Gene regulatory network architecture in different developmental contexts influences the genetic basis of morphological evolution
Convergent phenotypic evolution is often caused by recurrent changes at particular nodes in the underlying gene regulatory networks (GRNs). The genes at such evolutionary ‘hotspots’ are thought to maximally affect the phenotype with minimal pleiotropic consequences. This has led to the suggestion th...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15537390_v14_n5_p_Kittelmann http://hdl.handle.net/20.500.12110/paper_15537390_v14_n5_p_Kittelmann |
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paper:paper_15537390_v14_n5_p_Kittelmann2023-06-08T16:23:11Z Gene regulatory network architecture in different developmental contexts influences the genetic basis of morphological evolution microRNA microRNA 92a transcription factor transcription factor shavenbaby unclassified drug DNA binding protein Drosophila protein microRNA MIRN92 microRNA, Drosophila ovo protein, Drosophila transcription factor animal tissue Article controlled study convergent evolution Drosophila melanogaster ectopic expression embryo enhancer region exon femur gene expression gene regulatory network gene repression leg morphological adaptation nonhuman pupa reporter gene repressor gene RNA sequence trichome animal animal structures classification gene expression regulation genetics growth, development and aging larva metabolism molecular evolution mutation transgenic animal Animal Structures Animals Animals, Genetically Modified DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Evolution, Molecular Gene Expression Regulation, Developmental Gene Regulatory Networks Larva MicroRNAs Mutation Transcription Factors Convergent phenotypic evolution is often caused by recurrent changes at particular nodes in the underlying gene regulatory networks (GRNs). The genes at such evolutionary ‘hotspots’ are thought to maximally affect the phenotype with minimal pleiotropic consequences. This has led to the suggestion that if a GRN is understood in sufficient detail, the path of evolution may be predictable. The repeated evolutionary loss of larval trichomes among Drosophila species is caused by the loss of shavenbaby (svb) expression. svb is also required for development of leg trichomes, but the evolutionary gain of trichomes in the ‘naked valley’ on T2 femurs in Drosophila melanogaster is caused by reduced microRNA-92a (miR-92a) expression rather than changes in svb. We compared the expression and function of components between the larval and leg trichome GRNs to investigate why the genetic basis of trichome pattern evolution differs in these developmental contexts. We found key differences between the two networks in both the genes employed, and in the regulation and function of common genes. These differences in the GRNs reveal why mutations in svb are unlikely to contribute to leg trichome evolution and how instead miR-92a represents the key evolutionary switch in this context. Our work shows that variability in GRNs across different developmental contexts, as well as whether a morphological feature is lost versus gained, influence the nodes at which a GRN evolves to cause morphological change. Therefore, our findings have important implications for understanding the pathways and predictability of evolution. © 2018 Kittelmann et al. http://creativecommons.org/licenses/by/4.0/ 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15537390_v14_n5_p_Kittelmann http://hdl.handle.net/20.500.12110/paper_15537390_v14_n5_p_Kittelmann |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
microRNA microRNA 92a transcription factor transcription factor shavenbaby unclassified drug DNA binding protein Drosophila protein microRNA MIRN92 microRNA, Drosophila ovo protein, Drosophila transcription factor animal tissue Article controlled study convergent evolution Drosophila melanogaster ectopic expression embryo enhancer region exon femur gene expression gene regulatory network gene repression leg morphological adaptation nonhuman pupa reporter gene repressor gene RNA sequence trichome animal animal structures classification gene expression regulation genetics growth, development and aging larva metabolism molecular evolution mutation transgenic animal Animal Structures Animals Animals, Genetically Modified DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Evolution, Molecular Gene Expression Regulation, Developmental Gene Regulatory Networks Larva MicroRNAs Mutation Transcription Factors |
| spellingShingle |
microRNA microRNA 92a transcription factor transcription factor shavenbaby unclassified drug DNA binding protein Drosophila protein microRNA MIRN92 microRNA, Drosophila ovo protein, Drosophila transcription factor animal tissue Article controlled study convergent evolution Drosophila melanogaster ectopic expression embryo enhancer region exon femur gene expression gene regulatory network gene repression leg morphological adaptation nonhuman pupa reporter gene repressor gene RNA sequence trichome animal animal structures classification gene expression regulation genetics growth, development and aging larva metabolism molecular evolution mutation transgenic animal Animal Structures Animals Animals, Genetically Modified DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Evolution, Molecular Gene Expression Regulation, Developmental Gene Regulatory Networks Larva MicroRNAs Mutation Transcription Factors Gene regulatory network architecture in different developmental contexts influences the genetic basis of morphological evolution |
| topic_facet |
microRNA microRNA 92a transcription factor transcription factor shavenbaby unclassified drug DNA binding protein Drosophila protein microRNA MIRN92 microRNA, Drosophila ovo protein, Drosophila transcription factor animal tissue Article controlled study convergent evolution Drosophila melanogaster ectopic expression embryo enhancer region exon femur gene expression gene regulatory network gene repression leg morphological adaptation nonhuman pupa reporter gene repressor gene RNA sequence trichome animal animal structures classification gene expression regulation genetics growth, development and aging larva metabolism molecular evolution mutation transgenic animal Animal Structures Animals Animals, Genetically Modified DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Evolution, Molecular Gene Expression Regulation, Developmental Gene Regulatory Networks Larva MicroRNAs Mutation Transcription Factors |
| description |
Convergent phenotypic evolution is often caused by recurrent changes at particular nodes in the underlying gene regulatory networks (GRNs). The genes at such evolutionary ‘hotspots’ are thought to maximally affect the phenotype with minimal pleiotropic consequences. This has led to the suggestion that if a GRN is understood in sufficient detail, the path of evolution may be predictable. The repeated evolutionary loss of larval trichomes among Drosophila species is caused by the loss of shavenbaby (svb) expression. svb is also required for development of leg trichomes, but the evolutionary gain of trichomes in the ‘naked valley’ on T2 femurs in Drosophila melanogaster is caused by reduced microRNA-92a (miR-92a) expression rather than changes in svb. We compared the expression and function of components between the larval and leg trichome GRNs to investigate why the genetic basis of trichome pattern evolution differs in these developmental contexts. We found key differences between the two networks in both the genes employed, and in the regulation and function of common genes. These differences in the GRNs reveal why mutations in svb are unlikely to contribute to leg trichome evolution and how instead miR-92a represents the key evolutionary switch in this context. Our work shows that variability in GRNs across different developmental contexts, as well as whether a morphological feature is lost versus gained, influence the nodes at which a GRN evolves to cause morphological change. Therefore, our findings have important implications for understanding the pathways and predictability of evolution. © 2018 Kittelmann et al. http://creativecommons.org/licenses/by/4.0/ |
| title |
Gene regulatory network architecture in different developmental contexts influences the genetic basis of morphological evolution |
| title_short |
Gene regulatory network architecture in different developmental contexts influences the genetic basis of morphological evolution |
| title_full |
Gene regulatory network architecture in different developmental contexts influences the genetic basis of morphological evolution |
| title_fullStr |
Gene regulatory network architecture in different developmental contexts influences the genetic basis of morphological evolution |
| title_full_unstemmed |
Gene regulatory network architecture in different developmental contexts influences the genetic basis of morphological evolution |
| title_sort |
gene regulatory network architecture in different developmental contexts influences the genetic basis of morphological evolution |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15537390_v14_n5_p_Kittelmann http://hdl.handle.net/20.500.12110/paper_15537390_v14_n5_p_Kittelmann |
| _version_ |
1768542195663503360 |