Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers

The "algarrobo" [Prosopis chilensis (Molina) Stuntz] is a tree species that represents an important natural resource in arid and semi-arid regions of Argentina. In this paper, we analysed and compared the variability of 46 RAPD (Random Amplified Polymorphic DNA) loci with previous estimate...

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Detalles Bibliográficos
Publicado: 2010
Materias:
Environment factors
Genetic structure
Genetic variability
Homology
Isozyme
Prosopis chilensis
RAPD
RAPD bands
angiosperm
DNA
environmental conditions
environmental factor
genetic differentiation
genetic variation
heterozygosity
homology
population structure
Argentina
Prosopis
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16124669_v129_n6_p1011_Ferreyra
http://hdl.handle.net/20.500.12110/paper_16124669_v129_n6_p1011_Ferreyra
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_16124669_v129_n6_p1011_Ferreyra
record_format dspace
spelling paper:paper_16124669_v129_n6_p1011_Ferreyra2023-06-08T16:25:13Z Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers Environment factors Genetic structure Genetic variability Homology Isozyme Prosopis chilensis RAPD RAPD bands angiosperm DNA environmental conditions environmental factor genetic differentiation genetic variation heterozygosity homology population structure Argentina Prosopis Prosopis chilensis The "algarrobo" [Prosopis chilensis (Molina) Stuntz] is a tree species that represents an important natural resource in arid and semi-arid regions of Argentina. In this paper, we analysed and compared the variability of 46 RAPD (Random Amplified Polymorphic DNA) loci with previous estimates obtained from 12 isozyme markers in nine Argentinean populations of P. chilensis representative of the whole range of this species in Argentina. We evaluated the population structure and the existence of genetic variants associated with environmental variables. Expected heterozygosity (He) estimated from RAPD varied significantly among populations and regions. Hierarchical analysis of genetic variability (AMOVA) showed that most (88.1%) of the total diversity occurs within populations, the component among populations within regions (9.3%) was intermediate, while the between-region component was the lowest (2.6%). All three variance components were highly significant. The MDS plot from pair-wise ΦST matrix was consistent with the highly significant among-region differentiation indicated by the AMOVA. All 12 variable isozyme loci and 26 out of 46 RAPD loci showed significant or highly significant association with at least one geographic/climatic variable according to the stepwise multiple regression analysis. These results imply that the genetic differentiation among populations is better explained by environmental or biogeographical grounds than by geographical distances, suggesting gametic disequilibrium with loci responsible for the adaptation to particular environmental conditions. The information from RAPD markers would provide a relevant criterion to preserve genetic diversity in programmes for conservation and rationale use of this species. © 2010 Springer-Verlag. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16124669_v129_n6_p1011_Ferreyra http://hdl.handle.net/20.500.12110/paper_16124669_v129_n6_p1011_Ferreyra
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Environment factors
Genetic structure
Genetic variability
Homology
Isozyme
Prosopis chilensis
RAPD
RAPD bands
angiosperm
DNA
environmental conditions
environmental factor
genetic differentiation
genetic variation
heterozygosity
homology
population structure
Argentina
Prosopis
Prosopis chilensis
spellingShingle Environment factors
Genetic structure
Genetic variability
Homology
Isozyme
Prosopis chilensis
RAPD
RAPD bands
angiosperm
DNA
environmental conditions
environmental factor
genetic differentiation
genetic variation
heterozygosity
homology
population structure
Argentina
Prosopis
Prosopis chilensis
Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers
topic_facet Environment factors
Genetic structure
Genetic variability
Homology
Isozyme
Prosopis chilensis
RAPD
RAPD bands
angiosperm
DNA
environmental conditions
environmental factor
genetic differentiation
genetic variation
heterozygosity
homology
population structure
Argentina
Prosopis
Prosopis chilensis
description The "algarrobo" [Prosopis chilensis (Molina) Stuntz] is a tree species that represents an important natural resource in arid and semi-arid regions of Argentina. In this paper, we analysed and compared the variability of 46 RAPD (Random Amplified Polymorphic DNA) loci with previous estimates obtained from 12 isozyme markers in nine Argentinean populations of P. chilensis representative of the whole range of this species in Argentina. We evaluated the population structure and the existence of genetic variants associated with environmental variables. Expected heterozygosity (He) estimated from RAPD varied significantly among populations and regions. Hierarchical analysis of genetic variability (AMOVA) showed that most (88.1%) of the total diversity occurs within populations, the component among populations within regions (9.3%) was intermediate, while the between-region component was the lowest (2.6%). All three variance components were highly significant. The MDS plot from pair-wise ΦST matrix was consistent with the highly significant among-region differentiation indicated by the AMOVA. All 12 variable isozyme loci and 26 out of 46 RAPD loci showed significant or highly significant association with at least one geographic/climatic variable according to the stepwise multiple regression analysis. These results imply that the genetic differentiation among populations is better explained by environmental or biogeographical grounds than by geographical distances, suggesting gametic disequilibrium with loci responsible for the adaptation to particular environmental conditions. The information from RAPD markers would provide a relevant criterion to preserve genetic diversity in programmes for conservation and rationale use of this species. © 2010 Springer-Verlag.
title Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers
title_short Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers
title_full Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers
title_fullStr Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers
title_full_unstemmed Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers
title_sort adaptive genetic diversity and population structure of the "algarrobo" [prosopis chilensis (molina) stuntz] analysed by rapd and isozyme markers
publishDate 2010
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16124669_v129_n6_p1011_Ferreyra
http://hdl.handle.net/20.500.12110/paper_16124669_v129_n6_p1011_Ferreyra
_version_ 1768545619573473280

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