IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis

Protective immunity against Mycobacterium tuberculosis (Mtb) requires IFNG. Besides, IFNG-mediated induction of autophagy suppresses survival of virulent Mtb in macrophage cell lines. We investigated the contribution of autophagy to the defense against Mtb antigen (Mtb-Ag) in cells from tuberculosis...

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Autores principales:	Peña, Delfina, Bigi, Fabiana, García, Verónica Edith
Publicado:	2014
Materias:	Autophagy Cytokines Defense IFNG Immune response Patients Tuberculosis bacterial antigen bafilomycin A1 CD14 antigen gamma interferon receptor gamma interferon Mycobacterium tuberculosis antigens adaptive immunity adherent cell Article autophagosome autophagy bacterial strain CD3+ T lymphocyte cellular immunity confocal microscopy controlled study cytokine production cytokine release dendritic cell densitometry enzyme linked immunosorbent assay flow cytometry fluorescence activated cell sorting human human cell immunofluorescence innate immunity macrophage monocyte Mycobacterium tuberculosis peripheral blood mononuclear cell treatment response tuberculosis Western blotting drug effects female immunology male metabolism microbiology Th1 cell Antigens, Bacterial Female Humans Interferon-gamma Macrophages Male Th1 Cells
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15548627_v10_n12_p2109_Rovetta http://hdl.handle.net/20.500.12110/paper_15548627_v10_n12_p2109_Rovetta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15548627_v10_n12_p2109_Rovetta
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spelling	paper:paper_15548627_v10_n12_p2109_Rovetta2023-06-08T16:23:13Z IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis Peña, Delfina Bigi, Fabiana García, Verónica Edith Autophagy Cytokines Defense IFNG Immune response Patients Tuberculosis bacterial antigen bafilomycin A1 CD14 antigen gamma interferon receptor bacterial antigen gamma interferon Mycobacterium tuberculosis antigens adaptive immunity adherent cell Article autophagosome autophagy bacterial strain CD3+ T lymphocyte cellular immunity confocal microscopy controlled study cytokine production cytokine release dendritic cell densitometry enzyme linked immunosorbent assay flow cytometry fluorescence activated cell sorting human human cell immunofluorescence innate immunity macrophage monocyte Mycobacterium tuberculosis peripheral blood mononuclear cell treatment response tuberculosis Western blotting autophagy drug effects female immunology male metabolism microbiology Mycobacterium tuberculosis Th1 cell tuberculosis Mycobacterium tuberculosis Antigens, Bacterial Antigens, Bacterial Autophagy Autophagy Female Female Humans Humans Interferon-gamma Interferon-gamma Macrophages Macrophages Male Male Mycobacterium tuberculosis Mycobacterium tuberculosis Th1 Cells Th1 Cells Tuberculosis Tuberculosis Protective immunity against Mycobacterium tuberculosis (Mtb) requires IFNG. Besides, IFNG-mediated induction of autophagy suppresses survival of virulent Mtb in macrophage cell lines. We investigated the contribution of autophagy to the defense against Mtb antigen (Mtb-Ag) in cells from tuberculosis patients and healthy donors (HD). Patients were classified as high responders (HR) if their T cells produced significant IFNG against Mtb-Ag; and low responders (LR) when patients showed weak or no T cell responses to Mtb-Ag. The highest autophagy levels were detected in HD cells whereas the lowest quantities were observed in LR patients. Interestingly, upon Mtb-Ag stimulation, we detected a positive correlation between IFNG and MAP1LC3B-II/LC3-II levels. Actually, blockage of Mtb-Ag-induced IFNG markedly reduced autophagy in HR patients whereas addition of limited amounts of IFNG significantly increased autophagy in LR patients. Therefore, autophagy collaborates with human immune responses against Mtb in close association with specific IFNG secreted against the pathogen. © 2014 Taylor & Francis Group, LLC. Fil:Peña, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bigi, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:García, V.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15548627_v10_n12_p2109_Rovetta http://hdl.handle.net/20.500.12110/paper_15548627_v10_n12_p2109_Rovetta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Autophagy Cytokines Defense IFNG Immune response Patients Tuberculosis bacterial antigen bafilomycin A1 CD14 antigen gamma interferon receptor bacterial antigen gamma interferon Mycobacterium tuberculosis antigens adaptive immunity adherent cell Article autophagosome autophagy bacterial strain CD3+ T lymphocyte cellular immunity confocal microscopy controlled study cytokine production cytokine release dendritic cell densitometry enzyme linked immunosorbent assay flow cytometry fluorescence activated cell sorting human human cell immunofluorescence innate immunity macrophage monocyte Mycobacterium tuberculosis peripheral blood mononuclear cell treatment response tuberculosis Western blotting autophagy drug effects female immunology male metabolism microbiology Mycobacterium tuberculosis Th1 cell tuberculosis Mycobacterium tuberculosis Antigens, Bacterial Antigens, Bacterial Autophagy Autophagy Female Female Humans Humans Interferon-gamma Interferon-gamma Macrophages Macrophages Male Male Mycobacterium tuberculosis Mycobacterium tuberculosis Th1 Cells Th1 Cells Tuberculosis Tuberculosis
spellingShingle	Autophagy Cytokines Defense IFNG Immune response Patients Tuberculosis bacterial antigen bafilomycin A1 CD14 antigen gamma interferon receptor bacterial antigen gamma interferon Mycobacterium tuberculosis antigens adaptive immunity adherent cell Article autophagosome autophagy bacterial strain CD3+ T lymphocyte cellular immunity confocal microscopy controlled study cytokine production cytokine release dendritic cell densitometry enzyme linked immunosorbent assay flow cytometry fluorescence activated cell sorting human human cell immunofluorescence innate immunity macrophage monocyte Mycobacterium tuberculosis peripheral blood mononuclear cell treatment response tuberculosis Western blotting autophagy drug effects female immunology male metabolism microbiology Mycobacterium tuberculosis Th1 cell tuberculosis Mycobacterium tuberculosis Antigens, Bacterial Antigens, Bacterial Autophagy Autophagy Female Female Humans Humans Interferon-gamma Interferon-gamma Macrophages Macrophages Male Male Mycobacterium tuberculosis Mycobacterium tuberculosis Th1 Cells Th1 Cells Tuberculosis Tuberculosis Peña, Delfina Bigi, Fabiana García, Verónica Edith IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis
topic_facet	Autophagy Cytokines Defense IFNG Immune response Patients Tuberculosis bacterial antigen bafilomycin A1 CD14 antigen gamma interferon receptor bacterial antigen gamma interferon Mycobacterium tuberculosis antigens adaptive immunity adherent cell Article autophagosome autophagy bacterial strain CD3+ T lymphocyte cellular immunity confocal microscopy controlled study cytokine production cytokine release dendritic cell densitometry enzyme linked immunosorbent assay flow cytometry fluorescence activated cell sorting human human cell immunofluorescence innate immunity macrophage monocyte Mycobacterium tuberculosis peripheral blood mononuclear cell treatment response tuberculosis Western blotting autophagy drug effects female immunology male metabolism microbiology Mycobacterium tuberculosis Th1 cell tuberculosis Mycobacterium tuberculosis Antigens, Bacterial Antigens, Bacterial Autophagy Autophagy Female Female Humans Humans Interferon-gamma Interferon-gamma Macrophages Macrophages Male Male Mycobacterium tuberculosis Mycobacterium tuberculosis Th1 Cells Th1 Cells Tuberculosis Tuberculosis
description	Protective immunity against Mycobacterium tuberculosis (Mtb) requires IFNG. Besides, IFNG-mediated induction of autophagy suppresses survival of virulent Mtb in macrophage cell lines. We investigated the contribution of autophagy to the defense against Mtb antigen (Mtb-Ag) in cells from tuberculosis patients and healthy donors (HD). Patients were classified as high responders (HR) if their T cells produced significant IFNG against Mtb-Ag; and low responders (LR) when patients showed weak or no T cell responses to Mtb-Ag. The highest autophagy levels were detected in HD cells whereas the lowest quantities were observed in LR patients. Interestingly, upon Mtb-Ag stimulation, we detected a positive correlation between IFNG and MAP1LC3B-II/LC3-II levels. Actually, blockage of Mtb-Ag-induced IFNG markedly reduced autophagy in HR patients whereas addition of limited amounts of IFNG significantly increased autophagy in LR patients. Therefore, autophagy collaborates with human immune responses against Mtb in close association with specific IFNG secreted against the pathogen. © 2014 Taylor & Francis Group, LLC.
author	Peña, Delfina Bigi, Fabiana García, Verónica Edith
author_facet	Peña, Delfina Bigi, Fabiana García, Verónica Edith
author_sort	Peña, Delfina
title	IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis
title_short	IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis
title_full	IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis
title_fullStr	IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis
title_full_unstemmed	IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis
title_sort	ifng-mediated immune responses enhance autophagy against mycobacterium tuberculosis antigens in patients with active tuberculosis
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15548627_v10_n12_p2109_Rovetta http://hdl.handle.net/20.500.12110/paper_15548627_v10_n12_p2109_Rovetta
work_keys_str_mv	AT penadelfina ifngmediatedimmuneresponsesenhanceautophagyagainstmycobacteriumtuberculosisantigensinpatientswithactivetuberculosis AT bigifabiana ifngmediatedimmuneresponsesenhanceautophagyagainstmycobacteriumtuberculosisantigensinpatientswithactivetuberculosis AT garciaveronicaedith ifngmediatedimmuneresponsesenhanceautophagyagainstmycobacteriumtuberculosisantigensinpatientswithactivetuberculosis
_version_	1768542709598912512

IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis

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