production were studied. The isolated fungal colonies developed well and showed a remarkable morphogenetic capacity on most of the assayed solid media, e. g., malt extract 2%-yeast extract 0.2% (MEYE), malt extract 1%-yeast extract 0.4%-sucrose 10% (MY10), and the original Lilly & Barnett medium (LB). The identity of the isolated fungus was confirmed by its ITS rDNA-sequence. Atranorin, the major cortical lichen depside, was produced when the colonies were grown over 5 and 10 months on solid LB medium, combined with a dessication treatment. Atranorin was identified by matching of UV spectra obtained from HPLC running and a reference substance in a spectrum library. Colonies grown on MEYE and MY10 with a dessication treatment did not produce any lichen secondary metabolite. Mycobionts grown for 5 months on solid MEYE without a dessication treatment produced triacylglycerides as the major metabolites, and the fatty acids were characterized as their methyl esters. Analysis by TLC and HPLC-DAD of extracts of colonies grown on LB and MY10 without dessication revealed that the typical secondary compounds of the natural lichen were not produced. The major metabolites of the natural lichen thallus were identified by chromatographic and spectroscopic methods. © German Mycological Society and Springer 2009.
author Fazio, Alejandra Teresa
Bertoni, María Delia Luisa
Adler, Monica Teresa
Ruíz, Laura Beatriz
Rosso, María Luciana
Maier, Marta Silvia
author_facet Fazio, Alejandra Teresa
Bertoni, María Delia Luisa
Adler, Monica Teresa
Ruíz, Laura Beatriz
Rosso, María Luciana
Maier, Marta Silvia
author_sort Fazio, Alejandra Teresa
title Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions
title_short Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions
title_full Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions
title_fullStr Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions
title_full_unstemmed Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions
title_sort culture studies on the mycobiont isolated from parmotrema reticulatum (taylor) choisy: metabolite production under different conditions
publishDate 2009
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1617416X_v8_n4_p359_Fazio
http://hdl.handle.net/20.500.12110/paper_1617416X_v8_n4_p359_Fazio
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