Responses of Living Organisms to Freezing and Drying: Potential Applications in Food Technology

Many sugars are involved in the preservation of living organisms. Under thermal or hydric stress conditions, spores, yeasts, and microscopic animals accumulate trehalose, whereas pollen, plant seeds, and resurrection plants synthesize sucrose and oligosaccharides such as raffinose and stachyose. The...

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Detalles Bibliográficos
Publicado:	2011
Materias:	Freeze Avoidance Recalcitrant Seed Subzero Temperature Supercooling Point Thermal Hysteresis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15710297_v_n_p553_delBuera http://hdl.handle.net/20.500.12110/paper_15710297_v_n_p553_delBuera
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15710297_v_n_p553_delBuera
record_format	dspace
spelling	paper:paper_15710297_v_n_p553_delBuera2023-06-08T16:24:20Z Responses of Living Organisms to Freezing and Drying: Potential Applications in Food Technology Freeze Avoidance Recalcitrant Seed Subzero Temperature Supercooling Point Thermal Hysteresis Many sugars are involved in the preservation of living organisms. Under thermal or hydric stress conditions, spores, yeasts, and microscopic animals accumulate trehalose, whereas pollen, plant seeds, and resurrection plants synthesize sucrose and oligosaccharides such as raffinose and stachyose. These solutes also have proved to provide stabilization of dried or frozen labile biomolecules in vitro and have potential technological applications for the preservation of special food ingredients, which is obviously of enormous economical importance. The protectants promote the formation of amorphous, glassy systems, inhibit crystallization, and influence the kinetics of reactions responsible for deterioration during storage. There is evidence, however, that the maintenance of a glassy structure is not the only factor controlling biomolecule stability. Anhydrobiotic engineering aims to confer desiccation tolerance on otherwise sensitive living organisms by adopting the strategies of anhydrobiosis, and a large number of genes with a potential role in drought tolerance have been described. Other nature-based methods to protect biomolecules in frozen environments are the manipulation of ice-nucleating agents (INAs) (present in several microorganisms and lichen species) and antifreeze components (characteristic of some Antarctic fish). The former catalyze ice formation at relative warm subfreezing temperatures, and antifreeze polymers act by surface interactions. Cryo and dehydropreservation of biomolecules, which is of technological value, is frequently developed on an empirical basis. In this chapter, how the physical and chemical mechanisms by which anhydrobiotic organisms can tolerate extreme conditions may allow establishing stabilization protocols on a scientific basis is shown. © 2010, Springer New York. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15710297_v_n_p553_delBuera http://hdl.handle.net/20.500.12110/paper_15710297_v_n_p553_delBuera
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Freeze Avoidance Recalcitrant Seed Subzero Temperature Supercooling Point Thermal Hysteresis
spellingShingle	Freeze Avoidance Recalcitrant Seed Subzero Temperature Supercooling Point Thermal Hysteresis Responses of Living Organisms to Freezing and Drying: Potential Applications in Food Technology
topic_facet	Freeze Avoidance Recalcitrant Seed Subzero Temperature Supercooling Point Thermal Hysteresis
description	Many sugars are involved in the preservation of living organisms. Under thermal or hydric stress conditions, spores, yeasts, and microscopic animals accumulate trehalose, whereas pollen, plant seeds, and resurrection plants synthesize sucrose and oligosaccharides such as raffinose and stachyose. These solutes also have proved to provide stabilization of dried or frozen labile biomolecules in vitro and have potential technological applications for the preservation of special food ingredients, which is obviously of enormous economical importance. The protectants promote the formation of amorphous, glassy systems, inhibit crystallization, and influence the kinetics of reactions responsible for deterioration during storage. There is evidence, however, that the maintenance of a glassy structure is not the only factor controlling biomolecule stability. Anhydrobiotic engineering aims to confer desiccation tolerance on otherwise sensitive living organisms by adopting the strategies of anhydrobiosis, and a large number of genes with a potential role in drought tolerance have been described. Other nature-based methods to protect biomolecules in frozen environments are the manipulation of ice-nucleating agents (INAs) (present in several microorganisms and lichen species) and antifreeze components (characteristic of some Antarctic fish). The former catalyze ice formation at relative warm subfreezing temperatures, and antifreeze polymers act by surface interactions. Cryo and dehydropreservation of biomolecules, which is of technological value, is frequently developed on an empirical basis. In this chapter, how the physical and chemical mechanisms by which anhydrobiotic organisms can tolerate extreme conditions may allow establishing stabilization protocols on a scientific basis is shown. © 2010, Springer New York.
title	Responses of Living Organisms to Freezing and Drying: Potential Applications in Food Technology
title_short	Responses of Living Organisms to Freezing and Drying: Potential Applications in Food Technology
title_full	Responses of Living Organisms to Freezing and Drying: Potential Applications in Food Technology
title_fullStr	Responses of Living Organisms to Freezing and Drying: Potential Applications in Food Technology
title_full_unstemmed	Responses of Living Organisms to Freezing and Drying: Potential Applications in Food Technology
title_sort	responses of living organisms to freezing and drying: potential applications in food technology
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15710297_v_n_p553_delBuera http://hdl.handle.net/20.500.12110/paper_15710297_v_n_p553_delBuera
_version_	1768543102855806976

Responses of Living Organisms to Freezing and Drying: Potential Applications in Food Technology

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