Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds

We have developed a spatial light modulator (SLM) based microscope that uses diffraction to shape the incoming two-photon laser source to any arbitrary light pattern. This allows the simultaneous imaging or photostimulation of different regions of a sample with three-dimensional precision at high fr...

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Autor principal: Etchenique, Roberto
Publicado: 2010
Materias:
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16057422_v7548_n_p_Peterka
http://hdl.handle.net/20.500.12110/paper_16057422_v7548_n_p_Peterka
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spelling paper:paper_16057422_v7548_n_p_Peterka2023-06-08T16:25:02Z Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds Etchenique, Roberto adaptive optics imaging microscope neuron Ruthenium Spatial light modulator two-photon uncaging Dimensional precision High frame rate imaging Light patterns Neuromodulators Neuronal control Photostimulation Ruthenium bipyridine Simultaneous imaging Spatial light modulators Two photon uncaging Light Light modulation Microscopes Photons Ruthenium Ruthenium compounds Transition metal compounds Light modulators We have developed a spatial light modulator (SLM) based microscope that uses diffraction to shape the incoming two-photon laser source to any arbitrary light pattern. This allows the simultaneous imaging or photostimulation of different regions of a sample with three-dimensional precision at high frame rates. Additionally, we have combined this microscope with a new class of two photon active neuromodulators with Ruthenium BiPyridine (RuBi) based cages that offer great flexibility for neuronal control. © 2010 Copyright SPIE - The International Society for Optical Engineering. Fil:Etchenique, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16057422_v7548_n_p_Peterka http://hdl.handle.net/20.500.12110/paper_16057422_v7548_n_p_Peterka
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic adaptive optics
imaging
microscope
neuron
Ruthenium
Spatial light modulator
two-photon
uncaging
Dimensional precision
High frame rate
imaging
Light patterns
Neuromodulators
Neuronal control
Photostimulation
Ruthenium bipyridine
Simultaneous imaging
Spatial light modulators
Two photon
uncaging
Light
Light modulation
Microscopes
Photons
Ruthenium
Ruthenium compounds
Transition metal compounds
Light modulators
spellingShingle adaptive optics
imaging
microscope
neuron
Ruthenium
Spatial light modulator
two-photon
uncaging
Dimensional precision
High frame rate
imaging
Light patterns
Neuromodulators
Neuronal control
Photostimulation
Ruthenium bipyridine
Simultaneous imaging
Spatial light modulators
Two photon
uncaging
Light
Light modulation
Microscopes
Photons
Ruthenium
Ruthenium compounds
Transition metal compounds
Light modulators
Etchenique, Roberto
Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds
topic_facet adaptive optics
imaging
microscope
neuron
Ruthenium
Spatial light modulator
two-photon
uncaging
Dimensional precision
High frame rate
imaging
Light patterns
Neuromodulators
Neuronal control
Photostimulation
Ruthenium bipyridine
Simultaneous imaging
Spatial light modulators
Two photon
uncaging
Light
Light modulation
Microscopes
Photons
Ruthenium
Ruthenium compounds
Transition metal compounds
Light modulators
description We have developed a spatial light modulator (SLM) based microscope that uses diffraction to shape the incoming two-photon laser source to any arbitrary light pattern. This allows the simultaneous imaging or photostimulation of different regions of a sample with three-dimensional precision at high frame rates. Additionally, we have combined this microscope with a new class of two photon active neuromodulators with Ruthenium BiPyridine (RuBi) based cages that offer great flexibility for neuronal control. © 2010 Copyright SPIE - The International Society for Optical Engineering.
author Etchenique, Roberto
author_facet Etchenique, Roberto
author_sort Etchenique, Roberto
title Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds
title_short Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds
title_full Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds
title_fullStr Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds
title_full_unstemmed Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds
title_sort fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds
publishDate 2010
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16057422_v7548_n_p_Peterka
http://hdl.handle.net/20.500.12110/paper_16057422_v7548_n_p_Peterka
work_keys_str_mv AT etcheniqueroberto fasttwophotonneuronalimagingandcontrolusingaspatiallightmodulatorandrutheniumcompounds
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