Use of capillary blood to diagnose hereditary spherocytosis

We studied 31 children with hemolytic anemia, or with positive family history for hereditary spherocytosis (HS), to assess the reliability of capillary blood samples for the diagnosis. HS was diagnosed in 20 patients. Cryohemolysis (CH) was positive in 94% and eosin-5'-maleimide flow cytometry...

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Publicado:	2012
Materias:	Capillary blood Flow cytometry Hereditary spherocytosis Hypertonic cryohemolysis Neonatal anemia Osmotic fragility bilirubin article bilirubin blood level blood group ABO incompatibility blood sampling blood smear blood volume capillary blood child clinical article cryohemolysis early diagnosis erythrocyte disorder family history flow cytometry hemolysis hemolytic anemia hereditary spherocytosis human infant newborn hemolytic disease osmosis preschool child priority journal reticulocyte count school child Anemia, Hemolytic Blood Specimen Collection Capillaries Child Child, Preschool Composite Resins Eosine Yellowish-(YS) Freezing Hematologic Tests Hemolysis Humans Infant Infant, Newborn Osmotic Fragility Sensitivity and Specificity Spherocytosis, Hereditary
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15455009_v59_n7_p1299_Crisp http://hdl.handle.net/20.500.12110/paper_15455009_v59_n7_p1299_Crisp
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15455009_v59_n7_p1299_Crisp
record_format	dspace
spelling	paper:paper_15455009_v59_n7_p1299_Crisp2023-06-08T16:21:11Z Use of capillary blood to diagnose hereditary spherocytosis Capillary blood Flow cytometry Hereditary spherocytosis Hypertonic cryohemolysis Neonatal anemia Osmotic fragility bilirubin article bilirubin blood level blood group ABO incompatibility blood sampling blood smear blood volume capillary blood child clinical article cryohemolysis early diagnosis erythrocyte disorder family history flow cytometry hemolysis hemolytic anemia hereditary spherocytosis human infant newborn hemolytic disease osmosis preschool child priority journal reticulocyte count school child Anemia, Hemolytic Blood Specimen Collection Capillaries Child Child, Preschool Composite Resins Eosine Yellowish-(YS) Freezing Hematologic Tests Hemolysis Humans Infant Infant, Newborn Osmotic Fragility Sensitivity and Specificity Spherocytosis, Hereditary We studied 31 children with hemolytic anemia, or with positive family history for hereditary spherocytosis (HS), to assess the reliability of capillary blood samples for the diagnosis. HS was diagnosed in 20 patients. Cryohemolysis (CH) was positive in 94% and eosin-5'-maleimide flow cytometry in 90% of them, whereas flow cytometric osmotic fragility was positive in 94%. Capillary blood sampling showed to be useful for the diagnosis. Simultaneous use of these three tests allows confirming diagnosis in 100% of patients. The use of very small blood volumes (300μl) allows an earlier diagnosis in neonates and small infants. © 2012 Wiley Periodicals, Inc. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15455009_v59_n7_p1299_Crisp http://hdl.handle.net/20.500.12110/paper_15455009_v59_n7_p1299_Crisp
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Capillary blood Flow cytometry Hereditary spherocytosis Hypertonic cryohemolysis Neonatal anemia Osmotic fragility bilirubin article bilirubin blood level blood group ABO incompatibility blood sampling blood smear blood volume capillary blood child clinical article cryohemolysis early diagnosis erythrocyte disorder family history flow cytometry hemolysis hemolytic anemia hereditary spherocytosis human infant newborn hemolytic disease osmosis preschool child priority journal reticulocyte count school child Anemia, Hemolytic Blood Specimen Collection Capillaries Child Child, Preschool Composite Resins Eosine Yellowish-(YS) Freezing Hematologic Tests Hemolysis Humans Infant Infant, Newborn Osmotic Fragility Sensitivity and Specificity Spherocytosis, Hereditary
spellingShingle	Capillary blood Flow cytometry Hereditary spherocytosis Hypertonic cryohemolysis Neonatal anemia Osmotic fragility bilirubin article bilirubin blood level blood group ABO incompatibility blood sampling blood smear blood volume capillary blood child clinical article cryohemolysis early diagnosis erythrocyte disorder family history flow cytometry hemolysis hemolytic anemia hereditary spherocytosis human infant newborn hemolytic disease osmosis preschool child priority journal reticulocyte count school child Anemia, Hemolytic Blood Specimen Collection Capillaries Child Child, Preschool Composite Resins Eosine Yellowish-(YS) Freezing Hematologic Tests Hemolysis Humans Infant Infant, Newborn Osmotic Fragility Sensitivity and Specificity Spherocytosis, Hereditary Use of capillary blood to diagnose hereditary spherocytosis
topic_facet	Capillary blood Flow cytometry Hereditary spherocytosis Hypertonic cryohemolysis Neonatal anemia Osmotic fragility bilirubin article bilirubin blood level blood group ABO incompatibility blood sampling blood smear blood volume capillary blood child clinical article cryohemolysis early diagnosis erythrocyte disorder family history flow cytometry hemolysis hemolytic anemia hereditary spherocytosis human infant newborn hemolytic disease osmosis preschool child priority journal reticulocyte count school child Anemia, Hemolytic Blood Specimen Collection Capillaries Child Child, Preschool Composite Resins Eosine Yellowish-(YS) Freezing Hematologic Tests Hemolysis Humans Infant Infant, Newborn Osmotic Fragility Sensitivity and Specificity Spherocytosis, Hereditary
description	We studied 31 children with hemolytic anemia, or with positive family history for hereditary spherocytosis (HS), to assess the reliability of capillary blood samples for the diagnosis. HS was diagnosed in 20 patients. Cryohemolysis (CH) was positive in 94% and eosin-5'-maleimide flow cytometry in 90% of them, whereas flow cytometric osmotic fragility was positive in 94%. Capillary blood sampling showed to be useful for the diagnosis. Simultaneous use of these three tests allows confirming diagnosis in 100% of patients. The use of very small blood volumes (300μl) allows an earlier diagnosis in neonates and small infants. © 2012 Wiley Periodicals, Inc.
title	Use of capillary blood to diagnose hereditary spherocytosis
title_short	Use of capillary blood to diagnose hereditary spherocytosis
title_full	Use of capillary blood to diagnose hereditary spherocytosis
title_fullStr	Use of capillary blood to diagnose hereditary spherocytosis
title_full_unstemmed	Use of capillary blood to diagnose hereditary spherocytosis
title_sort	use of capillary blood to diagnose hereditary spherocytosis
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15455009_v59_n7_p1299_Crisp http://hdl.handle.net/20.500.12110/paper_15455009_v59_n7_p1299_Crisp
_version_	1768544290588327936

Use of capillary blood to diagnose hereditary spherocytosis

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